Experiment: D-Cycloserine 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1179 and Psest_1180 are separated by 32 nucleotides Psest_1180 and Psest_1181 are separated by 188 nucleotides
Psest_1179: Psest_1179 - 2-hydroxy-3-oxopropionate reductase, at 1,281,358 to 1,282,248
_1179
Psest_1180: Psest_1180 - hydroxypyruvate isomerase, at 1,282,281 to 1,283,063
_1180
Psest_1181: Psest_1181 - Glyoxylate carboligase (EC 4.1.1.47) (from data) , at 1,283,252 to 1,285,033
_1181
Position (kb)
1282
1283
1284 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1281.360 kb on - strand at 1281.902 kb on + strand, within Psest_1179 at 1281.902 kb on + strand, within Psest_1179 at 1282.231 kb on - strand at 1282.243 kb on - strand at 1282.243 kb on - strand at 1282.252 kb on + strand at 1282.252 kb on + strand at 1282.253 kb on - strand at 1282.253 kb on - strand at 1282.253 kb on - strand at 1282.342 kb on + strand at 1282.342 kb on + strand at 1282.342 kb on + strand at 1282.343 kb on - strand at 1282.343 kb on - strand at 1282.343 kb on - strand at 1282.402 kb on + strand, within Psest_1180 at 1282.403 kb on - strand, within Psest_1180 at 1282.403 kb on - strand, within Psest_1180 at 1282.403 kb on - strand, within Psest_1180 at 1282.403 kb on - strand, within Psest_1180 at 1282.403 kb on - strand, within Psest_1180 at 1282.458 kb on - strand, within Psest_1180 at 1282.526 kb on - strand, within Psest_1180 at 1282.534 kb on + strand, within Psest_1180 at 1282.534 kb on + strand, within Psest_1180 at 1282.534 kb on + strand, within Psest_1180 at 1282.535 kb on - strand, within Psest_1180 at 1282.787 kb on - strand, within Psest_1180 at 1282.945 kb on + strand, within Psest_1180 at 1282.964 kb on - strand, within Psest_1180 at 1283.084 kb on - strand at 1283.084 kb on - strand at 1283.084 kb on - strand at 1283.107 kb on + strand at 1283.107 kb on + strand at 1283.107 kb on + strand at 1283.107 kb on + strand at 1283.150 kb on + strand at 1283.150 kb on + strand at 1283.171 kb on + strand at 1283.171 kb on + strand at 1283.253 kb on + strand at 1283.254 kb on - strand at 1283.508 kb on + strand, within Psest_1181 at 1283.610 kb on + strand, within Psest_1181 at 1283.638 kb on - strand, within Psest_1181 at 1283.638 kb on - strand, within Psest_1181 at 1283.814 kb on + strand, within Psest_1181 at 1283.814 kb on + strand, within Psest_1181 at 1283.814 kb on + strand, within Psest_1181 at 1283.814 kb on + strand, within Psest_1181 at 1283.814 kb on + strand, within Psest_1181 at 1283.814 kb on + strand, within Psest_1181 at 1283.814 kb on + strand, within Psest_1181 at 1283.814 kb on + strand, within Psest_1181 at 1283.815 kb on - strand, within Psest_1181 at 1283.869 kb on - strand, within Psest_1181 at 1283.869 kb on - strand, within Psest_1181 at 1283.907 kb on + strand, within Psest_1181 at 1283.908 kb on - strand, within Psest_1181 at 1283.908 kb on - strand, within Psest_1181
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.032 mg/ml remove 1,281,360 - +1.1 1,281,902 + Psest_1179 0.61 +1.3 1,281,902 + Psest_1179 0.61 +1.3 1,282,231 - -0.5 1,282,243 - -1.1 1,282,243 - +1.3 1,282,252 + +1.7 1,282,252 + -0.6 1,282,253 - +1.4 1,282,253 - +0.0 1,282,253 - -0.2 1,282,342 + +0.1 1,282,342 + -0.9 1,282,342 + -0.4 1,282,343 - +1.9 1,282,343 - +1.5 1,282,343 - +1.4 1,282,402 + Psest_1180 0.15 -0.4 1,282,403 - Psest_1180 0.16 +1.6 1,282,403 - Psest_1180 0.16 +0.5 1,282,403 - Psest_1180 0.16 +0.4 1,282,403 - Psest_1180 0.16 +3.6 1,282,403 - Psest_1180 0.16 +2.4 1,282,458 - Psest_1180 0.23 +0.1 1,282,526 - Psest_1180 0.31 -0.4 1,282,534 + Psest_1180 0.32 +1.4 1,282,534 + Psest_1180 0.32 -2.8 1,282,534 + Psest_1180 0.32 -2.3 1,282,535 - Psest_1180 0.32 +1.5 1,282,787 - Psest_1180 0.65 +0.8 1,282,945 + Psest_1180 0.85 +0.8 1,282,964 - Psest_1180 0.87 -0.9 1,283,084 - +2.2 1,283,084 - +0.7 1,283,084 - +2.0 1,283,107 + +0.6 1,283,107 + -0.6 1,283,107 + -0.5 1,283,107 + +1.2 1,283,150 + +1.1 1,283,150 + +1.0 1,283,171 + +2.1 1,283,171 + +0.2 1,283,253 + +0.7 1,283,254 - -2.9 1,283,508 + Psest_1181 0.14 +0.7 1,283,610 + Psest_1181 0.20 +0.3 1,283,638 - Psest_1181 0.22 +2.1 1,283,638 - Psest_1181 0.22 +2.2 1,283,814 + Psest_1181 0.32 +0.8 1,283,814 + Psest_1181 0.32 +0.9 1,283,814 + Psest_1181 0.32 +0.3 1,283,814 + Psest_1181 0.32 +1.5 1,283,814 + Psest_1181 0.32 -1.3 1,283,814 + Psest_1181 0.32 -0.1 1,283,814 + Psest_1181 0.32 +0.9 1,283,814 + Psest_1181 0.32 +0.1 1,283,815 - Psest_1181 0.32 -1.1 1,283,869 - Psest_1181 0.35 +0.6 1,283,869 - Psest_1181 0.35 +4.0 1,283,907 + Psest_1181 0.37 -1.1 1,283,908 - Psest_1181 0.37 +0.8 1,283,908 - Psest_1181 0.37 +0.6
Or see this region's nucleotide sequence