Experiment: D-Cycloserine 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3121 and Psest_3122 are separated by 59 nucleotides Psest_3122 and Psest_3123 are separated by 162 nucleotides
Psest_3121: Psest_3121 - argininosuccinate synthase, at 3,297,276 to 3,298,493
_3121
Psest_3122: Psest_3122 - Outer membrane protein and related peptidoglycan-associated (lipo)proteins, at 3,298,553 to 3,299,470
_3122
Psest_3123: Psest_3123 - dihydroorotase, homodimeric type, at 3,299,633 to 3,300,676
_3123
Position (kb)
3298
3299
3300 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3298.515 kb on - strand at 3298.555 kb on - strand at 3298.555 kb on - strand at 3298.621 kb on + strand at 3298.621 kb on + strand at 3298.758 kb on + strand, within Psest_3122 at 3298.759 kb on - strand, within Psest_3122 at 3298.844 kb on + strand, within Psest_3122 at 3298.845 kb on - strand, within Psest_3122 at 3298.845 kb on - strand, within Psest_3122 at 3298.845 kb on - strand, within Psest_3122 at 3298.889 kb on - strand, within Psest_3122 at 3298.993 kb on + strand, within Psest_3122 at 3299.056 kb on + strand, within Psest_3122 at 3299.056 kb on + strand, within Psest_3122 at 3299.057 kb on - strand, within Psest_3122 at 3299.492 kb on + strand at 3299.492 kb on + strand at 3299.492 kb on + strand at 3299.493 kb on - strand at 3299.501 kb on + strand at 3299.501 kb on + strand at 3299.502 kb on - strand at 3299.502 kb on - strand at 3299.502 kb on - strand at 3299.502 kb on - strand at 3299.502 kb on - strand at 3299.502 kb on - strand at 3299.861 kb on + strand, within Psest_3123 at 3299.861 kb on + strand, within Psest_3123 at 3300.108 kb on + strand, within Psest_3123 at 3300.108 kb on + strand, within Psest_3123 at 3300.431 kb on + strand, within Psest_3123 at 3300.433 kb on + strand, within Psest_3123 at 3300.434 kb on - strand, within Psest_3123 at 3300.458 kb on + strand, within Psest_3123 at 3300.458 kb on + strand, within Psest_3123 at 3300.458 kb on + strand, within Psest_3123 at 3300.459 kb on - strand, within Psest_3123
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.032 mg/ml remove 3,298,515 - -0.1 3,298,555 - -2.0 3,298,555 - +0.6 3,298,621 + -0.8 3,298,621 + -0.3 3,298,758 + Psest_3122 0.22 -0.9 3,298,759 - Psest_3122 0.22 +0.2 3,298,844 + Psest_3122 0.32 -1.5 3,298,845 - Psest_3122 0.32 -0.3 3,298,845 - Psest_3122 0.32 +0.4 3,298,845 - Psest_3122 0.32 -2.3 3,298,889 - Psest_3122 0.37 -3.2 3,298,993 + Psest_3122 0.48 -2.6 3,299,056 + Psest_3122 0.55 +1.5 3,299,056 + Psest_3122 0.55 -0.7 3,299,057 - Psest_3122 0.55 -1.6 3,299,492 + -1.1 3,299,492 + -0.3 3,299,492 + -0.9 3,299,493 - +1.0 3,299,501 + +2.5 3,299,501 + +1.4 3,299,502 - +0.8 3,299,502 - +1.2 3,299,502 - -0.7 3,299,502 - -2.0 3,299,502 - +0.7 3,299,502 - +0.9 3,299,861 + Psest_3123 0.22 +1.7 3,299,861 + Psest_3123 0.22 +0.6 3,300,108 + Psest_3123 0.45 +1.7 3,300,108 + Psest_3123 0.45 +1.7 3,300,431 + Psest_3123 0.76 +1.7 3,300,433 + Psest_3123 0.77 +3.0 3,300,434 - Psest_3123 0.77 +1.7 3,300,458 + Psest_3123 0.79 +1.7 3,300,458 + Psest_3123 0.79 +1.7 3,300,458 + Psest_3123 0.79 +1.7 3,300,459 - Psest_3123 0.79 +1.7
Or see this region's nucleotide sequence