Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0399 and Psest_0400 are separated by 105 nucleotides Psest_0400 and Psest_0401 are separated by 158 nucleotides
Psest_0399: Psest_0399 - Acyl-CoA dehydrogenases, at 432,761 to 434,575
_0399
Psest_0400: Psest_0400 - Geranylgeranyl pyrophosphate synthase, at 434,681 to 435,571
_0400
Psest_0401: Psest_0401 - isopentenyl-diphosphate delta-isomerase, type 2, at 435,730 to 436,770
_0401
Position (kb) 434
435
436 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 433.710 kb on + strand, within Psest_0399 at 433.710 kb on + strand, within Psest_0399 at 433.710 kb on + strand, within Psest_0399 at 433.710 kb on + strand, within Psest_0399 at 433.710 kb on + strand, within Psest_0399 at 433.711 kb on - strand, within Psest_0399 at 433.711 kb on - strand, within Psest_0399 at 433.711 kb on - strand, within Psest_0399 at 433.711 kb on - strand, within Psest_0399 at 433.711 kb on - strand, within Psest_0399 at 434.421 kb on + strand at 434.422 kb on - strand at 434.422 kb on - strand at 434.422 kb on - strand at 434.529 kb on + strand at 434.529 kb on + strand at 434.606 kb on + strand at 434.606 kb on + strand at 434.630 kb on + strand at 435.086 kb on + strand, within Psest_0400 at 435.086 kb on + strand, within Psest_0400 at 435.086 kb on + strand, within Psest_0400 at 435.086 kb on + strand, within Psest_0400 at 435.086 kb on + strand, within Psest_0400 at 435.087 kb on - strand, within Psest_0400 at 435.377 kb on + strand, within Psest_0400 at 435.377 kb on + strand, within Psest_0400 at 435.386 kb on + strand, within Psest_0400 at 435.386 kb on + strand, within Psest_0400 at 435.454 kb on + strand, within Psest_0400 at 435.455 kb on - strand, within Psest_0400 at 435.625 kb on - strand at 435.640 kb on + strand at 435.641 kb on - strand at 435.675 kb on + strand at 436.223 kb on + strand, within Psest_0401
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 433,710 + Psest_0399 0.52 -1.3 433,710 + Psest_0399 0.52 +1.7 433,710 + Psest_0399 0.52 -1.1 433,710 + Psest_0399 0.52 +0.1 433,710 + Psest_0399 0.52 +1.3 433,711 - Psest_0399 0.52 -1.3 433,711 - Psest_0399 0.52 -0.5 433,711 - Psest_0399 0.52 +0.3 433,711 - Psest_0399 0.52 +0.3 433,711 - Psest_0399 0.52 +0.1 434,421 + -0.1 434,422 - +1.8 434,422 - -0.5 434,422 - +0.7 434,529 + -0.0 434,529 + -0.3 434,606 + -0.5 434,606 + -0.8 434,630 + +0.4 435,086 + Psest_0400 0.45 -0.8 435,086 + Psest_0400 0.45 -1.1 435,086 + Psest_0400 0.45 +0.7 435,086 + Psest_0400 0.45 -0.4 435,086 + Psest_0400 0.45 -1.4 435,087 - Psest_0400 0.46 +0.3 435,377 + Psest_0400 0.78 +0.3 435,377 + Psest_0400 0.78 -0.0 435,386 + Psest_0400 0.79 -1.8 435,386 + Psest_0400 0.79 -0.5 435,454 + Psest_0400 0.87 -1.7 435,455 - Psest_0400 0.87 +1.7 435,625 - +0.1 435,640 + -2.9 435,641 - +0.3 435,675 + -1.1 436,223 + Psest_0401 0.47 -1.3
Or see this region's nucleotide sequence