Experiment: D-Cycloserine 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0065 and Psest_0066 are separated by 276 nucleotides Psest_0066 and Psest_0067 are separated by 8 nucleotides Psest_0067 and Psest_0068 are separated by 101 nucleotides Psest_0068 and Psest_0069 are separated by 79 nucleotides
Psest_0065: Psest_0065 - Subtilisin-like serine proteases, at 65,325 to 66,716
_0065
Psest_0066: Psest_0066 - Protein of unknown function (DUF3060)., at 66,993 to 67,493
_0066
Psest_0067: Psest_0067 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 67,502 to 68,500
_0067
Psest_0068: Psest_0068 - Amidases related to nicotinamidase, at 68,602 to 69,156
_0068
Psest_0069: Psest_0069 - UDP-glucose-4-epimerase, at 69,236 to 70,300
_0069
Position (kb) 67
68
69 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 66.507 kb on + strand, within Psest_0065 at 66.507 kb on + strand, within Psest_0065 at 66.507 kb on + strand, within Psest_0065 at 66.507 kb on + strand, within Psest_0065 at 66.508 kb on - strand, within Psest_0065 at 66.508 kb on - strand, within Psest_0065 at 66.508 kb on - strand, within Psest_0065 at 66.561 kb on + strand, within Psest_0065 at 66.562 kb on - strand, within Psest_0065 at 66.572 kb on - strand, within Psest_0065 at 66.652 kb on + strand at 66.719 kb on + strand at 66.719 kb on + strand at 66.719 kb on + strand at 66.719 kb on + strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.723 kb on + strand at 66.724 kb on - strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.857 kb on - strand at 66.857 kb on - strand at 67.421 kb on + strand, within Psest_0066 at 67.421 kb on + strand, within Psest_0066 at 67.421 kb on + strand at 67.422 kb on - strand, within Psest_0066 at 67.422 kb on - strand, within Psest_0066 at 67.699 kb on - strand, within Psest_0067 at 67.835 kb on + strand, within Psest_0067 at 67.917 kb on + strand, within Psest_0067 at 68.519 kb on - strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.559 kb on + strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.560 kb on - strand at 68.645 kb on - strand at 68.645 kb on - strand at 69.136 kb on + strand at 69.136 kb on + strand at 69.210 kb on + strand at 69.210 kb on + strand at 69.210 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.032 mg/ml remove 66,507 + Psest_0065 0.85 +0.3 66,507 + Psest_0065 0.85 +0.1 66,507 + Psest_0065 0.85 +0.4 66,507 + Psest_0065 0.85 +1.7 66,508 - Psest_0065 0.85 +0.3 66,508 - Psest_0065 0.85 +0.4 66,508 - Psest_0065 0.85 -0.5 66,561 + Psest_0065 0.89 +0.6 66,562 - Psest_0065 0.89 +1.4 66,572 - Psest_0065 0.90 +0.8 66,652 + +1.9 66,719 + -1.5 66,719 + +2.5 66,719 + +0.1 66,719 + +0.7 66,720 - +0.0 66,720 - +0.5 66,723 + -4.1 66,724 - -1.1 66,856 + +0.7 66,856 + -0.6 66,856 + -0.2 66,856 + -2.7 66,856 + -2.5 66,856 + -0.4 66,856 + -2.0 66,857 - +1.0 66,857 - +0.7 67,421 + Psest_0066 0.85 +1.0 67,421 + Psest_0066 0.85 +0.0 67,421 + +2.7 67,422 - Psest_0066 0.86 +0.2 67,422 - Psest_0066 0.86 +0.5 67,699 - Psest_0067 0.20 +0.1 67,835 + Psest_0067 0.33 +0.2 67,917 + Psest_0067 0.42 +2.2 68,519 - +1.7 68,559 + -2.8 68,559 + -0.1 68,559 + +1.7 68,559 + -1.1 68,559 + +2.0 68,559 + -3.0 68,559 + +0.3 68,559 + -0.9 68,559 + -2.8 68,559 + +0.4 68,559 + +1.2 68,559 + -1.7 68,559 + +0.7 68,559 + -2.7 68,559 + -1.5 68,560 - +0.4 68,560 - -0.3 68,560 - +1.7 68,560 - +0.1 68,560 - +0.2 68,560 - -0.4 68,560 - -0.8 68,560 - +1.7 68,560 - +0.4 68,560 - -1.0 68,560 - +1.2 68,560 - -0.1 68,560 - -0.6 68,560 - -1.4 68,560 - -1.7 68,560 - -2.3 68,645 - +1.3 68,645 - -1.6 69,136 + -2.1 69,136 + +1.5 69,210 + -0.3 69,210 + +1.2 69,210 + -1.3
Or see this region's nucleotide sequence