Experiment: Fusidic 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3959 and Psest_3960 are separated by 65 nucleotides Psest_3960 and Psest_3961 overlap by 4 nucleotides Psest_3961 and Psest_3962 are separated by 117 nucleotides Psest_3962 and Psest_3963 are separated by 169 nucleotides
Psest_3959: Psest_3959 - thioesterase domain, putative, at 4,126,342 to 4,126,794
_3959
Psest_3960: Psest_3960 - 3'(2'),5'-bisphosphate nucleotidase, bacterial, at 4,126,860 to 4,127,678
_3960
Psest_3961: Psest_3961 - NTP pyrophosphohydrolases including oxidative damage repair enzymes, at 4,127,675 to 4,128,241
_3961
Psest_3962: Psest_3962 - haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED, at 4,128,359 to 4,129,039
_3962
Psest_3963: Psest_3963 - PAS domain S-box, at 4,129,209 to 4,131,770
_3963
Position (kb)
4127
4128
4129 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4126.792 kb on + strand at 4126.793 kb on - strand at 4126.793 kb on - strand at 4126.793 kb on - strand at 4126.839 kb on + strand at 4126.839 kb on + strand at 4126.840 kb on - strand at 4126.940 kb on - strand at 4126.940 kb on - strand at 4126.940 kb on - strand at 4126.940 kb on - strand at 4127.299 kb on + strand, within Psest_3960 at 4127.299 kb on + strand, within Psest_3960 at 4127.299 kb on + strand, within Psest_3960 at 4127.666 kb on - strand at 4127.666 kb on - strand at 4127.721 kb on + strand at 4127.722 kb on - strand at 4127.722 kb on - strand at 4128.024 kb on + strand, within Psest_3961 at 4128.025 kb on - strand, within Psest_3961 at 4128.045 kb on + strand, within Psest_3961 at 4128.045 kb on + strand, within Psest_3961 at 4128.045 kb on + strand, within Psest_3961 at 4128.046 kb on - strand, within Psest_3961 at 4128.046 kb on - strand, within Psest_3961 at 4128.046 kb on - strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.135 kb on + strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.136 kb on - strand, within Psest_3961 at 4128.221 kb on + strand at 4128.221 kb on + strand at 4128.221 kb on + strand at 4128.221 kb on + strand at 4128.221 kb on + strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.222 kb on - strand at 4128.273 kb on + strand at 4128.273 kb on + strand at 4128.284 kb on - strand at 4128.573 kb on - strand, within Psest_3962 at 4128.704 kb on - strand, within Psest_3962 at 4129.072 kb on + strand at 4129.072 kb on + strand at 4129.072 kb on + strand at 4129.072 kb on + strand at 4129.072 kb on + strand at 4129.072 kb on + strand at 4129.073 kb on - strand at 4129.073 kb on - strand at 4129.073 kb on - strand at 4129.073 kb on - strand at 4129.073 kb on - strand at 4129.073 kb on - strand at 4129.073 kb on - strand at 4129.077 kb on + strand at 4129.077 kb on + strand at 4129.077 kb on + strand at 4129.078 kb on - strand at 4129.078 kb on - strand at 4129.078 kb on - strand at 4129.078 kb on - strand at 4129.176 kb on + strand at 4129.177 kb on - strand at 4129.198 kb on + strand at 4129.199 kb on - strand at 4129.219 kb on + strand at 4129.220 kb on - strand at 4129.220 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.016 mg/ml remove 4,126,792 + +0.0 4,126,793 - +0.6 4,126,793 - +0.6 4,126,793 - +1.7 4,126,839 + +0.1 4,126,839 + +0.3 4,126,840 - +0.9 4,126,940 - -0.4 4,126,940 - +0.1 4,126,940 - +1.7 4,126,940 - -0.2 4,127,299 + Psest_3960 0.54 +0.4 4,127,299 + Psest_3960 0.54 +1.6 4,127,299 + Psest_3960 0.54 +0.9 4,127,666 - +0.4 4,127,666 - +2.4 4,127,721 + +1.1 4,127,722 - -1.8 4,127,722 - +0.4 4,128,024 + Psest_3961 0.62 +1.2 4,128,025 - Psest_3961 0.62 -0.7 4,128,045 + Psest_3961 0.65 +4.1 4,128,045 + Psest_3961 0.65 -0.5 4,128,045 + Psest_3961 0.65 -0.1 4,128,046 - Psest_3961 0.65 +0.2 4,128,046 - Psest_3961 0.65 +0.1 4,128,046 - Psest_3961 0.65 +2.2 4,128,135 + Psest_3961 0.81 +1.5 4,128,135 + Psest_3961 0.81 +0.6 4,128,135 + Psest_3961 0.81 +0.1 4,128,135 + Psest_3961 0.81 -1.1 4,128,135 + Psest_3961 0.81 +0.5 4,128,135 + Psest_3961 0.81 +2.1 4,128,135 + Psest_3961 0.81 -1.4 4,128,135 + Psest_3961 0.81 +2.2 4,128,135 + Psest_3961 0.81 +0.3 4,128,135 + Psest_3961 0.81 +0.1 4,128,136 - Psest_3961 0.81 -0.3 4,128,136 - Psest_3961 0.81 +0.4 4,128,136 - Psest_3961 0.81 +1.5 4,128,136 - Psest_3961 0.81 -2.6 4,128,136 - Psest_3961 0.81 +0.3 4,128,136 - Psest_3961 0.81 +0.6 4,128,136 - Psest_3961 0.81 +1.6 4,128,136 - Psest_3961 0.81 +0.1 4,128,136 - Psest_3961 0.81 +0.2 4,128,136 - Psest_3961 0.81 +0.1 4,128,136 - Psest_3961 0.81 +1.7 4,128,136 - Psest_3961 0.81 -0.1 4,128,221 + -0.4 4,128,221 + -1.2 4,128,221 + +0.1 4,128,221 + +0.1 4,128,221 + +2.4 4,128,222 - -0.2 4,128,222 - +0.6 4,128,222 - +0.6 4,128,222 - +0.3 4,128,222 - -1.5 4,128,222 - +1.6 4,128,222 - -0.2 4,128,222 - +0.4 4,128,222 - -0.3 4,128,273 + +1.3 4,128,273 + -0.3 4,128,284 - -1.2 4,128,573 - Psest_3962 0.31 -0.2 4,128,704 - Psest_3962 0.51 +0.4 4,129,072 + -1.0 4,129,072 + +0.6 4,129,072 + +1.4 4,129,072 + -0.2 4,129,072 + -0.8 4,129,072 + -1.9 4,129,073 - +0.5 4,129,073 - +0.2 4,129,073 - +0.8 4,129,073 - +1.5 4,129,073 - +0.1 4,129,073 - +1.0 4,129,073 - +0.6 4,129,077 + -1.5 4,129,077 + -0.9 4,129,077 + -1.0 4,129,078 - -0.4 4,129,078 - -0.2 4,129,078 - -2.2 4,129,078 - +1.6 4,129,176 + +1.2 4,129,177 - -1.0 4,129,198 + +0.1 4,129,199 - +0.9 4,129,219 + -1.5 4,129,220 - -0.6 4,129,220 - +1.9
Or see this region's nucleotide sequence