Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1434 and Psest_1435 are separated by 62 nucleotides Psest_1435 and Psest_1436 are separated by 3 nucleotides Psest_1436 and Psest_1437 are separated by 135 nucleotides
Psest_1434: Psest_1434 - NADPH:quinone reductase and related Zn-dependent oxidoreductases, at 1,545,278 to 1,546,288
_1434
Psest_1435: Psest_1435 - Predicted SAM-dependent methyltransferases, at 1,546,351 to 1,547,289
_1435
Psest_1436: Psest_1436 - Uncharacterized protein conserved in bacteria, at 1,547,293 to 1,547,745
_1436
Psest_1437: Psest_1437 - Uncharacterized protein conserved in archaea, at 1,547,881 to 1,548,399
_1437
Position (kb)
1546
1547
1548 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1545.404 kb on + strand, within Psest_1434 at 1545.404 kb on + strand, within Psest_1434 at 1545.404 kb on + strand, within Psest_1434 at 1545.405 kb on - strand, within Psest_1434 at 1545.674 kb on + strand, within Psest_1434 at 1545.909 kb on - strand, within Psest_1434 at 1546.039 kb on + strand, within Psest_1434 at 1546.039 kb on + strand, within Psest_1434 at 1546.039 kb on + strand, within Psest_1434 at 1546.039 kb on + strand, within Psest_1434 at 1546.040 kb on - strand, within Psest_1434 at 1546.040 kb on - strand, within Psest_1434 at 1546.316 kb on - strand at 1546.316 kb on - strand at 1546.628 kb on - strand, within Psest_1435 at 1546.628 kb on - strand, within Psest_1435 at 1546.705 kb on + strand, within Psest_1435 at 1546.705 kb on + strand, within Psest_1435 at 1546.705 kb on + strand, within Psest_1435 at 1546.705 kb on + strand, within Psest_1435 at 1546.706 kb on - strand, within Psest_1435 at 1546.706 kb on - strand, within Psest_1435 at 1546.706 kb on - strand, within Psest_1435 at 1546.706 kb on - strand, within Psest_1435 at 1546.819 kb on + strand, within Psest_1435 at 1546.819 kb on + strand, within Psest_1435 at 1546.820 kb on - strand, within Psest_1435 at 1546.820 kb on - strand, within Psest_1435 at 1547.008 kb on + strand, within Psest_1435 at 1547.009 kb on - strand, within Psest_1435 at 1547.581 kb on + strand, within Psest_1436 at 1547.581 kb on + strand, within Psest_1436 at 1547.582 kb on - strand, within Psest_1436 at 1547.627 kb on + strand, within Psest_1436 at 1547.627 kb on + strand, within Psest_1436 at 1547.628 kb on - strand, within Psest_1436 at 1547.628 kb on - strand, within Psest_1436 at 1547.628 kb on - strand, within Psest_1436 at 1547.678 kb on + strand, within Psest_1436 at 1547.745 kb on + strand at 1547.746 kb on - strand at 1547.746 kb on - strand at 1548.209 kb on + strand, within Psest_1437 at 1548.209 kb on + strand, within Psest_1437 at 1548.210 kb on - strand, within Psest_1437
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 1,545,404 + Psest_1434 0.12 -0.6 1,545,404 + Psest_1434 0.12 -1.8 1,545,404 + Psest_1434 0.12 -0.3 1,545,405 - Psest_1434 0.13 +0.5 1,545,674 + Psest_1434 0.39 -1.2 1,545,909 - Psest_1434 0.62 +0.2 1,546,039 + Psest_1434 0.75 +3.8 1,546,039 + Psest_1434 0.75 -0.9 1,546,039 + Psest_1434 0.75 -3.1 1,546,039 + Psest_1434 0.75 -0.4 1,546,040 - Psest_1434 0.75 +0.9 1,546,040 - Psest_1434 0.75 -1.0 1,546,316 - +0.3 1,546,316 - -0.8 1,546,628 - Psest_1435 0.29 +0.8 1,546,628 - Psest_1435 0.29 +1.3 1,546,705 + Psest_1435 0.38 +0.8 1,546,705 + Psest_1435 0.38 +0.2 1,546,705 + Psest_1435 0.38 +1.3 1,546,705 + Psest_1435 0.38 -0.8 1,546,706 - Psest_1435 0.38 +1.6 1,546,706 - Psest_1435 0.38 +0.7 1,546,706 - Psest_1435 0.38 +1.2 1,546,706 - Psest_1435 0.38 +0.2 1,546,819 + Psest_1435 0.50 +0.8 1,546,819 + Psest_1435 0.50 +1.2 1,546,820 - Psest_1435 0.50 +0.0 1,546,820 - Psest_1435 0.50 +1.2 1,547,008 + Psest_1435 0.70 +0.9 1,547,009 - Psest_1435 0.70 -0.8 1,547,581 + Psest_1436 0.64 -2.2 1,547,581 + Psest_1436 0.64 +1.2 1,547,582 - Psest_1436 0.64 +0.7 1,547,627 + Psest_1436 0.74 +0.7 1,547,627 + Psest_1436 0.74 -0.1 1,547,628 - Psest_1436 0.74 -0.5 1,547,628 - Psest_1436 0.74 -1.4 1,547,628 - Psest_1436 0.74 +0.5 1,547,678 + Psest_1436 0.85 +0.4 1,547,745 + +0.0 1,547,746 - +1.9 1,547,746 - +0.1 1,548,209 + Psest_1437 0.63 +0.2 1,548,209 + Psest_1437 0.63 +2.0 1,548,210 - Psest_1437 0.63 +1.0
Or see this region's nucleotide sequence