Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1113 and Psest_1114 are separated by 42 nucleotides Psest_1114 and Psest_1115 are separated by 21 nucleotides Psest_1115 and Psest_1116 overlap by 8 nucleotides Psest_1116 and Psest_1117 are separated by 84 nucleotides
Psest_1113: Psest_1113 - peptide chain release factor 1, at 1,207,815 to 1,208,897
_1113
Psest_1114: Psest_1114 - protein-(glutamine-N5) methyltransferase, release factor-specific, at 1,208,940 to 1,209,767
_1114
Psest_1115: Psest_1115 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2, at 1,209,789 to 1,210,538
_1115
Psest_1116: Psest_1116 - glutamate racemase, at 1,210,531 to 1,211,325
_1116
Psest_1117: Psest_1117 - Lipid A 3-O-deacylase (PagL)., at 1,211,410 to 1,211,928
_1117
Position (kb)
1209
1210
1211 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1208.902 kb on + strand at 1208.902 kb on + strand at 1209.927 kb on + strand, within Psest_1115 at 1209.927 kb on + strand, within Psest_1115 at 1209.974 kb on + strand, within Psest_1115 at 1210.340 kb on + strand, within Psest_1115 at 1211.395 kb on + strand at 1211.395 kb on + strand at 1211.397 kb on + strand at 1211.397 kb on + strand at 1211.397 kb on + strand at 1211.398 kb on - strand at 1211.398 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 1,208,902 + +0.8 1,208,902 + -2.6 1,209,927 + Psest_1115 0.18 +0.3 1,209,927 + Psest_1115 0.18 +1.0 1,209,974 + Psest_1115 0.25 +1.3 1,210,340 + Psest_1115 0.73 +3.2 1,211,395 + +1.4 1,211,395 + +0.5 1,211,397 + -0.1 1,211,397 + +0.5 1,211,397 + +1.0 1,211,398 - -1.6 1,211,398 - +1.2
Or see this region's nucleotide sequence