Experiment: D-Cycloserine 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3087 and Psest_3088 overlap by 16 nucleotides Psest_3088 and Psest_3089 are separated by 127 nucleotides Psest_3089 and Psest_3090 overlap by 8 nucleotides
Psest_3087: Psest_3087 - Transcriptional regulator, at 3,267,317 to 3,268,273
_3087
Psest_3088: Psest_3088 - Protein of unknown function (DUF2905)., at 3,268,258 to 3,268,464
_3088
Psest_3089: Psest_3089 - pyridoxine 5'-phosphate synthase, at 3,268,592 to 3,269,338
_3089
Psest_3090: Psest_3090 - DNA repair protein RecO, at 3,269,331 to 3,270,020
_3090
Position (kb)
3268
3269 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3267.280 kb on + strand at 3267.280 kb on + strand at 3267.280 kb on + strand at 3267.281 kb on - strand at 3267.437 kb on + strand, within Psest_3087 at 3267.437 kb on + strand, within Psest_3087 at 3267.532 kb on + strand, within Psest_3087 at 3267.661 kb on + strand, within Psest_3087 at 3267.667 kb on + strand, within Psest_3087 at 3267.837 kb on + strand, within Psest_3087 at 3267.837 kb on + strand, within Psest_3087 at 3267.838 kb on - strand, within Psest_3087 at 3267.841 kb on + strand, within Psest_3087 at 3267.841 kb on + strand, within Psest_3087 at 3267.841 kb on + strand, within Psest_3087 at 3267.983 kb on - strand, within Psest_3087 at 3268.038 kb on + strand, within Psest_3087 at 3268.293 kb on - strand, within Psest_3088 at 3268.318 kb on + strand, within Psest_3088 at 3268.319 kb on - strand, within Psest_3088 at 3268.319 kb on - strand, within Psest_3088 at 3268.319 kb on - strand, within Psest_3088 at 3268.319 kb on - strand, within Psest_3088 at 3268.475 kb on - strand at 3268.564 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.032 mg/ml remove 3,267,280 + +0.3 3,267,280 + -1.9 3,267,280 + +0.6 3,267,281 - +0.4 3,267,437 + Psest_3087 0.13 -0.7 3,267,437 + Psest_3087 0.13 -0.7 3,267,532 + Psest_3087 0.22 -1.7 3,267,661 + Psest_3087 0.36 +2.6 3,267,667 + Psest_3087 0.37 +0.1 3,267,837 + Psest_3087 0.54 -1.1 3,267,837 + Psest_3087 0.54 -1.4 3,267,838 - Psest_3087 0.54 -0.3 3,267,841 + Psest_3087 0.55 +0.4 3,267,841 + Psest_3087 0.55 -0.1 3,267,841 + Psest_3087 0.55 +0.2 3,267,983 - Psest_3087 0.70 -0.9 3,268,038 + Psest_3087 0.75 +1.5 3,268,293 - Psest_3088 0.17 -2.6 3,268,318 + Psest_3088 0.29 -0.6 3,268,319 - Psest_3088 0.29 +0.2 3,268,319 - Psest_3088 0.29 -1.3 3,268,319 - Psest_3088 0.29 -0.7 3,268,319 - Psest_3088 0.29 -0.1 3,268,475 - +2.4 3,268,564 - +0.2
Or see this region's nucleotide sequence