Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1644 and Psest_1645 are separated by 194 nucleotides Psest_1645 and Psest_1646 overlap by 23 nucleotides Psest_1646 and Psest_1647 are separated by 177 nucleotides
Psest_1644: Psest_1644 - Erythromycin esterase homolog, at 1,747,561 to 1,748,895
_1644
Psest_1645: Psest_1645 - hypothetical protein, at 1,749,090 to 1,749,323
_1645
Psest_1646: Psest_1646 - Predicted ICC-like phosphoesterases, at 1,749,301 to 1,749,990
_1646
Psest_1647: Psest_1647 - Lhr-like helicases, at 1,750,168 to 1,752,759
_1647
Position (kb)
1749
1750 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1748.325 kb on - strand, within Psest_1644 at 1748.363 kb on + strand, within Psest_1644 at 1748.420 kb on - strand, within Psest_1644 at 1748.715 kb on - strand, within Psest_1644 at 1748.715 kb on - strand, within Psest_1644 at 1748.895 kb on + strand at 1748.896 kb on - strand at 1748.896 kb on - strand at 1748.968 kb on + strand at 1748.968 kb on + strand at 1748.969 kb on - strand at 1748.969 kb on - strand at 1748.969 kb on - strand at 1748.969 kb on - strand at 1748.977 kb on + strand at 1748.977 kb on + strand at 1749.049 kb on + strand at 1749.050 kb on - strand at 1749.052 kb on + strand at 1749.053 kb on - strand at 1749.111 kb on + strand at 1749.111 kb on + strand at 1749.111 kb on + strand at 1749.111 kb on + strand at 1749.111 kb on + strand at 1749.112 kb on - strand at 1749.112 kb on - strand at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.372 kb on - strand, within Psest_1646 at 1749.372 kb on - strand, within Psest_1646 at 1749.372 kb on - strand, within Psest_1646 at 1749.372 kb on - strand, within Psest_1646 at 1749.372 kb on - strand, within Psest_1646 at 1749.621 kb on + strand, within Psest_1646 at 1749.622 kb on - strand, within Psest_1646 at 1749.853 kb on + strand, within Psest_1646 at 1749.853 kb on + strand, within Psest_1646 at 1749.970 kb on + strand at 1750.542 kb on - strand, within Psest_1647 at 1750.638 kb on + strand, within Psest_1647 at 1750.638 kb on + strand, within Psest_1647 at 1750.639 kb on - strand, within Psest_1647 at 1750.639 kb on - strand, within Psest_1647 at 1750.639 kb on - strand, within Psest_1647 at 1750.639 kb on - strand, within Psest_1647 at 1750.976 kb on - strand, within Psest_1647
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 1,748,325 - Psest_1644 0.57 +1.0 1,748,363 + Psest_1644 0.60 +0.8 1,748,420 - Psest_1644 0.64 -0.7 1,748,715 - Psest_1644 0.86 -1.2 1,748,715 - Psest_1644 0.86 -0.6 1,748,895 + +1.4 1,748,896 - +0.5 1,748,896 - -0.9 1,748,968 + +2.3 1,748,968 + -0.6 1,748,969 - +1.2 1,748,969 - -0.8 1,748,969 - +0.4 1,748,969 - -1.1 1,748,977 + +1.0 1,748,977 + +1.3 1,749,049 + +0.6 1,749,050 - -0.4 1,749,052 + -0.3 1,749,053 - -0.9 1,749,111 + +0.5 1,749,111 + -2.5 1,749,111 + +0.8 1,749,111 + -0.5 1,749,111 + +0.2 1,749,112 - +1.1 1,749,112 - -3.0 1,749,371 + Psest_1646 0.10 -1.7 1,749,371 + Psest_1646 0.10 -0.2 1,749,371 + Psest_1646 0.10 -1.7 1,749,371 + Psest_1646 0.10 -0.3 1,749,371 + Psest_1646 0.10 -1.6 1,749,371 + Psest_1646 0.10 +0.2 1,749,371 + Psest_1646 0.10 +0.8 1,749,371 + Psest_1646 0.10 -1.5 1,749,372 - Psest_1646 0.10 -0.6 1,749,372 - Psest_1646 0.10 -0.5 1,749,372 - Psest_1646 0.10 -0.3 1,749,372 - Psest_1646 0.10 +0.4 1,749,372 - Psest_1646 0.10 -0.7 1,749,621 + Psest_1646 0.46 +0.6 1,749,622 - Psest_1646 0.47 -2.0 1,749,853 + Psest_1646 0.80 -0.3 1,749,853 + Psest_1646 0.80 -1.0 1,749,970 + -0.0 1,750,542 - Psest_1647 0.14 -0.7 1,750,638 + Psest_1647 0.18 -0.8 1,750,638 + Psest_1647 0.18 +0.7 1,750,639 - Psest_1647 0.18 -0.3 1,750,639 - Psest_1647 0.18 +1.5 1,750,639 - Psest_1647 0.18 +0.4 1,750,639 - Psest_1647 0.18 -0.2 1,750,976 - Psest_1647 0.31 -0.5
Or see this region's nucleotide sequence