Experiment: Paraquat dichloride 0.015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0445 and Psest_0446 are separated by 110 nucleotides Psest_0446 and Psest_0447 are separated by 108 nucleotides Psest_0447 and Psest_0448 are separated by 24 nucleotides
Psest_0445: Psest_0445 - PAS domain S-box/diguanylate cyclase (GGDEF) domain, at 484,818 to 487,490
_0445
Psest_0446: Psest_0446 - hypothetical protein, at 487,601 to 488,128
_0446
Psest_0447: Psest_0447 - pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase, long form, at 488,237 to 490,240
_0447
Psest_0448: Psest_0448 - pyruvate dehydrogenase E1 component, homodimeric type, at 490,265 to 492,910
_0448
Position (kb) 488
489
490
491 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 487.248 kb on - strand at 487.479 kb on - strand at 487.482 kb on + strand at 487.482 kb on + strand at 487.482 kb on + strand at 487.483 kb on - strand at 487.483 kb on - strand at 487.483 kb on - strand at 487.510 kb on - strand at 487.510 kb on - strand at 487.510 kb on - strand at 487.571 kb on - strand at 487.575 kb on - strand at 487.581 kb on - strand at 487.635 kb on + strand at 487.636 kb on - strand at 487.721 kb on + strand, within Psest_0446 at 487.721 kb on + strand, within Psest_0446 at 487.722 kb on - strand, within Psest_0446 at 487.722 kb on - strand, within Psest_0446 at 487.746 kb on - strand, within Psest_0446 at 487.746 kb on - strand, within Psest_0446 at 487.746 kb on - strand, within Psest_0446 at 487.746 kb on - strand, within Psest_0446 at 487.789 kb on + strand, within Psest_0446 at 487.789 kb on + strand, within Psest_0446 at 487.790 kb on - strand, within Psest_0446 at 487.790 kb on - strand, within Psest_0446 at 487.805 kb on + strand, within Psest_0446 at 487.805 kb on + strand, within Psest_0446 at 487.805 kb on + strand, within Psest_0446 at 487.805 kb on + strand, within Psest_0446 at 487.805 kb on + strand, within Psest_0446 at 487.805 kb on + strand, within Psest_0446 at 487.805 kb on + strand, within Psest_0446 at 487.805 kb on + strand, within Psest_0446 at 487.806 kb on - strand, within Psest_0446 at 487.806 kb on - strand, within Psest_0446 at 487.806 kb on - strand, within Psest_0446 at 487.806 kb on - strand, within Psest_0446 at 487.806 kb on - strand, within Psest_0446 at 487.806 kb on - strand, within Psest_0446 at 487.806 kb on - strand, within Psest_0446 at 487.806 kb on - strand, within Psest_0446 at 487.824 kb on - strand, within Psest_0446 at 487.974 kb on - strand, within Psest_0446 at 487.983 kb on - strand, within Psest_0446 at 488.005 kb on + strand, within Psest_0446 at 488.252 kb on - strand at 490.244 kb on - strand at 490.244 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.015 mg/ml remove 487,248 - -2.4 487,479 - -1.0 487,482 + -0.7 487,482 + +3.8 487,482 + -1.0 487,483 - -0.4 487,483 - +0.2 487,483 - +1.4 487,510 - +1.1 487,510 - +0.3 487,510 - -1.1 487,571 - +0.4 487,575 - -0.7 487,581 - +0.9 487,635 + -0.4 487,636 - +0.7 487,721 + Psest_0446 0.23 +3.9 487,721 + Psest_0446 0.23 +1.9 487,722 - Psest_0446 0.23 +1.1 487,722 - Psest_0446 0.23 -1.3 487,746 - Psest_0446 0.27 +0.7 487,746 - Psest_0446 0.27 +0.3 487,746 - Psest_0446 0.27 +0.8 487,746 - Psest_0446 0.27 +0.7 487,789 + Psest_0446 0.36 +1.2 487,789 + Psest_0446 0.36 -1.7 487,790 - Psest_0446 0.36 -0.2 487,790 - Psest_0446 0.36 -0.2 487,805 + Psest_0446 0.39 -0.3 487,805 + Psest_0446 0.39 -1.8 487,805 + Psest_0446 0.39 +1.2 487,805 + Psest_0446 0.39 -1.1 487,805 + Psest_0446 0.39 -0.1 487,805 + Psest_0446 0.39 -0.2 487,805 + Psest_0446 0.39 -1.1 487,805 + Psest_0446 0.39 +2.0 487,806 - Psest_0446 0.39 +0.9 487,806 - Psest_0446 0.39 -0.3 487,806 - Psest_0446 0.39 +0.3 487,806 - Psest_0446 0.39 +0.6 487,806 - Psest_0446 0.39 -3.0 487,806 - Psest_0446 0.39 +0.9 487,806 - Psest_0446 0.39 +0.8 487,806 - Psest_0446 0.39 -1.4 487,824 - Psest_0446 0.42 +5.3 487,974 - Psest_0446 0.71 -0.4 487,983 - Psest_0446 0.72 +1.5 488,005 + Psest_0446 0.77 +0.2 488,252 - +0.6 490,244 - -0.3 490,244 - -0.1
Or see this region's nucleotide sequence