Experiment: D-Cycloserine 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1639 and Psest_1640 are separated by 105 nucleotides Psest_1640 and Psest_1641 are separated by 160 nucleotides
Psest_1639: Psest_1639 - 23S rRNA (uracil-5-)-methyltransferase RumA, at 1,741,857 to 1,743,209
_1639
Psest_1640: Psest_1640 - (p)ppGpp synthetase, RelA/SpoT family, at 1,743,315 to 1,745,558
_1640
Psest_1641: Psest_1641 - MazG family protein, at 1,745,719 to 1,746,549
_1641
Position (kb)
1743
1744
1745
1746 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1742.405 kb on - strand, within Psest_1639 at 1742.482 kb on + strand, within Psest_1639 at 1742.482 kb on + strand, within Psest_1639 at 1742.482 kb on + strand, within Psest_1639 at 1742.482 kb on + strand, within Psest_1639 at 1743.066 kb on + strand, within Psest_1639 at 1743.066 kb on + strand, within Psest_1639 at 1743.066 kb on + strand, within Psest_1639 at 1743.067 kb on - strand, within Psest_1639 at 1743.067 kb on - strand, within Psest_1639 at 1743.067 kb on - strand, within Psest_1639 at 1743.067 kb on - strand, within Psest_1639 at 1743.067 kb on - strand, within Psest_1639 at 1743.067 kb on - strand, within Psest_1639 at 1743.067 kb on - strand, within Psest_1639 at 1743.155 kb on + strand at 1743.156 kb on - strand at 1743.312 kb on + strand at 1743.312 kb on + strand at 1743.312 kb on + strand at 1743.313 kb on - strand at 1743.320 kb on - strand at 1743.320 kb on - strand at 1743.359 kb on + strand at 1743.360 kb on - strand at 1743.927 kb on - strand, within Psest_1640 at 1743.978 kb on + strand, within Psest_1640 at 1743.978 kb on + strand, within Psest_1640 at 1743.979 kb on - strand, within Psest_1640 at 1743.979 kb on - strand, within Psest_1640 at 1743.979 kb on - strand, within Psest_1640 at 1744.029 kb on + strand, within Psest_1640 at 1744.029 kb on + strand, within Psest_1640 at 1744.029 kb on + strand, within Psest_1640 at 1744.029 kb on + strand, within Psest_1640 at 1744.029 kb on + strand, within Psest_1640 at 1744.029 kb on + strand, within Psest_1640 at 1744.029 kb on + strand, within Psest_1640 at 1744.029 kb on + strand, within Psest_1640 at 1744.030 kb on - strand, within Psest_1640 at 1744.030 kb on - strand, within Psest_1640 at 1744.030 kb on - strand, within Psest_1640 at 1744.030 kb on - strand, within Psest_1640 at 1744.030 kb on - strand, within Psest_1640 at 1744.030 kb on - strand, within Psest_1640 at 1744.030 kb on - strand, within Psest_1640 at 1744.116 kb on + strand, within Psest_1640 at 1744.117 kb on - strand, within Psest_1640 at 1744.167 kb on + strand, within Psest_1640 at 1744.168 kb on - strand, within Psest_1640 at 1744.168 kb on - strand, within Psest_1640 at 1744.215 kb on + strand, within Psest_1640 at 1744.275 kb on + strand, within Psest_1640 at 1744.275 kb on + strand, within Psest_1640 at 1744.275 kb on + strand, within Psest_1640 at 1744.275 kb on + strand, within Psest_1640 at 1744.275 kb on + strand, within Psest_1640 at 1744.275 kb on + strand, within Psest_1640 at 1744.275 kb on + strand, within Psest_1640 at 1744.276 kb on - strand, within Psest_1640 at 1744.276 kb on - strand, within Psest_1640 at 1744.416 kb on + strand, within Psest_1640 at 1744.563 kb on + strand, within Psest_1640 at 1744.563 kb on + strand, within Psest_1640 at 1744.564 kb on - strand, within Psest_1640 at 1744.655 kb on + strand, within Psest_1640 at 1744.656 kb on - strand, within Psest_1640 at 1744.656 kb on - strand, within Psest_1640 at 1744.701 kb on + strand, within Psest_1640 at 1744.702 kb on - strand, within Psest_1640 at 1744.702 kb on - strand, within Psest_1640 at 1744.702 kb on - strand, within Psest_1640 at 1744.702 kb on - strand, within Psest_1640 at 1744.702 kb on - strand, within Psest_1640 at 1744.737 kb on - strand, within Psest_1640 at 1744.737 kb on - strand, within Psest_1640 at 1744.788 kb on + strand, within Psest_1640 at 1744.788 kb on + strand, within Psest_1640 at 1744.789 kb on - strand, within Psest_1640 at 1744.789 kb on - strand, within Psest_1640 at 1745.193 kb on + strand, within Psest_1640 at 1745.193 kb on + strand, within Psest_1640 at 1745.219 kb on + strand, within Psest_1640 at 1745.369 kb on + strand at 1745.369 kb on + strand at 1745.369 kb on + strand at 1745.369 kb on + strand at 1745.556 kb on + strand at 1745.673 kb on + strand at 1745.673 kb on + strand at 1745.673 kb on + strand at 1745.722 kb on + strand at 1745.722 kb on + strand at 1745.722 kb on + strand at 1745.723 kb on - strand at 1745.723 kb on - strand at 1745.723 kb on - strand at 1745.804 kb on - strand, within Psest_1641 at 1745.839 kb on + strand, within Psest_1641 at 1745.840 kb on - strand, within Psest_1641 at 1745.840 kb on - strand, within Psest_1641 at 1745.840 kb on - strand, within Psest_1641 at 1745.920 kb on + strand, within Psest_1641 at 1745.920 kb on + strand, within Psest_1641 at 1745.921 kb on - strand, within Psest_1641 at 1745.923 kb on + strand, within Psest_1641 at 1745.924 kb on - strand, within Psest_1641 at 1746.034 kb on + strand, within Psest_1641 at 1746.034 kb on + strand, within Psest_1641 at 1746.035 kb on - strand, within Psest_1641 at 1746.035 kb on - strand, within Psest_1641 at 1746.035 kb on - strand, within Psest_1641
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.032 mg/ml remove 1,742,405 - Psest_1639 0.41 -0.3 1,742,482 + Psest_1639 0.46 +0.8 1,742,482 + Psest_1639 0.46 +1.7 1,742,482 + Psest_1639 0.46 +0.1 1,742,482 + Psest_1639 0.46 -1.9 1,743,066 + Psest_1639 0.89 +1.2 1,743,066 + Psest_1639 0.89 +0.0 1,743,066 + Psest_1639 0.89 -1.8 1,743,067 - Psest_1639 0.89 +1.7 1,743,067 - Psest_1639 0.89 +2.0 1,743,067 - Psest_1639 0.89 +1.5 1,743,067 - Psest_1639 0.89 -0.1 1,743,067 - Psest_1639 0.89 +4.5 1,743,067 - Psest_1639 0.89 +3.2 1,743,067 - Psest_1639 0.89 +1.2 1,743,155 + -0.1 1,743,156 - +2.5 1,743,312 + +1.7 1,743,312 + +2.7 1,743,312 + +3.0 1,743,313 - +0.2 1,743,320 - +2.1 1,743,320 - -0.4 1,743,359 + +1.6 1,743,360 - +0.5 1,743,927 - Psest_1640 0.27 +2.0 1,743,978 + Psest_1640 0.30 -0.5 1,743,978 + Psest_1640 0.30 +2.1 1,743,979 - Psest_1640 0.30 +1.6 1,743,979 - Psest_1640 0.30 +2.1 1,743,979 - Psest_1640 0.30 +0.5 1,744,029 + Psest_1640 0.32 +2.4 1,744,029 + Psest_1640 0.32 +0.6 1,744,029 + Psest_1640 0.32 +1.1 1,744,029 + Psest_1640 0.32 +1.7 1,744,029 + Psest_1640 0.32 +2.5 1,744,029 + Psest_1640 0.32 +0.7 1,744,029 + Psest_1640 0.32 +4.0 1,744,029 + Psest_1640 0.32 +2.3 1,744,030 - Psest_1640 0.32 +0.8 1,744,030 - Psest_1640 0.32 +4.8 1,744,030 - Psest_1640 0.32 +1.3 1,744,030 - Psest_1640 0.32 +2.0 1,744,030 - Psest_1640 0.32 +0.7 1,744,030 - Psest_1640 0.32 +2.3 1,744,030 - Psest_1640 0.32 +0.8 1,744,116 + Psest_1640 0.36 +2.2 1,744,117 - Psest_1640 0.36 +2.5 1,744,167 + Psest_1640 0.38 +2.4 1,744,168 - Psest_1640 0.38 +1.7 1,744,168 - Psest_1640 0.38 +1.7 1,744,215 + Psest_1640 0.40 +1.5 1,744,275 + Psest_1640 0.43 +0.4 1,744,275 + Psest_1640 0.43 +0.9 1,744,275 + Psest_1640 0.43 +2.5 1,744,275 + Psest_1640 0.43 +0.1 1,744,275 + Psest_1640 0.43 -0.5 1,744,275 + Psest_1640 0.43 +1.7 1,744,275 + Psest_1640 0.43 +1.3 1,744,276 - Psest_1640 0.43 +1.8 1,744,276 - Psest_1640 0.43 +0.5 1,744,416 + Psest_1640 0.49 +2.6 1,744,563 + Psest_1640 0.56 +1.5 1,744,563 + Psest_1640 0.56 +1.4 1,744,564 - Psest_1640 0.56 +1.0 1,744,655 + Psest_1640 0.60 +0.7 1,744,656 - Psest_1640 0.60 +1.3 1,744,656 - Psest_1640 0.60 +3.2 1,744,701 + Psest_1640 0.62 +2.9 1,744,702 - Psest_1640 0.62 +1.7 1,744,702 - Psest_1640 0.62 +0.6 1,744,702 - Psest_1640 0.62 +1.4 1,744,702 - Psest_1640 0.62 +0.4 1,744,702 - Psest_1640 0.62 -0.5 1,744,737 - Psest_1640 0.63 +1.2 1,744,737 - Psest_1640 0.63 +2.4 1,744,788 + Psest_1640 0.66 +0.6 1,744,788 + Psest_1640 0.66 +0.2 1,744,789 - Psest_1640 0.66 +1.5 1,744,789 - Psest_1640 0.66 +0.4 1,745,193 + Psest_1640 0.84 +0.6 1,745,193 + Psest_1640 0.84 -0.6 1,745,219 + Psest_1640 0.85 +0.5 1,745,369 + -0.9 1,745,369 + +0.0 1,745,369 + +0.7 1,745,369 + -0.9 1,745,556 + -2.5 1,745,673 + -1.5 1,745,673 + -0.4 1,745,673 + +0.4 1,745,722 + +0.5 1,745,722 + -1.9 1,745,722 + +0.5 1,745,723 - +1.5 1,745,723 - -0.1 1,745,723 - +0.6 1,745,804 - Psest_1641 0.10 -1.1 1,745,839 + Psest_1641 0.14 -0.3 1,745,840 - Psest_1641 0.15 +0.1 1,745,840 - Psest_1641 0.15 +0.6 1,745,840 - Psest_1641 0.15 +0.5 1,745,920 + Psest_1641 0.24 -1.5 1,745,920 + Psest_1641 0.24 -1.4 1,745,921 - Psest_1641 0.24 -0.2 1,745,923 + Psest_1641 0.25 +0.6 1,745,924 - Psest_1641 0.25 +0.9 1,746,034 + Psest_1641 0.38 +0.8 1,746,034 + Psest_1641 0.38 +1.9 1,746,035 - Psest_1641 0.38 -0.3 1,746,035 - Psest_1641 0.38 -0.8 1,746,035 - Psest_1641 0.38 +1.7
Or see this region's nucleotide sequence