Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2218

Experiment: D-Cycloserine 0.032 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2217 and Psest_2218 are separated by 13 nucleotidesPsest_2218 and Psest_2219 overlap by 4 nucleotidesPsest_2219 and Psest_2220 overlap by 1 nucleotides Psest_2217: Psest_2217 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes, at 2,366,006 to 2,367,154 _2217 Psest_2218: Psest_2218 - Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit, at 2,367,168 to 2,368,142 _2218 Psest_2219: Psest_2219 - pyruvate dehydrogenase E1 component, alpha subunit, at 2,368,139 to 2,369,131 _2219 Psest_2220: Psest_2220 - Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases, at 2,369,131 to 2,370,888 _2220 Position (kb) 2367 2368 2369Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2366.174 kb on + strand, within Psest_2217at 2366.174 kb on + strand, within Psest_2217at 2366.174 kb on + strand, within Psest_2217at 2366.175 kb on - strand, within Psest_2217at 2366.197 kb on - strand, within Psest_2217at 2366.451 kb on + strand, within Psest_2217at 2366.507 kb on + strand, within Psest_2217at 2366.508 kb on - strand, within Psest_2217at 2366.508 kb on - strand, within Psest_2217at 2366.550 kb on + strand, within Psest_2217at 2366.550 kb on + strand, within Psest_2217at 2366.601 kb on + strand, within Psest_2217at 2366.601 kb on + strand, within Psest_2217at 2366.602 kb on - strand, within Psest_2217at 2366.602 kb on - strand, within Psest_2217at 2367.170 kb on - strandat 2367.610 kb on + strand, within Psest_2218at 2367.610 kb on + strand, within Psest_2218at 2367.611 kb on - strand, within Psest_2218at 2368.027 kb on + strand, within Psest_2218at 2368.124 kb on - strandat 2368.161 kb on + strandat 2368.161 kb on + strandat 2368.161 kb on + strandat 2368.162 kb on - strandat 2368.162 kb on - strandat 2368.308 kb on + strand, within Psest_2219at 2368.308 kb on + strand, within Psest_2219at 2368.308 kb on + strand, within Psest_2219at 2368.309 kb on - strand, within Psest_2219at 2368.362 kb on + strand, within Psest_2219at 2368.402 kb on - strand, within Psest_2219at 2368.402 kb on - strand, within Psest_2219at 2368.422 kb on + strand, within Psest_2219at 2368.857 kb on + strand, within Psest_2219at 2368.858 kb on - strand, within Psest_2219at 2368.858 kb on - strand, within Psest_2219at 2368.920 kb on + strand, within Psest_2219at 2368.968 kb on - strand, within Psest_2219at 2368.992 kb on + strand, within Psest_2219at 2368.992 kb on + strand, within Psest_2219at 2368.992 kb on + strand, within Psest_2219at 2368.992 kb on + strand, within Psest_2219at 2368.992 kb on + strand, within Psest_2219at 2368.992 kb on + strand, within Psest_2219at 2368.992 kb on + strand, within Psest_2219at 2368.993 kb on - strand, within Psest_2219at 2368.993 kb on - strand, within Psest_2219at 2368.993 kb on - strand, within Psest_2219at 2368.993 kb on - strand, within Psest_2219at 2368.993 kb on - strand, within Psest_2219at 2368.993 kb on - strand, within Psest_2219at 2368.993 kb on - strand, within Psest_2219

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.032 mg/ml
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2,366,174 + Psest_2217 0.15 +1.1
2,366,174 + Psest_2217 0.15 +0.4
2,366,174 + Psest_2217 0.15 -2.3
2,366,175 - Psest_2217 0.15 +0.3
2,366,197 - Psest_2217 0.17 -1.4
2,366,451 + Psest_2217 0.39 -1.0
2,366,507 + Psest_2217 0.44 +0.7
2,366,508 - Psest_2217 0.44 +0.5
2,366,508 - Psest_2217 0.44 -1.0
2,366,550 + Psest_2217 0.47 +0.5
2,366,550 + Psest_2217 0.47 +0.3
2,366,601 + Psest_2217 0.52 +0.0
2,366,601 + Psest_2217 0.52 +1.7
2,366,602 - Psest_2217 0.52 +0.7
2,366,602 - Psest_2217 0.52 -1.4
2,367,170 - +1.7
2,367,610 + Psest_2218 0.45 +2.9
2,367,610 + Psest_2218 0.45 +2.1
2,367,611 - Psest_2218 0.45 +0.1
2,368,027 + Psest_2218 0.88 -0.5
2,368,124 - -0.6
2,368,161 + -1.1
2,368,161 + -1.6
2,368,161 + +3.0
2,368,162 - -0.7
2,368,162 - -1.5
2,368,308 + Psest_2219 0.17 +0.4
2,368,308 + Psest_2219 0.17 +0.6
2,368,308 + Psest_2219 0.17 +1.7
2,368,309 - Psest_2219 0.17 +0.6
2,368,362 + Psest_2219 0.22 +0.6
2,368,402 - Psest_2219 0.26 -1.1
2,368,402 - Psest_2219 0.26 -0.9
2,368,422 + Psest_2219 0.28 +0.0
2,368,857 + Psest_2219 0.72 -2.0
2,368,858 - Psest_2219 0.72 +0.1
2,368,858 - Psest_2219 0.72 -1.0
2,368,920 + Psest_2219 0.79 +1.1
2,368,968 - Psest_2219 0.83 +0.1
2,368,992 + Psest_2219 0.86 -1.2
2,368,992 + Psest_2219 0.86 -0.6
2,368,992 + Psest_2219 0.86 -0.9
2,368,992 + Psest_2219 0.86 +0.4
2,368,992 + Psest_2219 0.86 -0.4
2,368,992 + Psest_2219 0.86 -1.9
2,368,992 + Psest_2219 0.86 +0.9
2,368,993 - Psest_2219 0.86 -0.7
2,368,993 - Psest_2219 0.86 -1.1
2,368,993 - Psest_2219 0.86 -1.1
2,368,993 - Psest_2219 0.86 +1.8
2,368,993 - Psest_2219 0.86 -0.4
2,368,993 - Psest_2219 0.86 -0.4
2,368,993 - Psest_2219 0.86 -0.8

Or see this region's nucleotide sequence