Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2302

Experiment: A22 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2301 and Psest_2302 are separated by 58 nucleotidesPsest_2302 and Psest_2303 overlap by 4 nucleotides Psest_2301: Psest_2301 - 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase, at 2,451,146 to 2,452,000 _2301 Psest_2302: Psest_2302 - cysteinyl-tRNA synthetase, at 2,452,059 to 2,453,447 _2302 Psest_2303: Psest_2303 - glutaminyl-tRNA synthetase, at 2,453,444 to 2,455,174 _2303 Position (kb) 2452 2453 2454Strain fitness (log2 ratio) -1 0 1at 2452.048 kb on + strandat 2452.048 kb on + strandat 2452.049 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.005 mg/ml
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2,452,048 + +0.7
2,452,048 + -0.8
2,452,049 - +0.3

Or see this region's nucleotide sequence