Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1944 and Psest_1945 are separated by 138 nucleotides Psest_1945 and Psest_1946 are separated by 158 nucleotides Psest_1946 and Psest_1947 are separated by 111 nucleotides
Psest_1944: Psest_1944 - NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form (from data) , at 2,057,299 to 2,062,149
_1944
Psest_1945: Psest_1945 - hypothetical protein, at 2,062,288 to 2,063,202
_1945
Psest_1946: Psest_1946 - Protein of unknown function (DUF2835)., at 2,063,361 to 2,063,582
_1946
Psest_1947: Psest_1947 - dihydroorotate dehydrogenase, subfamily 2, at 2,063,694 to 2,064,725
_1947
Position (kb)
2062
2063
2064 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2061.683 kb on - strand at 2061.743 kb on - strand at 2061.743 kb on - strand at 2061.811 kb on + strand at 2061.812 kb on - strand at 2061.847 kb on + strand at 2061.847 kb on + strand at 2061.847 kb on + strand at 2061.848 kb on - strand at 2062.084 kb on + strand at 2062.084 kb on + strand at 2062.084 kb on + strand at 2062.085 kb on - strand at 2062.091 kb on + strand at 2062.093 kb on + strand at 2062.093 kb on + strand at 2062.093 kb on + strand at 2062.093 kb on + strand at 2062.093 kb on + strand at 2062.093 kb on + strand at 2062.093 kb on + strand at 2062.093 kb on + strand at 2062.093 kb on + strand at 2062.093 kb on + strand at 2062.093 kb on + strand at 2062.094 kb on - strand at 2062.094 kb on - strand at 2062.245 kb on + strand at 2062.260 kb on - strand at 2062.447 kb on - strand, within Psest_1945 at 2062.453 kb on + strand, within Psest_1945 at 2062.454 kb on - strand, within Psest_1945 at 2062.644 kb on - strand, within Psest_1945 at 2062.692 kb on - strand, within Psest_1945 at 2062.692 kb on - strand, within Psest_1945 at 2062.713 kb on + strand, within Psest_1945 at 2062.790 kb on + strand, within Psest_1945 at 2062.790 kb on + strand, within Psest_1945 at 2062.790 kb on + strand, within Psest_1945 at 2062.791 kb on - strand, within Psest_1945 at 2063.341 kb on + strand at 2063.341 kb on + strand at 2063.341 kb on + strand at 2063.367 kb on + strand at 2063.368 kb on - strand at 2063.368 kb on - strand at 2063.368 kb on - strand at 2063.413 kb on + strand, within Psest_1946 at 2063.413 kb on + strand, within Psest_1946 at 2063.413 kb on + strand, within Psest_1946 at 2063.414 kb on - strand, within Psest_1946 at 2063.414 kb on - strand, within Psest_1946 at 2063.416 kb on - strand, within Psest_1946 at 2063.416 kb on - strand, within Psest_1946 at 2063.517 kb on + strand, within Psest_1946 at 2063.517 kb on + strand, within Psest_1946 at 2063.517 kb on + strand, within Psest_1946 at 2063.518 kb on - strand, within Psest_1946 at 2063.518 kb on - strand, within Psest_1946 at 2063.580 kb on + strand at 2063.580 kb on + strand at 2063.693 kb on + strand at 2063.693 kb on + strand at 2063.693 kb on + strand at 2063.693 kb on + strand at 2063.693 kb on + strand at 2063.693 kb on + strand at 2063.693 kb on + strand at 2063.693 kb on + strand at 2063.693 kb on + strand at 2063.693 kb on + strand at 2063.697 kb on + strand at 2063.697 kb on + strand at 2063.697 kb on + strand at 2063.697 kb on + strand at 2063.728 kb on + strand at 2063.864 kb on - strand, within Psest_1947 at 2064.049 kb on + strand, within Psest_1947 at 2064.177 kb on + strand, within Psest_1947 at 2064.192 kb on + strand, within Psest_1947 at 2064.192 kb on + strand, within Psest_1947 at 2064.192 kb on + strand, within Psest_1947 at 2064.192 kb on + strand, within Psest_1947
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 2,061,683 - +1.3 2,061,743 - -0.1 2,061,743 - +0.8 2,061,811 + +0.4 2,061,812 - -0.8 2,061,847 + +0.6 2,061,847 + +0.7 2,061,847 + +0.7 2,061,848 - -0.9 2,062,084 + +0.7 2,062,084 + +0.8 2,062,084 + +0.7 2,062,085 - -0.1 2,062,091 + -0.3 2,062,093 + -0.4 2,062,093 + +0.5 2,062,093 + +2.0 2,062,093 + -0.6 2,062,093 + +1.7 2,062,093 + -1.2 2,062,093 + +1.5 2,062,093 + +0.7 2,062,093 + -1.1 2,062,093 + -1.0 2,062,093 + -0.9 2,062,094 - -0.4 2,062,094 - +1.1 2,062,245 + -0.6 2,062,260 - +0.8 2,062,447 - Psest_1945 0.17 -0.6 2,062,453 + Psest_1945 0.18 -2.0 2,062,454 - Psest_1945 0.18 +1.9 2,062,644 - Psest_1945 0.39 +1.0 2,062,692 - Psest_1945 0.44 -0.1 2,062,692 - Psest_1945 0.44 +1.6 2,062,713 + Psest_1945 0.46 +0.7 2,062,790 + Psest_1945 0.55 -0.1 2,062,790 + Psest_1945 0.55 +2.8 2,062,790 + Psest_1945 0.55 -0.5 2,062,791 - Psest_1945 0.55 +0.3 2,063,341 + +1.1 2,063,341 + -3.3 2,063,341 + -1.1 2,063,367 + +0.2 2,063,368 - -1.1 2,063,368 - +0.1 2,063,368 - +1.5 2,063,413 + Psest_1946 0.23 +0.6 2,063,413 + Psest_1946 0.23 -0.8 2,063,413 + Psest_1946 0.23 -1.6 2,063,414 - Psest_1946 0.24 +0.2 2,063,414 - Psest_1946 0.24 +1.3 2,063,416 - Psest_1946 0.25 +0.1 2,063,416 - Psest_1946 0.25 -1.2 2,063,517 + Psest_1946 0.70 +0.4 2,063,517 + Psest_1946 0.70 +1.0 2,063,517 + Psest_1946 0.70 -1.2 2,063,518 - Psest_1946 0.71 -0.8 2,063,518 - Psest_1946 0.71 +0.0 2,063,580 + -0.2 2,063,580 + -0.0 2,063,693 + -1.5 2,063,693 + -2.1 2,063,693 + +0.1 2,063,693 + -0.5 2,063,693 + +0.6 2,063,693 + -0.9 2,063,693 + +0.5 2,063,693 + +1.2 2,063,693 + +1.1 2,063,693 + -1.7 2,063,697 + -1.2 2,063,697 + +0.1 2,063,697 + -0.5 2,063,697 + +0.1 2,063,728 + +1.1 2,063,864 - Psest_1947 0.16 +1.1 2,064,049 + Psest_1947 0.34 +1.1 2,064,177 + Psest_1947 0.47 +0.1 2,064,192 + Psest_1947 0.48 +0.1 2,064,192 + Psest_1947 0.48 +1.1 2,064,192 + Psest_1947 0.48 +1.1 2,064,192 + Psest_1947 0.48 +1.1
Or see this region's nucleotide sequence