Experiment: Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1160 and Psest_1161 are separated by 1 nucleotides Psest_1161 and Psest_1162 are separated by 14 nucleotides Psest_1162 and Psest_1163 are separated by 125 nucleotides
Psest_1160: Psest_1160 - Uncharacterized conserved protein, at 1,261,184 to 1,261,756
_1160
Psest_1161: Psest_1161 - E3 Ubiquitin ligase., at 1,261,758 to 1,262,651
_1161
Psest_1162: Psest_1162 - Spermidine synthase, at 1,262,666 to 1,263,349
_1162
Psest_1163: Psest_1163 - diguanylate cyclase (GGDEF) domain, at 1,263,475 to 1,265,535
_1163
Position (kb)
1262
1263
1264 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1261.899 kb on + strand, within Psest_1161 at 1261.900 kb on - strand, within Psest_1161 at 1261.900 kb on - strand, within Psest_1161 at 1262.260 kb on - strand, within Psest_1161 at 1262.636 kb on + strand at 1262.636 kb on + strand at 1263.078 kb on - strand, within Psest_1162 at 1263.078 kb on - strand, within Psest_1162 at 1263.156 kb on - strand, within Psest_1162 at 1263.336 kb on - strand at 1263.336 kb on - strand at 1263.336 kb on - strand at 1263.448 kb on - strand at 1264.212 kb on - strand, within Psest_1163
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.004 mg/ml remove 1,261,899 + Psest_1161 0.16 -0.7 1,261,900 - Psest_1161 0.16 -2.2 1,261,900 - Psest_1161 0.16 -0.6 1,262,260 - Psest_1161 0.56 -1.5 1,262,636 + -0.0 1,262,636 + +0.2 1,263,078 - Psest_1162 0.60 -1.4 1,263,078 - Psest_1162 0.60 -1.8 1,263,156 - Psest_1162 0.72 -0.1 1,263,336 - +2.6 1,263,336 - +1.4 1,263,336 - -0.5 1,263,448 - -2.0 1,264,212 - Psest_1163 0.36 -1.8
Or see this region's nucleotide sequence