Experiment: Vancomycin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1729 and Psest_1730 are separated by 40 nucleotides Psest_1730 and Psest_1731 are separated by 1 nucleotides Psest_1731 and Psest_1732 overlap by 1 nucleotides Psest_1732 and Psest_1733 are separated by 12 nucleotides
Psest_1729: Psest_1729 - flagellar motor switch protein FliM, at 1,835,251 to 1,836,222
_1729
Psest_1730: Psest_1730 - flagellar motor switch protein FliN, at 1,836,263 to 1,836,736
_1730
Psest_1731: Psest_1731 - flagellar biosynthetic protein FliO, at 1,836,738 to 1,837,157
_1731
Psest_1732: Psest_1732 - flagellar biosynthetic protein FliP, at 1,837,157 to 1,837,933
_1732
Psest_1733: Psest_1733 - flagellar biosynthetic protein FliQ, at 1,837,946 to 1,838,215
_1733
Position (kb)
1836
1837
1838 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1835.782 kb on + strand, within Psest_1729 at 1836.233 kb on + strand at 1836.234 kb on - strand at 1836.815 kb on + strand, within Psest_1731 at 1837.174 kb on - strand at 1837.269 kb on + strand, within Psest_1732 at 1837.276 kb on - strand, within Psest_1732 at 1837.280 kb on - strand, within Psest_1732 at 1837.280 kb on - strand, within Psest_1732 at 1837.539 kb on - strand, within Psest_1732 at 1837.539 kb on - strand, within Psest_1732 at 1837.661 kb on - strand, within Psest_1732 at 1837.661 kb on - strand, within Psest_1732 at 1837.661 kb on - strand, within Psest_1732 at 1837.931 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.016 mg/ml remove 1,835,782 + Psest_1729 0.55 +2.1 1,836,233 + -0.7 1,836,234 - +1.5 1,836,815 + Psest_1731 0.18 +1.6 1,837,174 - +2.2 1,837,269 + Psest_1732 0.14 +1.5 1,837,276 - Psest_1732 0.15 +2.0 1,837,280 - Psest_1732 0.16 -0.9 1,837,280 - Psest_1732 0.16 +2.8 1,837,539 - Psest_1732 0.49 -0.2 1,837,539 - Psest_1732 0.49 -0.6 1,837,661 - Psest_1732 0.65 +1.4 1,837,661 - Psest_1732 0.65 +2.5 1,837,661 - Psest_1732 0.65 -0.4 1,837,931 + +0.3
Or see this region's nucleotide sequence