Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0666

Experiment: D-Cycloserine 0.032 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0664 and Psest_0665 are separated by 84 nucleotidesPsest_0665 and Psest_0666 are separated by 105 nucleotidesPsest_0666 and Psest_0667 are separated by 147 nucleotides Psest_0664: Psest_0664 - diguanylate cyclase (GGDEF) domain, at 723,162 to 724,652 _0664 Psest_0665: Psest_0665 - Inorganic pyrophosphatase, at 724,737 to 725,264 _0665 Psest_0666: Psest_0666 - Uncharacterized protein conserved in bacteria, at 725,370 to 726,182 _0666 Psest_0667: Psest_0667 - Phosphodiesterase/alkaline phosphatase D, at 726,330 to 727,883 _0667 Position (kb) 725 726 727Strain fitness (log2 ratio) -2 -1 0 1 2 3at 724.490 kb on - strand, within Psest_0664at 724.548 kb on + strandat 724.548 kb on + strandat 724.549 kb on - strandat 724.576 kb on + strandat 724.578 kb on + strandat 724.578 kb on + strandat 724.578 kb on + strandat 724.578 kb on + strandat 724.578 kb on + strandat 724.579 kb on - strandat 724.579 kb on - strandat 724.579 kb on - strandat 724.579 kb on - strandat 724.579 kb on - strandat 724.611 kb on + strandat 724.611 kb on + strandat 724.738 kb on + strandat 724.738 kb on + strandat 724.738 kb on + strandat 724.739 kb on - strandat 724.739 kb on - strandat 725.326 kb on - strandat 725.371 kb on + strandat 725.372 kb on - strandat 725.461 kb on + strand, within Psest_0666at 725.462 kb on - strand, within Psest_0666at 725.475 kb on + strand, within Psest_0666at 725.475 kb on + strand, within Psest_0666at 725.476 kb on - strand, within Psest_0666at 725.476 kb on - strand, within Psest_0666at 725.509 kb on + strand, within Psest_0666at 725.509 kb on + strand, within Psest_0666at 725.510 kb on - strand, within Psest_0666at 725.614 kb on + strand, within Psest_0666at 725.737 kb on + strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 726.033 kb on + strand, within Psest_0666at 726.033 kb on + strand, within Psest_0666at 726.033 kb on + strand, within Psest_0666at 726.033 kb on + strand, within Psest_0666at 726.033 kb on + strand, within Psest_0666at 726.034 kb on - strand, within Psest_0666at 726.034 kb on - strand, within Psest_0666at 726.216 kb on + strandat 726.216 kb on + strandat 726.217 kb on - strandat 726.217 kb on - strandat 726.217 kb on - strandat 726.217 kb on - strandat 726.217 kb on - strandat 726.250 kb on + strandat 726.251 kb on - strandat 726.293 kb on - strandat 726.822 kb on + strand, within Psest_0667at 726.822 kb on + strand, within Psest_0667at 726.822 kb on + strand, within Psest_0667at 726.823 kb on - strand, within Psest_0667at 726.823 kb on - strand, within Psest_0667at 726.823 kb on - strand, within Psest_0667at 726.823 kb on - strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.880 kb on - strand, within Psest_0667at 726.880 kb on - strand, within Psest_0667at 726.880 kb on - strand, within Psest_0667at 726.996 kb on + strand, within Psest_0667at 726.996 kb on + strand, within Psest_0667at 726.997 kb on - strand, within Psest_0667at 727.002 kb on + strand, within Psest_0667

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.032 mg/ml
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724,490 - Psest_0664 0.89 -2.7
724,548 + -2.1
724,548 + -0.2
724,549 - -0.0
724,576 + +0.6
724,578 + +1.5
724,578 + -0.3
724,578 + +1.3
724,578 + +0.9
724,578 + -0.8
724,579 - -0.3
724,579 - +1.2
724,579 - +0.1
724,579 - -0.6
724,579 - +1.4
724,611 + +0.2
724,611 + -1.2
724,738 + -2.5
724,738 + -1.5
724,738 + -2.2
724,739 - -0.2
724,739 - -1.1
725,326 - +1.5
725,371 + -0.7
725,372 - +0.3
725,461 + Psest_0666 0.11 -1.1
725,462 - Psest_0666 0.11 -1.7
725,475 + Psest_0666 0.13 +1.5
725,475 + Psest_0666 0.13 -2.0
725,476 - Psest_0666 0.13 -1.3
725,476 - Psest_0666 0.13 +0.9
725,509 + Psest_0666 0.17 -2.5
725,509 + Psest_0666 0.17 -0.2
725,510 - Psest_0666 0.17 +0.4
725,614 + Psest_0666 0.30 -1.3
725,737 + Psest_0666 0.45 -2.1
725,738 - Psest_0666 0.45 -2.0
725,738 - Psest_0666 0.45 -0.2
725,738 - Psest_0666 0.45 -1.5
725,738 - Psest_0666 0.45 -0.0
725,738 - Psest_0666 0.45 +0.7
725,738 - Psest_0666 0.45 -1.9
725,738 - Psest_0666 0.45 +1.4
726,033 + Psest_0666 0.82 -0.8
726,033 + Psest_0666 0.82 -0.9
726,033 + Psest_0666 0.82 +0.4
726,033 + Psest_0666 0.82 +0.7
726,033 + Psest_0666 0.82 +1.1
726,034 - Psest_0666 0.82 -0.6
726,034 - Psest_0666 0.82 -0.1
726,216 + -1.1
726,216 + +1.5
726,217 - -1.4
726,217 - -2.2
726,217 - -0.3
726,217 - +1.7
726,217 - +0.0
726,250 + +1.6
726,251 - -0.1
726,293 - +0.1
726,822 + Psest_0667 0.32 +2.0
726,822 + Psest_0667 0.32 +0.1
726,822 + Psest_0667 0.32 -0.0
726,823 - Psest_0667 0.32 +0.3
726,823 - Psest_0667 0.32 -0.0
726,823 - Psest_0667 0.32 +0.4
726,823 - Psest_0667 0.32 +0.6
726,879 + Psest_0667 0.35 -1.4
726,879 + Psest_0667 0.35 +0.9
726,879 + Psest_0667 0.35 +0.3
726,879 + Psest_0667 0.35 -1.0
726,879 + Psest_0667 0.35 +0.5
726,879 + Psest_0667 0.35 +1.0
726,879 + Psest_0667 0.35 -0.4
726,879 + Psest_0667 0.35 +3.0
726,880 - Psest_0667 0.35 -1.9
726,880 - Psest_0667 0.35 -1.5
726,880 - Psest_0667 0.35 +1.0
726,996 + Psest_0667 0.43 -2.4
726,996 + Psest_0667 0.43 +2.4
726,997 - Psest_0667 0.43 +0.3
727,002 + Psest_0667 0.43 +0.6

Or see this region's nucleotide sequence