Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1661 and Psest_1662 overlap by 4 nucleotides Psest_1662 and Psest_1663 are separated by 146 nucleotides Psest_1663 and Psest_1664 are separated by 146 nucleotides Psest_1664 and Psest_1665 overlap by 4 nucleotides
Psest_1661: Psest_1661 - Membrane-associated lipoprotein involved in thiamine biosynthesis, at 1,770,731 to 1,771,783
_1661
Psest_1662: Psest_1662 - Uncharacterized protein conserved in bacteria, at 1,771,780 to 1,772,007
_1662
Psest_1663: Psest_1663 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes, at 1,772,154 to 1,773,548
_1663
Psest_1664: Psest_1664 - Glycerophosphoryl diester phosphodiesterase, at 1,773,695 to 1,774,417
_1664
Psest_1665: Psest_1665 - hypothetical protein, at 1,774,414 to 1,774,722
_1665
Position (kb)
1772
1773
1774 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1771.647 kb on + strand, within Psest_1661 at 1772.127 kb on + strand at 1772.151 kb on + strand at 1772.151 kb on + strand at 1772.151 kb on + strand at 1772.151 kb on + strand at 1772.151 kb on + strand at 1772.152 kb on - strand at 1772.164 kb on - strand at 1772.164 kb on - strand at 1772.637 kb on - strand, within Psest_1663 at 1772.641 kb on - strand, within Psest_1663 at 1772.824 kb on - strand, within Psest_1663 at 1773.045 kb on + strand, within Psest_1663 at 1773.069 kb on + strand, within Psest_1663 at 1773.117 kb on + strand, within Psest_1663 at 1773.198 kb on + strand, within Psest_1663 at 1773.198 kb on + strand, within Psest_1663 at 1773.198 kb on + strand, within Psest_1663 at 1773.199 kb on - strand, within Psest_1663 at 1773.462 kb on + strand at 1773.637 kb on + strand at 1773.637 kb on + strand at 1773.878 kb on + strand, within Psest_1664 at 1773.879 kb on - strand, within Psest_1664 at 1773.879 kb on - strand, within Psest_1664 at 1773.927 kb on + strand, within Psest_1664 at 1773.928 kb on - strand, within Psest_1664 at 1773.928 kb on - strand, within Psest_1664 at 1773.928 kb on - strand, within Psest_1664 at 1773.928 kb on - strand, within Psest_1664 at 1773.950 kb on + strand, within Psest_1664 at 1773.950 kb on + strand, within Psest_1664 at 1773.951 kb on - strand, within Psest_1664 at 1774.133 kb on + strand, within Psest_1664 at 1774.134 kb on - strand, within Psest_1664 at 1774.197 kb on + strand, within Psest_1664 at 1774.198 kb on - strand, within Psest_1664 at 1774.198 kb on - strand, within Psest_1664 at 1774.198 kb on - strand, within Psest_1664 at 1774.328 kb on + strand, within Psest_1664 at 1774.329 kb on - strand, within Psest_1664 at 1774.329 kb on - strand, within Psest_1664 at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.405 kb on - strand at 1774.405 kb on - strand at 1774.405 kb on - strand at 1774.405 kb on - strand at 1774.405 kb on - strand at 1774.423 kb on + strand at 1774.424 kb on - strand at 1774.449 kb on + strand, within Psest_1665 at 1774.449 kb on + strand, within Psest_1665 at 1774.449 kb on + strand, within Psest_1665 at 1774.450 kb on - strand, within Psest_1665 at 1774.450 kb on - strand, within Psest_1665 at 1774.496 kb on + strand, within Psest_1665 at 1774.496 kb on + strand, within Psest_1665 at 1774.496 kb on + strand, within Psest_1665
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 1,771,647 + Psest_1661 0.87 +0.1 1,772,127 + -0.2 1,772,151 + -1.0 1,772,151 + -0.9 1,772,151 + +0.6 1,772,151 + -1.2 1,772,151 + -0.4 1,772,152 - -0.6 1,772,164 - -0.5 1,772,164 - +1.1 1,772,637 - Psest_1663 0.35 +1.9 1,772,641 - Psest_1663 0.35 +2.4 1,772,824 - Psest_1663 0.48 -0.9 1,773,045 + Psest_1663 0.64 -0.3 1,773,069 + Psest_1663 0.66 +3.8 1,773,117 + Psest_1663 0.69 +0.8 1,773,198 + Psest_1663 0.75 -0.2 1,773,198 + Psest_1663 0.75 +2.9 1,773,198 + Psest_1663 0.75 +1.1 1,773,199 - Psest_1663 0.75 -0.3 1,773,462 + -0.9 1,773,637 + -0.4 1,773,637 + +1.2 1,773,878 + Psest_1664 0.25 +0.2 1,773,879 - Psest_1664 0.25 -1.3 1,773,879 - Psest_1664 0.25 -0.1 1,773,927 + Psest_1664 0.32 -0.4 1,773,928 - Psest_1664 0.32 +1.2 1,773,928 - Psest_1664 0.32 -1.2 1,773,928 - Psest_1664 0.32 +1.2 1,773,928 - Psest_1664 0.32 +0.1 1,773,950 + Psest_1664 0.35 +0.7 1,773,950 + Psest_1664 0.35 -0.2 1,773,951 - Psest_1664 0.35 +1.3 1,774,133 + Psest_1664 0.61 -0.5 1,774,134 - Psest_1664 0.61 -1.2 1,774,197 + Psest_1664 0.69 -2.2 1,774,198 - Psest_1664 0.70 -0.9 1,774,198 - Psest_1664 0.70 +0.2 1,774,198 - Psest_1664 0.70 -1.3 1,774,328 + Psest_1664 0.88 -0.6 1,774,329 - Psest_1664 0.88 +1.3 1,774,329 - Psest_1664 0.88 -1.4 1,774,404 + +0.6 1,774,404 + -0.7 1,774,404 + -1.4 1,774,404 + +0.7 1,774,404 + +1.5 1,774,404 + +0.4 1,774,404 + -0.4 1,774,404 + -1.1 1,774,405 - +0.6 1,774,405 - -2.0 1,774,405 - -1.4 1,774,405 - +0.2 1,774,405 - +3.0 1,774,423 + +1.8 1,774,424 - +0.6 1,774,449 + Psest_1665 0.11 -0.2 1,774,449 + Psest_1665 0.11 +0.4 1,774,449 + Psest_1665 0.11 -0.2 1,774,450 - Psest_1665 0.12 -1.4 1,774,450 - Psest_1665 0.12 +0.2 1,774,496 + Psest_1665 0.27 -0.2 1,774,496 + Psest_1665 0.27 -0.0 1,774,496 + Psest_1665 0.27 -0.5
Or see this region's nucleotide sequence