Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1115

Experiment: Vancomycin 0.016 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1113 and Psest_1114 are separated by 42 nucleotidesPsest_1114 and Psest_1115 are separated by 21 nucleotidesPsest_1115 and Psest_1116 overlap by 8 nucleotidesPsest_1116 and Psest_1117 are separated by 84 nucleotides Psest_1113: Psest_1113 - peptide chain release factor 1, at 1,207,815 to 1,208,897 _1113 Psest_1114: Psest_1114 - protein-(glutamine-N5) methyltransferase, release factor-specific, at 1,208,940 to 1,209,767 _1114 Psest_1115: Psest_1115 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2, at 1,209,789 to 1,210,538 _1115 Psest_1116: Psest_1116 - glutamate racemase, at 1,210,531 to 1,211,325 _1116 Psest_1117: Psest_1117 - Lipid A 3-O-deacylase (PagL)., at 1,211,410 to 1,211,928 _1117 Position (kb) 1209 1210 1211Strain fitness (log2 ratio) -1 0 1 2 3at 1208.902 kb on + strandat 1208.902 kb on + strandat 1209.927 kb on + strand, within Psest_1115at 1209.927 kb on + strand, within Psest_1115at 1209.974 kb on + strand, within Psest_1115at 1210.340 kb on + strand, within Psest_1115at 1211.395 kb on + strandat 1211.395 kb on + strandat 1211.397 kb on + strandat 1211.397 kb on + strandat 1211.397 kb on + strandat 1211.398 kb on - strandat 1211.398 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vancomycin 0.016 mg/ml
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1,208,902 + +0.2
1,208,902 + -1.5
1,209,927 + Psest_1115 0.18 +1.7
1,209,927 + Psest_1115 0.18 -0.3
1,209,974 + Psest_1115 0.25 -0.9
1,210,340 + Psest_1115 0.73 +2.8
1,211,395 + +1.6
1,211,395 + -1.3
1,211,397 + -0.5
1,211,397 + -0.2
1,211,397 + -0.7
1,211,398 - -0.5
1,211,398 - +1.1

Or see this region's nucleotide sequence