Experiment: Paraquat dichloride 0.015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2005 and Psest_2006 are separated by 191 nucleotides Psest_2006 and Psest_2007 are separated by 30 nucleotides Psest_2007 and Psest_2008 are separated by 256 nucleotides Psest_2008 and Psest_2009 are separated by 135 nucleotides Psest_2009 and Psest_2010 are separated by 564 nucleotides
Psest_2005: Psest_2005 - 16S rRNA, at 2,121,803 to 2,123,221
_2005
Psest_2006: Psest_2006 - tRNA_Ile_GAT, at 2,123,413 to 2,123,489
_2006
Psest_2007: Psest_2007 - tRNA_Ala_TGC, at 2,123,520 to 2,123,595
_2007
Psest_2008: Psest_2008 - 23S rRNA, at 2,123,852 to 2,126,738
_2008
Psest_2009: Psest_2009 - 5S rRNA, at 2,126,874 to 2,126,989
_2009
Psest_2010: Psest_2010 - queuosine biosynthesis protein QueD, at 2,127,554 to 2,127,925
_2010
Position (kb)
2123
2124
2125
2126
2127 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2127.180 kb on + strand at 2127.180 kb on + strand at 2127.180 kb on + strand at 2127.183 kb on + strand at 2127.183 kb on + strand at 2127.240 kb on - strand at 2127.258 kb on + strand at 2127.258 kb on + strand at 2127.258 kb on + strand at 2127.259 kb on - strand at 2127.275 kb on - strand at 2127.306 kb on + strand at 2127.307 kb on - strand at 2127.338 kb on - strand at 2127.342 kb on - strand at 2127.343 kb on + strand at 2127.343 kb on + strand at 2127.344 kb on - strand at 2127.378 kb on + strand at 2127.379 kb on - strand at 2127.379 kb on - strand at 2127.380 kb on + strand at 2127.380 kb on + strand at 2127.452 kb on + strand at 2127.452 kb on + strand at 2127.456 kb on + strand at 2127.456 kb on + strand at 2127.456 kb on + strand at 2127.457 kb on - strand at 2127.525 kb on + strand at 2127.525 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.015 mg/ml remove 2,127,180 + +1.7 2,127,180 + -0.2 2,127,180 + -3.2 2,127,183 + -1.6 2,127,183 + +1.2 2,127,240 - -2.8 2,127,258 + -1.8 2,127,258 + +0.4 2,127,258 + -1.0 2,127,259 - +0.8 2,127,275 - -0.0 2,127,306 + -0.5 2,127,307 - -0.8 2,127,338 - +0.2 2,127,342 - -2.4 2,127,343 + +0.5 2,127,343 + +0.9 2,127,344 - -1.2 2,127,378 + +0.3 2,127,379 - -1.4 2,127,379 - +0.0 2,127,380 + -0.1 2,127,380 + +0.7 2,127,452 + -3.2 2,127,452 + +1.0 2,127,456 + +0.9 2,127,456 + -1.4 2,127,456 + -0.4 2,127,457 - +0.1 2,127,525 + +0.9 2,127,525 + -1.2
Or see this region's nucleotide sequence