Experiment: D-Cycloserine 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0115 and Psest_0116 are separated by 16 nucleotides Psest_0116 and Psest_0117 overlap by 4 nucleotides Psest_0117 and Psest_0118 overlap by 4 nucleotides Psest_0118 and Psest_0119 are separated by 21 nucleotides Psest_0119 and Psest_0120 are separated by 213 nucleotides
Psest_0115: Psest_0115 - Predicted integral membrane protein, at 113,172 to 113,444
_0115
Psest_0116: Psest_0116 - Uncharacterized protein conserved in bacteria, at 113,461 to 114,288
_0116
Psest_0117: Psest_0117 - Uncharacterized protein conserved in bacteria (DUF2063)., at 114,285 to 115,076
_0117
Psest_0118: Psest_0118 - Predicted membrane protein, at 115,073 to 115,534
_0118
Psest_0119: Psest_0119 - hypothetical protein, at 115,556 to 115,762
_0119
Psest_0120: Psest_0120 - hypothetical protein, at 115,976 to 116,320
_0120
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 113.384 kb on + strand, within Psest_0115 at 113.448 kb on + strand at 113.449 kb on - strand at 113.484 kb on - strand at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.888 kb on - strand, within Psest_0116 at 113.888 kb on - strand, within Psest_0116 at 114.505 kb on - strand, within Psest_0117 at 114.505 kb on - strand, within Psest_0117 at 114.505 kb on - strand, within Psest_0117 at 114.547 kb on - strand, within Psest_0117 at 114.603 kb on + strand, within Psest_0117 at 114.604 kb on - strand, within Psest_0117 at 114.604 kb on - strand, within Psest_0117 at 114.777 kb on + strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 115.325 kb on + strand, within Psest_0118 at 115.325 kb on + strand, within Psest_0118 at 115.325 kb on + strand, within Psest_0118 at 115.325 kb on + strand, within Psest_0118 at 115.325 kb on + strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.326 kb on - strand, within Psest_0118 at 115.473 kb on + strand, within Psest_0118 at 115.474 kb on - strand, within Psest_0118 at 115.474 kb on - strand, within Psest_0118 at 115.474 kb on - strand, within Psest_0118 at 115.650 kb on + strand, within Psest_0119 at 115.650 kb on + strand, within Psest_0119 at 115.651 kb on - strand, within Psest_0119 at 115.651 kb on - strand, within Psest_0119 at 115.651 kb on - strand, within Psest_0119 at 115.834 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.907 kb on - strand at 115.933 kb on - strand at 115.933 kb on - strand at 115.933 kb on - strand at 115.933 kb on - strand at 115.933 kb on - strand at 115.985 kb on - strand at 115.985 kb on - strand at 115.988 kb on - strand at 115.991 kb on + strand at 116.026 kb on + strand, within Psest_0120 at 116.065 kb on - strand, within Psest_0120
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.032 mg/ml remove 113,384 + Psest_0115 0.78 -0.8 113,448 + +1.1 113,449 - -0.1 113,484 - +0.0 113,563 + Psest_0116 0.12 -0.9 113,563 + Psest_0116 0.12 +1.1 113,563 + Psest_0116 0.12 -0.4 113,563 + Psest_0116 0.12 +2.2 113,563 + Psest_0116 0.12 +1.6 113,563 + Psest_0116 0.12 +1.6 113,564 - Psest_0116 0.12 +2.1 113,564 - Psest_0116 0.12 +0.2 113,564 - Psest_0116 0.12 -0.0 113,564 - Psest_0116 0.12 +1.3 113,564 - Psest_0116 0.12 +1.0 113,564 - Psest_0116 0.12 +1.3 113,564 - Psest_0116 0.12 -0.7 113,564 - Psest_0116 0.12 +0.6 113,767 + Psest_0116 0.37 -1.2 113,767 + Psest_0116 0.37 -1.3 113,767 + Psest_0116 0.37 -0.7 113,767 + Psest_0116 0.37 -1.8 113,767 + Psest_0116 0.37 +0.4 113,767 + Psest_0116 0.37 -1.3 113,768 - Psest_0116 0.37 +1.7 113,768 - Psest_0116 0.37 -0.2 113,768 - Psest_0116 0.37 -0.8 113,768 - Psest_0116 0.37 +1.5 113,768 - Psest_0116 0.37 +3.6 113,768 - Psest_0116 0.37 -0.5 113,768 - Psest_0116 0.37 +0.0 113,888 - Psest_0116 0.52 -1.0 113,888 - Psest_0116 0.52 -1.0 114,505 - Psest_0117 0.28 +0.0 114,505 - Psest_0117 0.28 +0.7 114,505 - Psest_0117 0.28 -0.1 114,547 - Psest_0117 0.33 -0.0 114,603 + Psest_0117 0.40 -0.1 114,604 - Psest_0117 0.40 +0.5 114,604 - Psest_0117 0.40 +0.8 114,777 + Psest_0117 0.62 -1.0 114,778 - Psest_0117 0.62 -1.0 114,778 - Psest_0117 0.62 -1.8 114,778 - Psest_0117 0.62 +0.1 114,778 - Psest_0117 0.62 -1.3 114,778 - Psest_0117 0.62 +0.2 114,907 + Psest_0117 0.79 +0.2 114,907 + Psest_0117 0.79 -0.5 114,907 + Psest_0117 0.79 -2.5 114,907 + Psest_0117 0.79 +1.2 114,907 + Psest_0117 0.79 -0.7 114,908 - Psest_0117 0.79 -0.8 114,908 - Psest_0117 0.79 -0.7 114,908 - Psest_0117 0.79 +0.1 114,908 - Psest_0117 0.79 -2.9 114,908 - Psest_0117 0.79 -1.7 114,908 - Psest_0117 0.79 +0.7 115,325 + Psest_0118 0.55 +0.7 115,325 + Psest_0118 0.55 -2.1 115,325 + Psest_0118 0.55 -0.4 115,325 + Psest_0118 0.55 -2.0 115,325 + Psest_0118 0.55 +0.0 115,326 - Psest_0118 0.55 +1.3 115,326 - Psest_0118 0.55 -1.4 115,326 - Psest_0118 0.55 +2.2 115,326 - Psest_0118 0.55 +0.9 115,326 - Psest_0118 0.55 +1.2 115,326 - Psest_0118 0.55 -1.4 115,326 - Psest_0118 0.55 +1.7 115,326 - Psest_0118 0.55 -1.1 115,473 + Psest_0118 0.87 -0.3 115,474 - Psest_0118 0.87 -0.4 115,474 - Psest_0118 0.87 +2.6 115,474 - Psest_0118 0.87 -1.2 115,650 + Psest_0119 0.45 +2.2 115,650 + Psest_0119 0.45 +1.5 115,651 - Psest_0119 0.46 -2.9 115,651 - Psest_0119 0.46 -3.3 115,651 - Psest_0119 0.46 +1.7 115,834 - -2.9 115,907 - +0.8 115,907 - +0.1 115,907 - -0.7 115,907 - -2.0 115,907 - +1.3 115,907 - +1.3 115,907 - +0.2 115,907 - +2.5 115,933 - -0.7 115,933 - +0.8 115,933 - -0.6 115,933 - -0.3 115,933 - +0.7 115,985 - +0.1 115,985 - +2.3 115,988 - -0.1 115,991 + -0.8 116,026 + Psest_0120 0.14 +0.1 116,065 - Psest_0120 0.26 -0.1
Or see this region's nucleotide sequence