Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0065

Experiment: D-Cycloserine 0.032 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0064 and Psest_0065 are separated by 271 nucleotidesPsest_0065 and Psest_0066 are separated by 276 nucleotidesPsest_0066 and Psest_0067 are separated by 8 nucleotides Psest_0064: Psest_0064 - Transcriptional regulator, at 64,151 to 65,053 _0064 Psest_0065: Psest_0065 - Subtilisin-like serine proteases, at 65,325 to 66,716 _0065 Psest_0066: Psest_0066 - Protein of unknown function (DUF3060)., at 66,993 to 67,493 _0066 Psest_0067: Psest_0067 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 67,502 to 68,500 _0067 Position (kb) 65 66 67Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5at 64.829 kb on + strand, within Psest_0064at 64.829 kb on + strand, within Psest_0064at 64.829 kb on + strand, within Psest_0064at 64.829 kb on + strand, within Psest_0064at 64.830 kb on - strand, within Psest_0064at 64.830 kb on - strand, within Psest_0064at 64.830 kb on - strand, within Psest_0064at 64.830 kb on - strand, within Psest_0064at 64.830 kb on - strand, within Psest_0064at 65.099 kb on + strandat 65.099 kb on + strandat 65.179 kb on + strandat 65.246 kb on + strandat 65.246 kb on + strandat 65.247 kb on - strandat 65.247 kb on - strandat 65.247 kb on - strandat 65.269 kb on + strandat 65.269 kb on + strandat 65.269 kb on + strandat 65.270 kb on - strandat 65.270 kb on - strandat 65.270 kb on - strandat 65.270 kb on - strandat 65.683 kb on - strand, within Psest_0065at 65.724 kb on - strand, within Psest_0065at 65.769 kb on + strand, within Psest_0065at 65.994 kb on + strand, within Psest_0065at 66.162 kb on + strand, within Psest_0065at 66.162 kb on + strand, within Psest_0065at 66.162 kb on + strand, within Psest_0065at 66.162 kb on + strand, within Psest_0065at 66.162 kb on + strand, within Psest_0065at 66.163 kb on - strand, within Psest_0065at 66.163 kb on - strand, within Psest_0065at 66.250 kb on - strand, within Psest_0065at 66.250 kb on - strand, within Psest_0065at 66.351 kb on + strand, within Psest_0065at 66.351 kb on + strand, within Psest_0065at 66.351 kb on + strand, within Psest_0065at 66.352 kb on - strand, within Psest_0065at 66.352 kb on - strand, within Psest_0065at 66.352 kb on - strand, within Psest_0065at 66.420 kb on + strand, within Psest_0065at 66.421 kb on - strand, within Psest_0065at 66.421 kb on - strand, within Psest_0065at 66.507 kb on + strand, within Psest_0065at 66.507 kb on + strand, within Psest_0065at 66.507 kb on + strand, within Psest_0065at 66.507 kb on + strand, within Psest_0065at 66.508 kb on - strand, within Psest_0065at 66.508 kb on - strand, within Psest_0065at 66.508 kb on - strand, within Psest_0065at 66.561 kb on + strand, within Psest_0065at 66.562 kb on - strand, within Psest_0065at 66.572 kb on - strand, within Psest_0065at 66.652 kb on + strandat 66.719 kb on + strandat 66.719 kb on + strandat 66.719 kb on + strandat 66.719 kb on + strandat 66.720 kb on - strandat 66.720 kb on - strandat 66.723 kb on + strandat 66.724 kb on - strandat 66.856 kb on + strandat 66.856 kb on + strandat 66.856 kb on + strandat 66.856 kb on + strandat 66.856 kb on + strandat 66.856 kb on + strandat 66.856 kb on + strandat 66.857 kb on - strandat 66.857 kb on - strandat 67.421 kb on + strand, within Psest_0066at 67.421 kb on + strand, within Psest_0066at 67.421 kb on + strandat 67.422 kb on - strand, within Psest_0066at 67.422 kb on - strand, within Psest_0066at 67.699 kb on - strand, within Psest_0067

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.032 mg/ml
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64,829 + Psest_0064 0.75 +0.6
64,829 + Psest_0064 0.75 +1.9
64,829 + Psest_0064 0.75 +1.2
64,829 + Psest_0064 0.75 +2.0
64,830 - Psest_0064 0.75 +2.7
64,830 - Psest_0064 0.75 +1.7
64,830 - Psest_0064 0.75 +0.6
64,830 - Psest_0064 0.75 +2.6
64,830 - Psest_0064 0.75 +0.6
65,099 + -1.1
65,099 + -2.1
65,179 + -1.9
65,246 + -0.6
65,246 + +0.5
65,247 - +2.0
65,247 - -0.9
65,247 - +1.3
65,269 + -1.4
65,269 + -0.2
65,269 + +1.0
65,270 - +1.6
65,270 - +1.1
65,270 - +5.0
65,270 - +2.6
65,683 - Psest_0065 0.26 -1.6
65,724 - Psest_0065 0.29 +0.8
65,769 + Psest_0065 0.32 +1.4
65,994 + Psest_0065 0.48 +0.9
66,162 + Psest_0065 0.60 +0.8
66,162 + Psest_0065 0.60 -0.5
66,162 + Psest_0065 0.60 +0.5
66,162 + Psest_0065 0.60 +3.3
66,162 + Psest_0065 0.60 +2.7
66,163 - Psest_0065 0.60 +2.4
66,163 - Psest_0065 0.60 -1.2
66,250 - Psest_0065 0.66 -1.6
66,250 - Psest_0065 0.66 +1.2
66,351 + Psest_0065 0.74 +0.0
66,351 + Psest_0065 0.74 +2.2
66,351 + Psest_0065 0.74 +1.2
66,352 - Psest_0065 0.74 -0.1
66,352 - Psest_0065 0.74 +1.6
66,352 - Psest_0065 0.74 +2.5
66,420 + Psest_0065 0.79 +0.2
66,421 - Psest_0065 0.79 +0.2
66,421 - Psest_0065 0.79 +0.8
66,507 + Psest_0065 0.85 +0.3
66,507 + Psest_0065 0.85 +0.1
66,507 + Psest_0065 0.85 +0.4
66,507 + Psest_0065 0.85 +1.7
66,508 - Psest_0065 0.85 +0.3
66,508 - Psest_0065 0.85 +0.4
66,508 - Psest_0065 0.85 -0.5
66,561 + Psest_0065 0.89 +0.6
66,562 - Psest_0065 0.89 +1.4
66,572 - Psest_0065 0.90 +0.8
66,652 + +1.9
66,719 + -1.5
66,719 + +2.5
66,719 + +0.1
66,719 + +0.7
66,720 - +0.0
66,720 - +0.5
66,723 + -4.1
66,724 - -1.1
66,856 + +0.7
66,856 + -0.6
66,856 + -0.2
66,856 + -2.7
66,856 + -2.5
66,856 + -0.4
66,856 + -2.0
66,857 - +1.0
66,857 - +0.7
67,421 + Psest_0066 0.85 +1.0
67,421 + Psest_0066 0.85 +0.0
67,421 + +2.7
67,422 - Psest_0066 0.86 +0.2
67,422 - Psest_0066 0.86 +0.5
67,699 - Psest_0067 0.20 +0.1

Or see this region's nucleotide sequence