Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1277 and Psest_1278 are separated by 71 nucleotides Psest_1278 and Psest_1279 are separated by 19 nucleotides Psest_1279 and Psest_1280 are separated by 81 nucleotides
Psest_1277: Psest_1277 - 2-isopropylmalate synthase, yeast type, at 1,380,523 to 1,382,193
_1277
Psest_1278: Psest_1278 - hypothetical protein, at 1,382,265 to 1,382,438
_1278
Psest_1279: Psest_1279 - hypothetical protein, at 1,382,458 to 1,382,841
_1279
Psest_1280: Psest_1280 - TonB-dependent copper receptor, at 1,382,923 to 1,385,130
_1280
Position (kb)
1382
1383 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1381.471 kb on + strand, within Psest_1277 at 1381.472 kb on - strand, within Psest_1277 at 1381.523 kb on + strand, within Psest_1277 at 1381.523 kb on + strand, within Psest_1277 at 1381.523 kb on + strand, within Psest_1277 at 1381.523 kb on + strand, within Psest_1277 at 1381.523 kb on + strand, within Psest_1277 at 1381.524 kb on - strand, within Psest_1277 at 1381.524 kb on - strand, within Psest_1277 at 1381.524 kb on - strand, within Psest_1277 at 1381.524 kb on - strand, within Psest_1277 at 1381.528 kb on - strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1382.459 kb on + strand at 1382.460 kb on - strand at 1382.517 kb on + strand, within Psest_1279 at 1382.518 kb on - strand, within Psest_1279 at 1382.518 kb on - strand, within Psest_1279 at 1382.518 kb on - strand, within Psest_1279 at 1382.566 kb on + strand, within Psest_1279 at 1382.566 kb on + strand, within Psest_1279 at 1382.566 kb on + strand, within Psest_1279 at 1382.567 kb on - strand, within Psest_1279 at 1382.567 kb on - strand, within Psest_1279 at 1382.923 kb on - strand at 1382.935 kb on - strand at 1382.935 kb on - strand at 1383.117 kb on + strand at 1383.118 kb on - strand at 1383.209 kb on - strand, within Psest_1280 at 1383.240 kb on - strand, within Psest_1280 at 1383.364 kb on + strand, within Psest_1280 at 1383.364 kb on + strand, within Psest_1280 at 1383.364 kb on + strand, within Psest_1280 at 1383.364 kb on + strand, within Psest_1280 at 1383.364 kb on + strand, within Psest_1280 at 1383.365 kb on - strand, within Psest_1280 at 1383.365 kb on - strand, within Psest_1280 at 1383.365 kb on - strand, within Psest_1280 at 1383.365 kb on - strand, within Psest_1280 at 1383.365 kb on - strand, within Psest_1280 at 1383.616 kb on + strand, within Psest_1280 at 1383.616 kb on + strand, within Psest_1280 at 1383.617 kb on - strand, within Psest_1280 at 1383.666 kb on + strand, within Psest_1280 at 1383.739 kb on + strand, within Psest_1280 at 1383.748 kb on + strand, within Psest_1280 at 1383.749 kb on - strand, within Psest_1280 at 1383.835 kb on + strand, within Psest_1280 at 1383.835 kb on + strand, within Psest_1280 at 1383.835 kb on + strand, within Psest_1280 at 1383.835 kb on + strand, within Psest_1280 at 1383.836 kb on - strand, within Psest_1280
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 1,381,471 + Psest_1277 0.57 +0.5 1,381,472 - Psest_1277 0.57 +0.2 1,381,523 + Psest_1277 0.60 -0.5 1,381,523 + Psest_1277 0.60 +1.0 1,381,523 + Psest_1277 0.60 +1.5 1,381,523 + Psest_1277 0.60 -2.8 1,381,523 + Psest_1277 0.60 -2.8 1,381,524 - Psest_1277 0.60 -1.3 1,381,524 - Psest_1277 0.60 +2.4 1,381,524 - Psest_1277 0.60 +0.3 1,381,524 - Psest_1277 0.60 -0.2 1,381,528 - Psest_1277 0.60 +0.2 1,381,805 + Psest_1277 0.77 -1.8 1,381,805 + Psest_1277 0.77 +2.3 1,381,805 + Psest_1277 0.77 -1.2 1,381,805 + Psest_1277 0.77 +1.6 1,381,805 + Psest_1277 0.77 -2.8 1,381,805 + Psest_1277 0.77 -1.2 1,381,805 + Psest_1277 0.77 +2.3 1,381,805 + Psest_1277 0.77 -1.5 1,381,805 + Psest_1277 0.77 -0.8 1,381,805 + Psest_1277 0.77 +1.8 1,381,806 - Psest_1277 0.77 -0.7 1,381,806 - Psest_1277 0.77 -0.0 1,381,806 - Psest_1277 0.77 -1.1 1,381,806 - Psest_1277 0.77 -0.5 1,381,806 - Psest_1277 0.77 -1.2 1,381,806 - Psest_1277 0.77 +0.7 1,381,806 - Psest_1277 0.77 -0.4 1,381,806 - Psest_1277 0.77 -1.9 1,382,459 + +0.2 1,382,460 - -0.4 1,382,517 + Psest_1279 0.15 +0.5 1,382,518 - Psest_1279 0.16 -0.6 1,382,518 - Psest_1279 0.16 -1.9 1,382,518 - Psest_1279 0.16 +0.5 1,382,566 + Psest_1279 0.28 +0.1 1,382,566 + Psest_1279 0.28 +1.1 1,382,566 + Psest_1279 0.28 +1.0 1,382,567 - Psest_1279 0.28 +2.7 1,382,567 - Psest_1279 0.28 +1.2 1,382,923 - -0.5 1,382,935 - +1.8 1,382,935 - -1.7 1,383,117 + -1.4 1,383,118 - +0.6 1,383,209 - Psest_1280 0.13 -1.4 1,383,240 - Psest_1280 0.14 +0.8 1,383,364 + Psest_1280 0.20 -1.1 1,383,364 + Psest_1280 0.20 +0.2 1,383,364 + Psest_1280 0.20 -0.6 1,383,364 + Psest_1280 0.20 +1.1 1,383,364 + Psest_1280 0.20 -0.6 1,383,365 - Psest_1280 0.20 +0.8 1,383,365 - Psest_1280 0.20 -0.4 1,383,365 - Psest_1280 0.20 +0.7 1,383,365 - Psest_1280 0.20 +1.2 1,383,365 - Psest_1280 0.20 -1.8 1,383,616 + Psest_1280 0.31 +0.9 1,383,616 + Psest_1280 0.31 +1.1 1,383,617 - Psest_1280 0.31 +0.8 1,383,666 + Psest_1280 0.34 +0.2 1,383,739 + Psest_1280 0.37 +0.6 1,383,748 + Psest_1280 0.37 +2.2 1,383,749 - Psest_1280 0.37 +0.5 1,383,835 + Psest_1280 0.41 -1.6 1,383,835 + Psest_1280 0.41 +1.1 1,383,835 + Psest_1280 0.41 -1.0 1,383,835 + Psest_1280 0.41 -0.1 1,383,836 - Psest_1280 0.41 +1.5
Or see this region's nucleotide sequence