Experiment: Vancomycin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2245 and Psest_2246 are separated by 18 nucleotides Psest_2246 and Psest_2247 overlap by 1 nucleotides Psest_2247 and Psest_2248 are separated by 13 nucleotides Psest_2248 and Psest_2249 are separated by 10 nucleotides Psest_2249 and Psest_2250 are separated by 196 nucleotides
Psest_2245: Psest_2245 - Predicted protein-tyrosine phosphatase, at 2,396,514 to 2,397,008
_2245
Psest_2246: Psest_2246 - arsenical resistance protein ArsH, at 2,397,027 to 2,397,740
_2246
Psest_2247: Psest_2247 - Protein-tyrosine-phosphatase, at 2,397,740 to 2,398,240
_2247
Psest_2248: Psest_2248 - Predicted transcriptional regulators, at 2,398,254 to 2,398,610
_2248
Psest_2249: Psest_2249 - Protein-tyrosine-phosphatase, at 2,398,621 to 2,399,034
_2249
Psest_2250: Psest_2250 - Predicted flavoprotein involved in K+ transport, at 2,399,231 to 2,400,313
_2250
Position (kb)
2397
2398
2399 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2396.980 kb on - strand at 2396.995 kb on + strand at 2396.995 kb on + strand at 2396.995 kb on + strand at 2396.995 kb on + strand at 2396.996 kb on - strand at 2396.996 kb on - strand at 2397.103 kb on + strand, within Psest_2246 at 2397.173 kb on - strand, within Psest_2246 at 2397.176 kb on - strand, within Psest_2246 at 2397.176 kb on - strand, within Psest_2246 at 2397.607 kb on + strand, within Psest_2246 at 2397.607 kb on + strand, within Psest_2246 at 2397.631 kb on + strand, within Psest_2246 at 2397.632 kb on - strand, within Psest_2246 at 2397.741 kb on + strand at 2397.742 kb on - strand at 2397.755 kb on + strand at 2397.756 kb on - strand at 2397.756 kb on - strand at 2397.756 kb on - strand at 2397.858 kb on - strand, within Psest_2247 at 2397.896 kb on + strand, within Psest_2247 at 2397.897 kb on - strand, within Psest_2247 at 2398.058 kb on - strand, within Psest_2247 at 2398.058 kb on - strand, within Psest_2247 at 2398.059 kb on + strand, within Psest_2247 at 2398.059 kb on + strand, within Psest_2247 at 2398.059 kb on + strand, within Psest_2247 at 2398.059 kb on + strand, within Psest_2247 at 2398.059 kb on + strand, within Psest_2247 at 2398.059 kb on + strand, within Psest_2247 at 2398.059 kb on + strand, within Psest_2247 at 2398.060 kb on - strand, within Psest_2247 at 2398.060 kb on - strand, within Psest_2247 at 2398.060 kb on - strand, within Psest_2247 at 2398.060 kb on - strand, within Psest_2247 at 2398.060 kb on - strand, within Psest_2247 at 2398.060 kb on - strand, within Psest_2247 at 2398.060 kb on - strand, within Psest_2247 at 2398.060 kb on - strand, within Psest_2247 at 2398.293 kb on - strand, within Psest_2248 at 2398.293 kb on - strand, within Psest_2248 at 2398.400 kb on - strand, within Psest_2248 at 2398.581 kb on + strand at 2398.581 kb on + strand at 2398.589 kb on + strand at 2398.589 kb on + strand at 2398.589 kb on + strand at 2398.590 kb on - strand at 2398.614 kb on + strand at 2398.615 kb on - strand at 2398.615 kb on - strand at 2398.666 kb on + strand, within Psest_2249 at 2398.666 kb on + strand, within Psest_2249 at 2398.666 kb on + strand, within Psest_2249 at 2398.666 kb on + strand, within Psest_2249 at 2398.666 kb on + strand, within Psest_2249 at 2398.667 kb on - strand, within Psest_2249 at 2398.667 kb on - strand, within Psest_2249 at 2398.667 kb on - strand, within Psest_2249 at 2398.667 kb on - strand, within Psest_2249 at 2398.667 kb on - strand, within Psest_2249 at 2398.667 kb on - strand, within Psest_2249 at 2398.739 kb on + strand, within Psest_2249 at 2398.739 kb on + strand, within Psest_2249 at 2398.740 kb on - strand, within Psest_2249 at 2398.782 kb on + strand, within Psest_2249 at 2398.782 kb on + strand, within Psest_2249 at 2398.782 kb on + strand, within Psest_2249 at 2398.783 kb on - strand, within Psest_2249 at 2398.783 kb on - strand, within Psest_2249 at 2398.783 kb on - strand, within Psest_2249 at 2398.783 kb on - strand, within Psest_2249 at 2398.894 kb on + strand, within Psest_2249 at 2398.894 kb on + strand, within Psest_2249 at 2398.894 kb on + strand, within Psest_2249 at 2398.895 kb on - strand, within Psest_2249 at 2399.027 kb on + strand at 2399.028 kb on - strand at 2399.068 kb on + strand at 2399.121 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.016 mg/ml remove 2,396,980 - +0.8 2,396,995 + +0.1 2,396,995 + -0.2 2,396,995 + -0.3 2,396,995 + +0.2 2,396,996 - +0.1 2,396,996 - +0.1 2,397,103 + Psest_2246 0.11 +0.8 2,397,173 - Psest_2246 0.20 +2.9 2,397,176 - Psest_2246 0.21 -0.7 2,397,176 - Psest_2246 0.21 +0.1 2,397,607 + Psest_2246 0.81 +1.4 2,397,607 + Psest_2246 0.81 +0.6 2,397,631 + Psest_2246 0.85 -0.3 2,397,632 - Psest_2246 0.85 -1.3 2,397,741 + +0.2 2,397,742 - -0.7 2,397,755 + -0.3 2,397,756 - -1.9 2,397,756 - +0.5 2,397,756 - +0.2 2,397,858 - Psest_2247 0.24 +0.2 2,397,896 + Psest_2247 0.31 +0.0 2,397,897 - Psest_2247 0.31 -0.2 2,398,058 - Psest_2247 0.63 -0.4 2,398,058 - Psest_2247 0.63 +0.6 2,398,059 + Psest_2247 0.64 -1.0 2,398,059 + Psest_2247 0.64 +1.8 2,398,059 + Psest_2247 0.64 -0.7 2,398,059 + Psest_2247 0.64 -0.4 2,398,059 + Psest_2247 0.64 +0.0 2,398,059 + Psest_2247 0.64 +1.7 2,398,059 + Psest_2247 0.64 -0.1 2,398,060 - Psest_2247 0.64 -1.2 2,398,060 - Psest_2247 0.64 -0.1 2,398,060 - Psest_2247 0.64 +2.0 2,398,060 - Psest_2247 0.64 +0.3 2,398,060 - Psest_2247 0.64 +1.3 2,398,060 - Psest_2247 0.64 +0.1 2,398,060 - Psest_2247 0.64 +1.5 2,398,060 - Psest_2247 0.64 +1.4 2,398,293 - Psest_2248 0.11 -0.3 2,398,293 - Psest_2248 0.11 +1.5 2,398,400 - Psest_2248 0.41 -1.4 2,398,581 + -0.0 2,398,581 + +0.7 2,398,589 + -0.8 2,398,589 + +0.0 2,398,589 + +1.0 2,398,590 - +0.6 2,398,614 + -1.2 2,398,615 - +0.1 2,398,615 - -0.9 2,398,666 + Psest_2249 0.11 -1.6 2,398,666 + Psest_2249 0.11 -0.1 2,398,666 + Psest_2249 0.11 +0.0 2,398,666 + Psest_2249 0.11 -0.4 2,398,666 + Psest_2249 0.11 -0.4 2,398,667 - Psest_2249 0.11 +0.1 2,398,667 - Psest_2249 0.11 -0.6 2,398,667 - Psest_2249 0.11 +0.2 2,398,667 - Psest_2249 0.11 +0.2 2,398,667 - Psest_2249 0.11 +0.5 2,398,667 - Psest_2249 0.11 +1.2 2,398,739 + Psest_2249 0.29 -3.0 2,398,739 + Psest_2249 0.29 -1.9 2,398,740 - Psest_2249 0.29 -0.8 2,398,782 + Psest_2249 0.39 -1.6 2,398,782 + Psest_2249 0.39 +0.8 2,398,782 + Psest_2249 0.39 -2.0 2,398,783 - Psest_2249 0.39 -0.5 2,398,783 - Psest_2249 0.39 +1.0 2,398,783 - Psest_2249 0.39 -0.5 2,398,783 - Psest_2249 0.39 +1.3 2,398,894 + Psest_2249 0.66 -1.4 2,398,894 + Psest_2249 0.66 -0.1 2,398,894 + Psest_2249 0.66 +1.3 2,398,895 - Psest_2249 0.66 +0.8 2,399,027 + +0.8 2,399,028 - +0.6 2,399,068 + +0.2 2,399,121 + -1.1
Or see this region's nucleotide sequence