Experiment: Paraquat dichloride 0.015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0064 and Psest_0065 are separated by 271 nucleotides Psest_0065 and Psest_0066 are separated by 276 nucleotides Psest_0066 and Psest_0067 are separated by 8 nucleotides
Psest_0064: Psest_0064 - Transcriptional regulator, at 64,151 to 65,053
_0064
Psest_0065: Psest_0065 - Subtilisin-like serine proteases, at 65,325 to 66,716
_0065
Psest_0066: Psest_0066 - Protein of unknown function (DUF3060)., at 66,993 to 67,493
_0066
Psest_0067: Psest_0067 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 67,502 to 68,500
_0067
Position (kb)
65
66
67 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 64.829 kb on + strand, within Psest_0064 at 64.829 kb on + strand, within Psest_0064 at 64.829 kb on + strand, within Psest_0064 at 64.829 kb on + strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 65.099 kb on + strand at 65.099 kb on + strand at 65.179 kb on + strand at 65.246 kb on + strand at 65.246 kb on + strand at 65.247 kb on - strand at 65.247 kb on - strand at 65.247 kb on - strand at 65.269 kb on + strand at 65.269 kb on + strand at 65.269 kb on + strand at 65.270 kb on - strand at 65.270 kb on - strand at 65.270 kb on - strand at 65.270 kb on - strand at 65.683 kb on - strand, within Psest_0065 at 65.724 kb on - strand, within Psest_0065 at 65.769 kb on + strand, within Psest_0065 at 65.994 kb on + strand, within Psest_0065 at 66.162 kb on + strand, within Psest_0065 at 66.162 kb on + strand, within Psest_0065 at 66.162 kb on + strand, within Psest_0065 at 66.162 kb on + strand, within Psest_0065 at 66.162 kb on + strand, within Psest_0065 at 66.163 kb on - strand, within Psest_0065 at 66.163 kb on - strand, within Psest_0065 at 66.250 kb on - strand, within Psest_0065 at 66.250 kb on - strand, within Psest_0065 at 66.351 kb on + strand, within Psest_0065 at 66.351 kb on + strand, within Psest_0065 at 66.351 kb on + strand, within Psest_0065 at 66.352 kb on - strand, within Psest_0065 at 66.352 kb on - strand, within Psest_0065 at 66.352 kb on - strand, within Psest_0065 at 66.420 kb on + strand, within Psest_0065 at 66.421 kb on - strand, within Psest_0065 at 66.421 kb on - strand, within Psest_0065 at 66.507 kb on + strand, within Psest_0065 at 66.507 kb on + strand, within Psest_0065 at 66.507 kb on + strand, within Psest_0065 at 66.507 kb on + strand, within Psest_0065 at 66.508 kb on - strand, within Psest_0065 at 66.508 kb on - strand, within Psest_0065 at 66.508 kb on - strand, within Psest_0065 at 66.561 kb on + strand, within Psest_0065 at 66.562 kb on - strand, within Psest_0065 at 66.572 kb on - strand, within Psest_0065 at 66.652 kb on + strand at 66.719 kb on + strand at 66.719 kb on + strand at 66.719 kb on + strand at 66.719 kb on + strand at 66.720 kb on - strand at 66.720 kb on - strand at 66.723 kb on + strand at 66.724 kb on - strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.856 kb on + strand at 66.857 kb on - strand at 66.857 kb on - strand at 67.421 kb on + strand, within Psest_0066 at 67.421 kb on + strand, within Psest_0066 at 67.421 kb on + strand at 67.422 kb on - strand, within Psest_0066 at 67.422 kb on - strand, within Psest_0066 at 67.699 kb on - strand, within Psest_0067
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.015 mg/ml remove 64,829 + Psest_0064 0.75 +4.0 64,829 + Psest_0064 0.75 +1.2 64,829 + Psest_0064 0.75 +2.1 64,829 + Psest_0064 0.75 +1.2 64,830 - Psest_0064 0.75 +3.9 64,830 - Psest_0064 0.75 +2.7 64,830 - Psest_0064 0.75 +3.6 64,830 - Psest_0064 0.75 +1.2 64,830 - Psest_0064 0.75 -3.4 65,099 + +1.4 65,099 + -2.6 65,179 + -2.2 65,246 + -0.3 65,246 + -2.2 65,247 - +1.8 65,247 - +1.8 65,247 - +0.2 65,269 + -0.3 65,269 + -1.3 65,269 + +1.6 65,270 - +2.9 65,270 - -0.1 65,270 - -0.2 65,270 - +0.2 65,683 - Psest_0065 0.26 -0.1 65,724 - Psest_0065 0.29 -1.3 65,769 + Psest_0065 0.32 +0.5 65,994 + Psest_0065 0.48 -1.0 66,162 + Psest_0065 0.60 -1.8 66,162 + Psest_0065 0.60 +0.8 66,162 + Psest_0065 0.60 -0.8 66,162 + Psest_0065 0.60 +0.4 66,162 + Psest_0065 0.60 +2.1 66,163 - Psest_0065 0.60 +0.8 66,163 - Psest_0065 0.60 -0.5 66,250 - Psest_0065 0.66 +1.3 66,250 - Psest_0065 0.66 +0.9 66,351 + Psest_0065 0.74 +0.4 66,351 + Psest_0065 0.74 -1.5 66,351 + Psest_0065 0.74 -0.5 66,352 - Psest_0065 0.74 -0.2 66,352 - Psest_0065 0.74 -0.8 66,352 - Psest_0065 0.74 -1.5 66,420 + Psest_0065 0.79 +1.9 66,421 - Psest_0065 0.79 -2.4 66,421 - Psest_0065 0.79 +0.6 66,507 + Psest_0065 0.85 -1.0 66,507 + Psest_0065 0.85 +4.1 66,507 + Psest_0065 0.85 +2.7 66,507 + Psest_0065 0.85 +3.0 66,508 - Psest_0065 0.85 -0.8 66,508 - Psest_0065 0.85 -0.1 66,508 - Psest_0065 0.85 +0.3 66,561 + Psest_0065 0.89 -0.4 66,562 - Psest_0065 0.89 -1.2 66,572 - Psest_0065 0.90 -2.8 66,652 + -1.1 66,719 + -0.0 66,719 + -0.9 66,719 + +0.5 66,719 + +1.2 66,720 - -1.3 66,720 - -1.2 66,723 + +1.2 66,724 - -1.2 66,856 + +1.5 66,856 + +0.5 66,856 + +2.6 66,856 + -1.1 66,856 + -2.3 66,856 + -0.6 66,856 + -1.6 66,857 - -1.5 66,857 - -0.3 67,421 + Psest_0066 0.85 +1.7 67,421 + Psest_0066 0.85 +1.0 67,421 + +2.8 67,422 - Psest_0066 0.86 +0.3 67,422 - Psest_0066 0.86 -0.1 67,699 - Psest_0067 0.20 +0.5
Or see this region's nucleotide sequence