Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3047 and Psest_3048 are separated by 63 nucleotides Psest_3048 and Psest_3049 overlap by 11 nucleotides Psest_3049 and Psest_3050 are separated by 80 nucleotides Psest_3050 and Psest_3051 are separated by 95 nucleotides
Psest_3047: Psest_3047 - methionyl-tRNA synthetase/methionyl-tRNA synthetase C-terminal region/beta chain, at 3,229,308 to 3,231,350
_3047
Psest_3048: Psest_3048 - hypothetical protein, at 3,231,414 to 3,231,626
_3048
Psest_3049: Psest_3049 - pseudouridine synthase, at 3,231,616 to 3,232,308
_3049
Psest_3050: Psest_3050 - tRNA_Gly_CCC, at 3,232,389 to 3,232,462
_3050
Psest_3051: Psest_3051 - Predicted Fe-S-cluster oxidoreductase, at 3,232,558 to 3,233,229
_3051
Position (kb)
3231
3232 Strain fitness (log2 ratio)
-1
0
1
2 at 3231.001 kb on + strand, within Psest_3047 at 3231.063 kb on - strand, within Psest_3047 at 3231.348 kb on + strand at 3231.349 kb on - strand at 3231.427 kb on + strand at 3231.427 kb on + strand at 3231.428 kb on - strand at 3231.428 kb on - strand at 3231.521 kb on + strand, within Psest_3048 at 3231.521 kb on + strand, within Psest_3048 at 3231.522 kb on - strand, within Psest_3048 at 3231.813 kb on + strand, within Psest_3049 at 3231.814 kb on - strand, within Psest_3049 at 3231.940 kb on - strand, within Psest_3049 at 3232.009 kb on + strand, within Psest_3049 at 3232.009 kb on + strand, within Psest_3049 at 3232.010 kb on - strand, within Psest_3049 at 3232.010 kb on - strand, within Psest_3049 at 3232.010 kb on - strand, within Psest_3049 at 3232.010 kb on - strand, within Psest_3049 at 3232.072 kb on + strand, within Psest_3049 at 3232.072 kb on + strand, within Psest_3049 at 3232.072 kb on + strand, within Psest_3049 at 3232.072 kb on + strand, within Psest_3049 at 3232.072 kb on + strand, within Psest_3049 at 3232.072 kb on + strand, within Psest_3049 at 3232.073 kb on - strand, within Psest_3049 at 3232.073 kb on - strand, within Psest_3049 at 3232.073 kb on - strand, within Psest_3049 at 3232.073 kb on - strand, within Psest_3049 at 3232.073 kb on - strand, within Psest_3049 at 3232.073 kb on - strand, within Psest_3049 at 3232.163 kb on - strand, within Psest_3049 at 3232.399 kb on + strand, within Psest_3050 at 3232.399 kb on + strand, within Psest_3050 at 3232.399 kb on + strand, within Psest_3050 at 3232.400 kb on - strand, within Psest_3050 at 3232.400 kb on - strand, within Psest_3050 at 3232.402 kb on - strand, within Psest_3050 at 3232.411 kb on - strand, within Psest_3050 at 3232.560 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,231,001 + Psest_3047 0.83 +1.5 3,231,063 - Psest_3047 0.86 +2.7 3,231,348 + -0.2 3,231,349 - +1.7 3,231,427 + +1.4 3,231,427 + -0.5 3,231,428 - -0.6 3,231,428 - +1.5 3,231,521 + Psest_3048 0.50 -1.4 3,231,521 + Psest_3048 0.50 -0.5 3,231,522 - Psest_3048 0.51 -0.6 3,231,813 + Psest_3049 0.28 -0.5 3,231,814 - Psest_3049 0.29 -1.7 3,231,940 - Psest_3049 0.47 +0.5 3,232,009 + Psest_3049 0.57 -0.6 3,232,009 + Psest_3049 0.57 -1.0 3,232,010 - Psest_3049 0.57 -0.6 3,232,010 - Psest_3049 0.57 -1.1 3,232,010 - Psest_3049 0.57 -0.1 3,232,010 - Psest_3049 0.57 +0.5 3,232,072 + Psest_3049 0.66 -0.3 3,232,072 + Psest_3049 0.66 -0.3 3,232,072 + Psest_3049 0.66 -0.0 3,232,072 + Psest_3049 0.66 -0.0 3,232,072 + Psest_3049 0.66 +1.9 3,232,072 + Psest_3049 0.66 -0.6 3,232,073 - Psest_3049 0.66 -0.6 3,232,073 - Psest_3049 0.66 +0.1 3,232,073 - Psest_3049 0.66 +2.1 3,232,073 - Psest_3049 0.66 -0.3 3,232,073 - Psest_3049 0.66 -1.0 3,232,073 - Psest_3049 0.66 +0.2 3,232,163 - Psest_3049 0.79 +0.9 3,232,399 + Psest_3050 0.14 -1.3 3,232,399 + Psest_3050 0.14 -0.1 3,232,399 + Psest_3050 0.14 -0.1 3,232,400 - Psest_3050 0.15 +1.2 3,232,400 - Psest_3050 0.15 -0.0 3,232,402 - Psest_3050 0.18 +0.3 3,232,411 - Psest_3050 0.30 -1.0 3,232,560 - -0.2
Or see this region's nucleotide sequence