Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0011 and Psest_0012 are separated by 107 nucleotides Psest_0012 and Psest_0013 are separated by 108 nucleotides Psest_0013 and Psest_0014 are separated by 26 nucleotides Psest_0014 and Psest_0015 are separated by 25 nucleotides Psest_0015 and Psest_0016 are separated by 146 nucleotides
Psest_0011: Psest_0011 - glycyl-tRNA synthetase, tetrameric type, alpha subunit, at 13,407 to 14,354
_0011
Psest_0012: Psest_0012 - DNA-3-methyladenine glycosylase I, at 14,462 to 15,019
_0012
Psest_0013: Psest_0013 - Lauroyl/myristoyl acyltransferase, at 15,128 to 16,015
_0013
Psest_0014: Psest_0014 - PilZ domain., at 16,042 to 16,308
_0014
Psest_0015: Psest_0015 - Tetratricopeptide repeat., at 16,334 to 16,651
_0015
Psest_0016: Psest_0016 - ectoine/hydroxyectoine ABC transporter, ATP-binding protein, at 16,798 to 17,634
_0016
Position (kb) 15
16
17 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 14.395 kb on - strand at 14.395 kb on - strand at 14.395 kb on - strand at 14.498 kb on + strand at 14.498 kb on + strand at 14.498 kb on + strand at 14.498 kb on + strand at 14.498 kb on + strand at 14.499 kb on - strand at 14.499 kb on - strand at 14.936 kb on + strand, within Psest_0012 at 14.936 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.942 kb on + strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.943 kb on - strand, within Psest_0012 at 14.994 kb on - strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.033 kb on + strand at 15.034 kb on - strand at 15.094 kb on + strand at 15.094 kb on + strand at 15.094 kb on + strand at 15.095 kb on - strand at 15.518 kb on + strand, within Psest_0013 at 15.518 kb on + strand, within Psest_0013 at 15.519 kb on - strand, within Psest_0013 at 15.548 kb on + strand, within Psest_0013 at 15.548 kb on + strand, within Psest_0013 at 15.549 kb on - strand, within Psest_0013 at 15.549 kb on - strand, within Psest_0013 at 15.723 kb on + strand, within Psest_0013 at 15.724 kb on - strand, within Psest_0013 at 15.724 kb on - strand, within Psest_0013 at 15.724 kb on - strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.932 kb on + strand at 15.932 kb on + strand at 15.932 kb on + strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.944 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 16.041 kb on + strand at 16.042 kb on - strand at 16.042 kb on - strand at 16.156 kb on + strand, within Psest_0014 at 16.156 kb on + strand, within Psest_0014 at 16.156 kb on + strand, within Psest_0014 at 16.156 kb on + strand, within Psest_0014 at 16.157 kb on - strand, within Psest_0014 at 16.181 kb on + strand, within Psest_0014 at 16.181 kb on + strand, within Psest_0014 at 16.182 kb on - strand, within Psest_0014 at 16.506 kb on + strand, within Psest_0015 at 16.575 kb on + strand, within Psest_0015 at 16.575 kb on + strand, within Psest_0015 at 16.575 kb on + strand, within Psest_0015 at 16.576 kb on - strand, within Psest_0015 at 16.698 kb on + strand at 16.699 kb on - strand at 16.733 kb on + strand at 16.734 kb on - strand at 16.734 kb on - strand at 16.734 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 14,395 - -0.2 14,395 - +1.3 14,395 - +0.0 14,498 + -1.0 14,498 + +1.3 14,498 + -0.3 14,498 + +1.0 14,498 + +1.2 14,499 - +0.8 14,499 - -0.1 14,936 + Psest_0012 0.85 +0.7 14,936 + Psest_0012 0.85 -0.2 14,942 + Psest_0012 0.86 +3.0 14,942 + Psest_0012 0.86 +1.1 14,942 + Psest_0012 0.86 -0.3 14,942 + Psest_0012 0.86 -1.5 14,942 + Psest_0012 0.86 -0.1 14,942 + Psest_0012 0.86 +0.3 14,942 + Psest_0012 0.86 +0.8 14,942 + Psest_0012 0.86 +0.6 14,942 + Psest_0012 0.86 +0.9 14,942 + Psest_0012 0.86 -0.5 14,942 + Psest_0012 0.86 -1.9 14,942 + Psest_0012 0.86 -0.8 14,943 - Psest_0012 0.86 +0.3 14,943 - Psest_0012 0.86 -1.0 14,943 - Psest_0012 0.86 -0.1 14,943 - Psest_0012 0.86 +2.1 14,943 - Psest_0012 0.86 +0.3 14,943 - Psest_0012 0.86 +1.0 14,994 - -0.2 15,033 + +1.3 15,033 + +1.6 15,033 + +0.1 15,034 - -1.8 15,094 + +1.3 15,094 + -0.5 15,094 + -0.7 15,095 - +0.0 15,518 + Psest_0013 0.44 -0.9 15,518 + Psest_0013 0.44 +0.1 15,519 - Psest_0013 0.44 -1.8 15,548 + Psest_0013 0.47 -0.5 15,548 + Psest_0013 0.47 +1.3 15,549 - Psest_0013 0.47 +1.0 15,549 - Psest_0013 0.47 +0.8 15,723 + Psest_0013 0.67 +1.8 15,724 - Psest_0013 0.67 +0.3 15,724 - Psest_0013 0.67 +2.2 15,724 - Psest_0013 0.67 +1.3 15,878 + Psest_0013 0.84 -1.6 15,878 + Psest_0013 0.84 -0.8 15,878 + Psest_0013 0.84 -0.0 15,878 + Psest_0013 0.84 +0.8 15,878 + Psest_0013 0.84 -1.2 15,878 + Psest_0013 0.84 +2.4 15,878 + Psest_0013 0.84 +2.0 15,878 + Psest_0013 0.84 +0.3 15,878 + Psest_0013 0.84 +0.2 15,878 + Psest_0013 0.84 +0.4 15,878 + Psest_0013 0.84 -0.2 15,879 - Psest_0013 0.85 +1.5 15,879 - Psest_0013 0.85 +0.8 15,879 - Psest_0013 0.85 +2.4 15,879 - Psest_0013 0.85 +0.1 15,879 - Psest_0013 0.85 +0.3 15,879 - Psest_0013 0.85 +1.0 15,879 - Psest_0013 0.85 -1.8 15,879 - Psest_0013 0.85 -2.2 15,932 + +1.5 15,932 + -0.7 15,932 + +1.3 15,933 - -0.2 15,933 - -2.0 15,933 - -1.4 15,933 - -0.7 15,944 + +0.3 15,956 + -3.2 15,956 + -0.5 15,956 + +3.1 15,956 + -1.0 15,956 + +0.7 15,956 + +1.3 15,956 + -2.0 15,956 + -3.3 15,956 + +0.3 15,956 + +2.9 15,956 + +1.8 15,956 + +0.5 15,956 + -0.7 15,956 + +2.2 15,956 + -2.6 15,956 + -2.4 15,956 + +0.3 15,957 - -1.2 15,957 - +0.0 15,957 - -3.3 15,957 - +3.0 15,957 - +0.0 15,957 - +1.0 15,957 - +1.3 16,041 + -1.0 16,042 - +0.8 16,042 - +1.2 16,156 + Psest_0014 0.43 +1.9 16,156 + Psest_0014 0.43 +0.2 16,156 + Psest_0014 0.43 -1.0 16,156 + Psest_0014 0.43 -1.5 16,157 - Psest_0014 0.43 +1.7 16,181 + Psest_0014 0.52 +1.5 16,181 + Psest_0014 0.52 +0.9 16,182 - Psest_0014 0.52 -2.6 16,506 + Psest_0015 0.54 -0.5 16,575 + Psest_0015 0.76 -0.0 16,575 + Psest_0015 0.76 -4.3 16,575 + Psest_0015 0.76 -0.9 16,576 - Psest_0015 0.76 -1.1 16,698 + +0.7 16,699 - -0.7 16,733 + +0.1 16,734 - +1.1 16,734 - +1.1 16,734 - +0.6
Or see this region's nucleotide sequence