Experiment: D-Cycloserine 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1674 and Psest_1675 are separated by 45 nucleotides Psest_1675 and Psest_1676 overlap by 4 nucleotides Psest_1676 and Psest_1677 are separated by 1 nucleotides Psest_1677 and Psest_1678 overlap by 4 nucleotides
Psest_1674: Psest_1674 - tetraacyldisaccharide 4'-kinase, at 1,782,612 to 1,783,616
_1674
Psest_1675: Psest_1675 - Uncharacterized conserved protein, at 1,783,662 to 1,783,847
_1675
Psest_1676: Psest_1676 - 3-deoxy-D-manno-octulosonate cytidylyltransferase, at 1,783,844 to 1,784,608
_1676
Psest_1677: Psest_1677 - Protein-tyrosine-phosphatase, at 1,784,610 to 1,785,077
_1677
Psest_1678: Psest_1678 - UDP-N-acetylenolpyruvoylglucosamine reductase, at 1,785,074 to 1,786,093
_1678
Position (kb) 1784
1785
1786 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1783.614 kb on + strand at 1783.614 kb on + strand at 1783.614 kb on + strand at 1783.730 kb on + strand, within Psest_1675 at 1784.639 kb on + strand at 1784.971 kb on + strand, within Psest_1677 at 1784.985 kb on + strand, within Psest_1677 at 1784.985 kb on + strand, within Psest_1677 at 1785.061 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.032 mg/ml remove 1,783,614 + -2.0 1,783,614 + +0.2 1,783,614 + -2.7 1,783,730 + Psest_1675 0.37 +1.4 1,784,639 + +2.3 1,784,971 + Psest_1677 0.77 +0.6 1,784,985 + Psest_1677 0.80 +1.8 1,784,985 + Psest_1677 0.80 -1.1 1,785,061 + +1.0
Or see this region's nucleotide sequence