Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0502 and Psest_0503 are separated by 77 nucleotides Psest_0503 and Psest_0504 are separated by 225 nucleotides Psest_0504 and Psest_0505 overlap by 4 nucleotides Psest_0505 and Psest_0506 are separated by 10 nucleotides
Psest_0502: Psest_0502 - Uncharacterized protein conserved in bacteria, at 556,307 to 557,980
_0502
Psest_0503: Psest_0503 - Acyl dehydratase, at 558,058 to 558,513
_0503
Psest_0504: Psest_0504 - Putative effector of murein hydrolase LrgA, at 558,739 to 559,101
_0504
Psest_0505: Psest_0505 - Putative effector of murein hydrolase, at 559,098 to 559,817
_0505
Psest_0506: Psest_0506 - Uncharacterized protein, similar to the N-terminal domain of Lon protease, at 559,828 to 560,412
_0506
Position (kb) 558
559
560 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 557.809 kb on - strand, within Psest_0502 at 557.809 kb on - strand, within Psest_0502 at 557.811 kb on + strand, within Psest_0502 at 557.811 kb on + strand, within Psest_0502 at 557.811 kb on + strand, within Psest_0502 at 557.811 kb on + strand, within Psest_0502 at 557.811 kb on + strand, within Psest_0502 at 557.811 kb on + strand, within Psest_0502 at 557.811 kb on + strand, within Psest_0502 at 557.811 kb on + strand, within Psest_0502 at 557.811 kb on + strand, within Psest_0502 at 557.812 kb on - strand, within Psest_0502 at 557.812 kb on - strand, within Psest_0502 at 557.812 kb on - strand, within Psest_0502 at 557.812 kb on - strand, within Psest_0502 at 557.812 kb on - strand, within Psest_0502 at 557.812 kb on - strand, within Psest_0502 at 558.012 kb on + strand at 558.013 kb on - strand at 558.092 kb on + strand at 558.092 kb on + strand at 558.092 kb on + strand at 558.093 kb on - strand at 558.196 kb on + strand, within Psest_0503 at 558.268 kb on - strand, within Psest_0503 at 558.480 kb on - strand at 558.480 kb on - strand at 558.480 kb on - strand at 558.480 kb on - strand at 558.535 kb on + strand at 558.535 kb on + strand at 558.536 kb on - strand at 558.536 kb on - strand at 558.536 kb on - strand at 558.562 kb on - strand at 558.617 kb on - strand at 558.617 kb on - strand at 558.617 kb on - strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.712 kb on - strand at 558.712 kb on - strand at 558.712 kb on - strand at 558.712 kb on - strand at 558.712 kb on - strand at 558.712 kb on - strand at 558.712 kb on - strand at 558.920 kb on - strand, within Psest_0504 at 558.964 kb on + strand, within Psest_0504 at 558.964 kb on + strand, within Psest_0504 at 558.965 kb on - strand, within Psest_0504 at 558.965 kb on - strand, within Psest_0504 at 558.966 kb on + strand, within Psest_0504 at 558.966 kb on + strand, within Psest_0504 at 558.966 kb on + strand, within Psest_0504 at 558.967 kb on - strand, within Psest_0504 at 559.116 kb on + strand at 559.117 kb on - strand at 559.166 kb on + strand at 559.167 kb on - strand at 559.167 kb on - strand at 559.191 kb on + strand, within Psest_0505 at 559.192 kb on - strand, within Psest_0505 at 559.192 kb on - strand, within Psest_0505 at 559.219 kb on + strand, within Psest_0505 at 559.287 kb on + strand, within Psest_0505 at 559.288 kb on - strand, within Psest_0505 at 559.288 kb on - strand, within Psest_0505 at 559.296 kb on + strand, within Psest_0505 at 559.859 kb on + strand at 559.860 kb on - strand at 559.860 kb on - strand at 559.860 kb on - strand at 559.860 kb on - strand at 559.906 kb on + strand, within Psest_0506 at 559.907 kb on - strand, within Psest_0506 at 559.955 kb on + strand, within Psest_0506 at 559.955 kb on + strand, within Psest_0506 at 559.956 kb on - strand, within Psest_0506
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 557,809 - Psest_0502 0.90 +0.1 557,809 - Psest_0502 0.90 -0.5 557,811 + Psest_0502 0.90 -0.4 557,811 + Psest_0502 0.90 +0.2 557,811 + Psest_0502 0.90 +0.2 557,811 + Psest_0502 0.90 +0.3 557,811 + Psest_0502 0.90 -0.8 557,811 + Psest_0502 0.90 -0.3 557,811 + Psest_0502 0.90 +0.0 557,811 + Psest_0502 0.90 -3.1 557,811 + Psest_0502 0.90 -0.2 557,812 - Psest_0502 0.90 +0.8 557,812 - Psest_0502 0.90 -0.7 557,812 - Psest_0502 0.90 -3.0 557,812 - Psest_0502 0.90 +2.0 557,812 - Psest_0502 0.90 +0.1 557,812 - Psest_0502 0.90 +2.6 558,012 + -0.4 558,013 - -0.5 558,092 + -0.5 558,092 + -0.2 558,092 + -0.7 558,093 - -0.6 558,196 + Psest_0503 0.30 +0.1 558,268 - Psest_0503 0.46 +0.2 558,480 - -0.3 558,480 - -0.2 558,480 - -2.4 558,480 - +1.3 558,535 + -0.3 558,535 + +1.2 558,536 - +0.0 558,536 - -0.7 558,536 - -1.8 558,562 - -1.7 558,617 - -0.7 558,617 - -0.4 558,617 - -1.7 558,711 + -1.7 558,711 + -0.3 558,711 + -0.5 558,711 + +0.8 558,711 + -0.1 558,711 + -0.7 558,711 + -1.8 558,711 + -0.6 558,711 + +0.3 558,711 + +0.4 558,712 - -0.3 558,712 - -0.3 558,712 - +0.3 558,712 - +1.5 558,712 - +1.1 558,712 - +0.3 558,712 - -0.2 558,920 - Psest_0504 0.50 -0.1 558,964 + Psest_0504 0.62 -0.8 558,964 + Psest_0504 0.62 -1.2 558,965 - Psest_0504 0.62 -1.5 558,965 - Psest_0504 0.62 -0.7 558,966 + Psest_0504 0.63 +0.1 558,966 + Psest_0504 0.63 -0.8 558,966 + Psest_0504 0.63 -0.8 558,967 - Psest_0504 0.63 -0.8 559,116 + +0.3 559,117 - +0.2 559,166 + -0.0 559,167 - -2.0 559,167 - -0.4 559,191 + Psest_0505 0.13 +1.2 559,192 - Psest_0505 0.13 +0.3 559,192 - Psest_0505 0.13 -0.3 559,219 + Psest_0505 0.17 +0.9 559,287 + Psest_0505 0.26 -0.6 559,288 - Psest_0505 0.26 -0.8 559,288 - Psest_0505 0.26 +1.0 559,296 + Psest_0505 0.28 +0.4 559,859 + -1.2 559,860 - -0.9 559,860 - +0.3 559,860 - +0.3 559,860 - +4.7 559,906 + Psest_0506 0.13 +0.1 559,907 - Psest_0506 0.14 +0.9 559,955 + Psest_0506 0.22 +0.6 559,955 + Psest_0506 0.22 +0.4 559,956 - Psest_0506 0.22 +1.0
Or see this region's nucleotide sequence