Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1858 and Psest_1859 are separated by 13 nucleotides Psest_1859 and Psest_1860 are separated by 87 nucleotides Psest_1860 and Psest_1861 are separated by 34 nucleotides
Psest_1858: Psest_1858 - hypothetical protein, at 1,963,425 to 1,963,550
_1858
Psest_1859: Psest_1859 - Glutathione S-transferase, at 1,963,564 to 1,964,235
_1859
Psest_1860: Psest_1860 - Uncharacterized conserved protein, at 1,964,323 to 1,964,910
_1860
Psest_1861: Psest_1861 - type I secretion membrane fusion protein, HlyD family, at 1,964,945 to 1,966,297
_1861
Position (kb)
1964
1965 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1963.362 kb on + strand at 1963.362 kb on + strand at 1963.363 kb on - strand at 1963.363 kb on - strand at 1963.363 kb on - strand at 1963.395 kb on + strand at 1963.396 kb on - strand at 1963.403 kb on + strand at 1963.608 kb on + strand at 1963.610 kb on + strand at 1963.610 kb on + strand at 1963.611 kb on - strand at 1963.627 kb on + strand at 1963.627 kb on + strand at 1963.628 kb on - strand at 1963.628 kb on - strand at 1963.628 kb on - strand at 1963.629 kb on + strand at 1963.714 kb on + strand, within Psest_1859 at 1963.714 kb on + strand, within Psest_1859 at 1963.714 kb on + strand, within Psest_1859 at 1963.714 kb on + strand, within Psest_1859 at 1963.715 kb on - strand, within Psest_1859 at 1963.715 kb on - strand, within Psest_1859 at 1963.734 kb on + strand, within Psest_1859 at 1963.734 kb on + strand, within Psest_1859 at 1963.778 kb on + strand, within Psest_1859 at 1963.779 kb on - strand, within Psest_1859 at 1963.779 kb on - strand, within Psest_1859 at 1963.779 kb on - strand, within Psest_1859 at 1963.795 kb on + strand, within Psest_1859 at 1963.795 kb on + strand, within Psest_1859 at 1963.937 kb on + strand, within Psest_1859 at 1964.159 kb on + strand, within Psest_1859 at 1964.159 kb on + strand, within Psest_1859 at 1964.160 kb on - strand, within Psest_1859 at 1964.160 kb on - strand, within Psest_1859 at 1964.160 kb on - strand, within Psest_1859 at 1964.250 kb on - strand at 1964.268 kb on + strand at 1964.545 kb on + strand, within Psest_1860 at 1964.545 kb on + strand, within Psest_1860 at 1964.546 kb on - strand, within Psest_1860 at 1964.664 kb on + strand, within Psest_1860 at 1964.881 kb on + strand at 1964.946 kb on + strand at 1964.947 kb on - strand at 1964.997 kb on + strand at 1964.997 kb on + strand at 1964.997 kb on + strand at 1964.997 kb on + strand at 1964.998 kb on - strand at 1964.998 kb on - strand at 1964.998 kb on - strand at 1964.998 kb on - strand at 1965.118 kb on - strand, within Psest_1861 at 1965.124 kb on + strand, within Psest_1861 at 1965.124 kb on + strand, within Psest_1861 at 1965.124 kb on + strand, within Psest_1861 at 1965.125 kb on - strand, within Psest_1861 at 1965.198 kb on + strand, within Psest_1861 at 1965.198 kb on + strand, within Psest_1861 at 1965.198 kb on + strand, within Psest_1861 at 1965.199 kb on - strand, within Psest_1861 at 1965.267 kb on - strand, within Psest_1861 at 1965.333 kb on - strand, within Psest_1861 at 1965.333 kb on - strand, within Psest_1861 at 1965.381 kb on - strand, within Psest_1861 at 1965.457 kb on + strand, within Psest_1861 at 1965.457 kb on + strand, within Psest_1861 at 1965.457 kb on + strand, within Psest_1861 at 1965.457 kb on + strand, within Psest_1861 at 1965.458 kb on - strand, within Psest_1861 at 1965.458 kb on - strand, within Psest_1861 at 1965.458 kb on - strand, within Psest_1861 at 1965.458 kb on - strand, within Psest_1861 at 1965.600 kb on - strand, within Psest_1861 at 1965.651 kb on - strand, within Psest_1861 at 1965.686 kb on - strand, within Psest_1861 at 1965.706 kb on - strand, within Psest_1861 at 1965.706 kb on - strand, within Psest_1861 at 1965.811 kb on - strand, within Psest_1861 at 1965.811 kb on - strand, within Psest_1861
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 1,963,362 + +0.4 1,963,362 + -0.5 1,963,363 - +0.7 1,963,363 - -1.0 1,963,363 - +2.7 1,963,395 + -0.5 1,963,396 - -0.3 1,963,403 + -1.9 1,963,608 + +0.2 1,963,610 + +0.5 1,963,610 + +1.5 1,963,611 - -1.1 1,963,627 + +0.1 1,963,627 + +0.6 1,963,628 - +0.2 1,963,628 - +0.3 1,963,628 - +0.8 1,963,629 + -0.3 1,963,714 + Psest_1859 0.22 -1.7 1,963,714 + Psest_1859 0.22 +0.7 1,963,714 + Psest_1859 0.22 -0.8 1,963,714 + Psest_1859 0.22 -0.5 1,963,715 - Psest_1859 0.22 -0.2 1,963,715 - Psest_1859 0.22 +0.4 1,963,734 + Psest_1859 0.25 +0.2 1,963,734 + Psest_1859 0.25 -0.4 1,963,778 + Psest_1859 0.32 -0.5 1,963,779 - Psest_1859 0.32 +0.2 1,963,779 - Psest_1859 0.32 -0.3 1,963,779 - Psest_1859 0.32 +1.0 1,963,795 + Psest_1859 0.34 +1.2 1,963,795 + Psest_1859 0.34 +0.6 1,963,937 + Psest_1859 0.56 +0.3 1,964,159 + Psest_1859 0.89 -1.2 1,964,159 + Psest_1859 0.89 -2.4 1,964,160 - Psest_1859 0.89 +0.1 1,964,160 - Psest_1859 0.89 +0.8 1,964,160 - Psest_1859 0.89 +0.2 1,964,250 - -0.7 1,964,268 + +0.5 1,964,545 + Psest_1860 0.38 -0.9 1,964,545 + Psest_1860 0.38 +2.3 1,964,546 - Psest_1860 0.38 +0.6 1,964,664 + Psest_1860 0.58 +0.4 1,964,881 + +0.4 1,964,946 + +1.3 1,964,947 - +1.9 1,964,997 + -0.8 1,964,997 + +0.7 1,964,997 + +0.4 1,964,997 + -1.1 1,964,998 - -0.6 1,964,998 - +1.4 1,964,998 - -0.5 1,964,998 - -0.5 1,965,118 - Psest_1861 0.13 -0.8 1,965,124 + Psest_1861 0.13 +0.4 1,965,124 + Psest_1861 0.13 -0.3 1,965,124 + Psest_1861 0.13 +0.3 1,965,125 - Psest_1861 0.13 +0.7 1,965,198 + Psest_1861 0.19 -0.4 1,965,198 + Psest_1861 0.19 +1.2 1,965,198 + Psest_1861 0.19 -0.1 1,965,199 - Psest_1861 0.19 -1.1 1,965,267 - Psest_1861 0.24 -0.8 1,965,333 - Psest_1861 0.29 +0.4 1,965,333 - Psest_1861 0.29 -0.5 1,965,381 - Psest_1861 0.32 -0.4 1,965,457 + Psest_1861 0.38 -0.1 1,965,457 + Psest_1861 0.38 +2.1 1,965,457 + Psest_1861 0.38 -0.3 1,965,457 + Psest_1861 0.38 +0.3 1,965,458 - Psest_1861 0.38 -0.0 1,965,458 - Psest_1861 0.38 +0.9 1,965,458 - Psest_1861 0.38 -0.8 1,965,458 - Psest_1861 0.38 -0.3 1,965,600 - Psest_1861 0.48 -1.8 1,965,651 - Psest_1861 0.52 +0.2 1,965,686 - Psest_1861 0.55 -0.9 1,965,706 - Psest_1861 0.56 +2.6 1,965,706 - Psest_1861 0.56 +0.5 1,965,811 - Psest_1861 0.64 +0.1 1,965,811 - Psest_1861 0.64 +0.9
Or see this region's nucleotide sequence