Experiment: A22 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0186 and Psest_0187 are separated by 6 nucleotides Psest_0187 and Psest_0188 overlap by 4 nucleotides Psest_0188 and Psest_0189 are separated by 112 nucleotides Psest_0189 and Psest_0190 are separated by 2 nucleotides
Psest_0186: Psest_0186 - Protein-disulfide isomerase, at 181,742 to 182,371
_0186
Psest_0187: Psest_0187 - Metal-dependent hydrolase, at 182,378 to 183,247
_0187
Psest_0188: Psest_0188 - diguanylate cyclase (GGDEF) domain, at 183,244 to 185,142
_0188
Psest_0189: Psest_0189 - Negative regulator of beta-lactamase expression, at 185,255 to 186,043
_0189
Psest_0190: Psest_0190 - Uncharacterized conserved protein, at 186,046 to 186,450
_0190
Position (kb) 183
184
185
186 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.534 kb on + strand, within Psest_0187 at 182.534 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.619 kb on - strand, within Psest_0187 at 182.721 kb on - strand, within Psest_0187 at 182.933 kb on + strand, within Psest_0187 at 182.990 kb on - strand, within Psest_0187 at 183.277 kb on + strand at 183.278 kb on - strand at 183.341 kb on + strand at 183.488 kb on + strand, within Psest_0188 at 183.488 kb on + strand, within Psest_0188 at 183.601 kb on - strand, within Psest_0188 at 183.601 kb on - strand, within Psest_0188 at 184.069 kb on + strand, within Psest_0188 at 184.225 kb on + strand, within Psest_0188 at 184.226 kb on - strand, within Psest_0188 at 184.306 kb on + strand, within Psest_0188 at 184.307 kb on - strand, within Psest_0188 at 184.524 kb on + strand, within Psest_0188 at 184.529 kb on - strand, within Psest_0188 at 184.529 kb on - strand, within Psest_0188 at 185.178 kb on - strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.442 kb on - strand, within Psest_0189 at 185.442 kb on - strand, within Psest_0189 at 185.864 kb on + strand, within Psest_0189 at 185.961 kb on - strand, within Psest_0189 at 186.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.002 mg/ml remove 182,282 + Psest_0186 0.86 +0.2 182,282 + Psest_0186 0.86 -2.5 182,282 + Psest_0186 0.86 +0.3 182,282 + Psest_0186 0.86 -0.8 182,282 + Psest_0186 0.86 -2.0 182,534 + Psest_0187 0.18 -0.0 182,534 + Psest_0187 0.18 -0.8 182,618 + Psest_0187 0.28 -0.3 182,618 + Psest_0187 0.28 +0.6 182,618 + Psest_0187 0.28 -1.6 182,618 + Psest_0187 0.28 -1.6 182,619 - Psest_0187 0.28 -1.7 182,721 - Psest_0187 0.39 -1.1 182,933 + Psest_0187 0.64 -1.1 182,990 - Psest_0187 0.70 -1.9 183,277 + -0.4 183,278 - +0.0 183,341 + +1.2 183,488 + Psest_0188 0.13 -0.3 183,488 + Psest_0188 0.13 -1.2 183,601 - Psest_0188 0.19 +0.7 183,601 - Psest_0188 0.19 -2.1 184,069 + Psest_0188 0.43 +0.1 184,225 + Psest_0188 0.52 -2.0 184,226 - Psest_0188 0.52 -1.9 184,306 + Psest_0188 0.56 +1.8 184,307 - Psest_0188 0.56 -1.0 184,524 + Psest_0188 0.67 -0.2 184,529 - Psest_0188 0.68 +2.1 184,529 - Psest_0188 0.68 +0.0 185,178 - -0.9 185,332 + +1.2 185,332 + -0.7 185,332 + -1.4 185,332 + +1.1 185,332 + +1.2 185,332 + -0.8 185,333 - -0.6 185,333 - -2.8 185,333 - -0.4 185,333 - -0.8 185,333 - +0.5 185,333 - +0.7 185,378 + Psest_0189 0.16 -1.2 185,378 + Psest_0189 0.16 -0.7 185,378 + Psest_0189 0.16 -0.3 185,378 + Psest_0189 0.16 -0.9 185,378 + Psest_0189 0.16 -1.6 185,379 - Psest_0189 0.16 +0.8 185,379 - Psest_0189 0.16 -0.2 185,379 - Psest_0189 0.16 -0.1 185,379 - Psest_0189 0.16 -1.0 185,379 - Psest_0189 0.16 -2.0 185,379 - Psest_0189 0.16 +0.5 185,379 - Psest_0189 0.16 -0.4 185,379 - Psest_0189 0.16 +2.8 185,379 - Psest_0189 0.16 +1.1 185,442 - Psest_0189 0.24 +1.6 185,442 - Psest_0189 0.24 -0.9 185,864 + Psest_0189 0.77 +1.2 185,961 - Psest_0189 0.89 +0.0 186,051 - -0.5
Or see this region's nucleotide sequence