Experiment: D-Cycloserine 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0800 and Psest_0801 are separated by 336 nucleotides Psest_0801 and Psest_0802 are separated by 10 nucleotides Psest_0802 and Psest_0803 are separated by 70 nucleotides
Psest_0800: Psest_0800 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 874,734 to 875,477
_0800
Psest_0801: Psest_0801 - CBS-domain-containing membrane protein, at 875,814 to 876,950
_0801
Psest_0802: Psest_0802 - Transcriptional regulator, at 876,961 to 877,821
_0802
Psest_0803: Psest_0803 - prepilin-type N-terminal cleavage/methylation domain, at 877,892 to 878,419
_0803
Position (kb)
875
876
877 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 875.078 kb on + strand, within Psest_0800 at 875.078 kb on + strand, within Psest_0800 at 875.079 kb on - strand, within Psest_0800 at 875.175 kb on + strand, within Psest_0800 at 875.175 kb on + strand, within Psest_0800 at 875.175 kb on + strand, within Psest_0800 at 875.175 kb on + strand, within Psest_0800 at 875.175 kb on + strand, within Psest_0800 at 875.175 kb on + strand, within Psest_0800 at 875.175 kb on + strand, within Psest_0800 at 875.175 kb on + strand, within Psest_0800 at 875.176 kb on - strand, within Psest_0800 at 875.176 kb on - strand, within Psest_0800 at 875.176 kb on - strand, within Psest_0800 at 875.176 kb on - strand, within Psest_0800 at 875.176 kb on - strand, within Psest_0800 at 875.176 kb on - strand, within Psest_0800 at 875.178 kb on - strand, within Psest_0800 at 875.249 kb on + strand, within Psest_0800 at 875.465 kb on + strand at 875.465 kb on + strand at 875.465 kb on + strand at 875.465 kb on + strand at 875.465 kb on + strand at 875.465 kb on + strand at 875.466 kb on - strand at 875.466 kb on - strand at 875.466 kb on - strand at 875.466 kb on - strand at 875.466 kb on - strand at 875.466 kb on - strand at 875.466 kb on - strand at 875.598 kb on + strand at 875.599 kb on - strand at 876.303 kb on + strand, within Psest_0801 at 876.303 kb on + strand, within Psest_0801 at 876.303 kb on + strand, within Psest_0801 at 876.304 kb on - strand, within Psest_0801 at 876.304 kb on - strand, within Psest_0801 at 876.304 kb on - strand, within Psest_0801 at 876.304 kb on - strand, within Psest_0801 at 876.304 kb on - strand, within Psest_0801 at 876.304 kb on - strand, within Psest_0801 at 876.444 kb on + strand, within Psest_0801 at 876.445 kb on - strand, within Psest_0801 at 876.445 kb on - strand, within Psest_0801 at 876.445 kb on - strand, within Psest_0801 at 876.558 kb on + strand, within Psest_0801 at 876.558 kb on + strand, within Psest_0801 at 876.559 kb on - strand, within Psest_0801 at 876.819 kb on + strand, within Psest_0801 at 876.819 kb on + strand, within Psest_0801 at 876.819 kb on + strand, within Psest_0801 at 876.820 kb on - strand, within Psest_0801 at 876.820 kb on - strand, within Psest_0801 at 876.972 kb on - strand at 877.073 kb on + strand, within Psest_0802 at 877.074 kb on - strand, within Psest_0802 at 877.074 kb on - strand, within Psest_0802 at 877.082 kb on + strand, within Psest_0802 at 877.160 kb on + strand, within Psest_0802 at 877.218 kb on + strand, within Psest_0802 at 877.218 kb on + strand, within Psest_0802 at 877.218 kb on + strand, within Psest_0802 at 877.218 kb on + strand, within Psest_0802 at 877.289 kb on + strand, within Psest_0802 at 877.289 kb on + strand, within Psest_0802 at 877.289 kb on + strand, within Psest_0802 at 877.289 kb on + strand, within Psest_0802 at 877.289 kb on + strand, within Psest_0802 at 877.289 kb on + strand, within Psest_0802 at 877.290 kb on - strand, within Psest_0802 at 877.290 kb on - strand, within Psest_0802 at 877.290 kb on - strand, within Psest_0802 at 877.290 kb on - strand, within Psest_0802 at 877.290 kb on - strand, within Psest_0802 at 877.290 kb on - strand, within Psest_0802 at 877.290 kb on - strand, within Psest_0802 at 877.290 kb on - strand, within Psest_0802 at 877.290 kb on - strand, within Psest_0802 at 877.290 kb on - strand, within Psest_0802 at 877.295 kb on + strand, within Psest_0802 at 877.295 kb on + strand, within Psest_0802 at 877.295 kb on + strand, within Psest_0802 at 877.295 kb on + strand, within Psest_0802 at 877.295 kb on + strand, within Psest_0802 at 877.295 kb on + strand, within Psest_0802 at 877.296 kb on - strand, within Psest_0802 at 877.296 kb on - strand, within Psest_0802 at 877.296 kb on - strand, within Psest_0802 at 877.374 kb on - strand, within Psest_0802 at 877.495 kb on + strand, within Psest_0802 at 877.622 kb on + strand, within Psest_0802 at 877.622 kb on + strand, within Psest_0802 at 877.623 kb on - strand, within Psest_0802 at 877.743 kb on + strand at 877.743 kb on + strand at 877.855 kb on + strand at 877.855 kb on + strand at 877.855 kb on + strand at 877.855 kb on + strand at 877.856 kb on - strand at 877.856 kb on - strand at 877.856 kb on - strand at 877.856 kb on - strand at 877.883 kb on + strand at 877.884 kb on - strand at 877.887 kb on + strand at 877.887 kb on + strand at 877.887 kb on + strand at 877.887 kb on + strand at 877.887 kb on + strand at 877.888 kb on - strand at 877.888 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.032 mg/ml remove 875,078 + Psest_0800 0.46 +0.1 875,078 + Psest_0800 0.46 +0.2 875,079 - Psest_0800 0.46 -1.1 875,175 + Psest_0800 0.59 +1.7 875,175 + Psest_0800 0.59 +2.0 875,175 + Psest_0800 0.59 -0.7 875,175 + Psest_0800 0.59 +0.7 875,175 + Psest_0800 0.59 -1.7 875,175 + Psest_0800 0.59 -1.5 875,175 + Psest_0800 0.59 +2.7 875,175 + Psest_0800 0.59 -2.9 875,176 - Psest_0800 0.59 -1.3 875,176 - Psest_0800 0.59 -2.0 875,176 - Psest_0800 0.59 -0.4 875,176 - Psest_0800 0.59 -0.2 875,176 - Psest_0800 0.59 -0.2 875,176 - Psest_0800 0.59 -0.3 875,178 - Psest_0800 0.60 +2.0 875,249 + Psest_0800 0.69 -1.0 875,465 + -2.3 875,465 + +1.1 875,465 + -0.5 875,465 + +1.2 875,465 + -1.1 875,465 + +2.2 875,466 - +0.4 875,466 - +0.0 875,466 - -1.0 875,466 - -0.4 875,466 - -2.6 875,466 - -2.3 875,466 - -2.1 875,598 + -1.8 875,599 - +2.3 876,303 + Psest_0801 0.43 -3.0 876,303 + Psest_0801 0.43 +1.0 876,303 + Psest_0801 0.43 +1.7 876,304 - Psest_0801 0.43 -0.3 876,304 - Psest_0801 0.43 -1.9 876,304 - Psest_0801 0.43 -1.5 876,304 - Psest_0801 0.43 -2.9 876,304 - Psest_0801 0.43 +1.0 876,304 - Psest_0801 0.43 -0.6 876,444 + Psest_0801 0.55 -0.8 876,445 - Psest_0801 0.55 +0.3 876,445 - Psest_0801 0.55 -2.6 876,445 - Psest_0801 0.55 +0.4 876,558 + Psest_0801 0.65 -2.7 876,558 + Psest_0801 0.65 -0.1 876,559 - Psest_0801 0.66 +0.0 876,819 + Psest_0801 0.88 -1.1 876,819 + Psest_0801 0.88 -1.4 876,819 + Psest_0801 0.88 -1.6 876,820 - Psest_0801 0.88 -0.3 876,820 - Psest_0801 0.88 -1.7 876,972 - -1.9 877,073 + Psest_0802 0.13 -1.8 877,074 - Psest_0802 0.13 -1.5 877,074 - Psest_0802 0.13 -0.5 877,082 + Psest_0802 0.14 -0.4 877,160 + Psest_0802 0.23 -0.9 877,218 + Psest_0802 0.30 -0.7 877,218 + Psest_0802 0.30 -1.2 877,218 + Psest_0802 0.30 -0.5 877,218 + Psest_0802 0.30 +1.2 877,289 + Psest_0802 0.38 -0.1 877,289 + Psest_0802 0.38 -0.9 877,289 + Psest_0802 0.38 -1.0 877,289 + Psest_0802 0.38 -1.6 877,289 + Psest_0802 0.38 +0.7 877,289 + Psest_0802 0.38 +1.9 877,290 - Psest_0802 0.38 -0.5 877,290 - Psest_0802 0.38 +0.2 877,290 - Psest_0802 0.38 -2.2 877,290 - Psest_0802 0.38 +0.5 877,290 - Psest_0802 0.38 -2.2 877,290 - Psest_0802 0.38 -0.1 877,290 - Psest_0802 0.38 -0.5 877,290 - Psest_0802 0.38 -1.7 877,290 - Psest_0802 0.38 -1.4 877,290 - Psest_0802 0.38 -0.1 877,295 + Psest_0802 0.39 +0.3 877,295 + Psest_0802 0.39 +0.6 877,295 + Psest_0802 0.39 -0.9 877,295 + Psest_0802 0.39 +4.0 877,295 + Psest_0802 0.39 +0.8 877,295 + Psest_0802 0.39 -1.6 877,296 - Psest_0802 0.39 -0.3 877,296 - Psest_0802 0.39 -0.2 877,296 - Psest_0802 0.39 -0.8 877,374 - Psest_0802 0.48 +1.9 877,495 + Psest_0802 0.62 -0.5 877,622 + Psest_0802 0.77 -0.5 877,622 + Psest_0802 0.77 -1.0 877,623 - Psest_0802 0.77 +0.1 877,743 + -1.8 877,743 + +0.3 877,855 + +0.7 877,855 + -0.1 877,855 + +0.4 877,855 + +1.1 877,856 - -1.3 877,856 - -2.0 877,856 - +0.1 877,856 - +0.6 877,883 + -0.8 877,884 - +2.6 877,887 + +1.5 877,887 + +1.0 877,887 + -0.7 877,887 + +0.7 877,887 + -0.3 877,888 - +1.1 877,888 - +0.9
Or see this region's nucleotide sequence