Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2303 and Psest_2304 are separated by 140 nucleotides Psest_2304 and Psest_2305 are separated by 6 nucleotides Psest_2305 and Psest_2306 overlap by 4 nucleotides
Psest_2303: Psest_2303 - glutaminyl-tRNA synthetase, at 2,453,444 to 2,455,174
_2303
Psest_2304: Psest_2304 - Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family, at 2,455,315 to 2,455,809
_2304
Psest_2305: Psest_2305 - UDP-2,3-diacylglucosamine hydrolase, at 2,455,816 to 2,456,538
_2305
Psest_2306: Psest_2306 - Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA, at 2,456,535 to 2,457,185
_2306
Position (kb)
2455
2456 Strain fitness (log2 ratio)
-1
0
1 at 2455.402 kb on + strand, within Psest_2304 at 2456.536 kb on + strand at 2456.537 kb on - strand at 2456.659 kb on + strand, within Psest_2306 at 2456.674 kb on + strand, within Psest_2306 at 2456.674 kb on + strand, within Psest_2306 at 2456.674 kb on + strand, within Psest_2306 at 2456.675 kb on - strand, within Psest_2306 at 2456.717 kb on - strand, within Psest_2306 at 2456.727 kb on + strand, within Psest_2306 at 2456.728 kb on - strand, within Psest_2306 at 2456.728 kb on - strand, within Psest_2306
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,455,402 + Psest_2304 0.18 -1.4 2,456,536 + +0.4 2,456,537 - -0.0 2,456,659 + Psest_2306 0.19 -1.6 2,456,674 + Psest_2306 0.21 +1.1 2,456,674 + Psest_2306 0.21 -1.0 2,456,674 + Psest_2306 0.21 +0.2 2,456,675 - Psest_2306 0.22 -1.5 2,456,717 - Psest_2306 0.28 -1.0 2,456,727 + Psest_2306 0.29 +0.5 2,456,728 - Psest_2306 0.30 -0.7 2,456,728 - Psest_2306 0.30 +0.8
Or see this region's nucleotide sequence