Experiment: Fusidic 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1381 and Psest_1382 overlap by 4 nucleotides Psest_1382 and Psest_1383 are separated by 104 nucleotides
Psest_1381: Psest_1381 - Predicted alpha/beta hydrolase, at 1,491,207 to 1,492,163
_1381
Psest_1382: Psest_1382 - Transcription elongation factor, at 1,492,160 to 1,492,636
_1382
Psest_1383: Psest_1383 - DNA topoisomerase III, bacteria and conjugative plasmid, at 1,492,741 to 1,494,681
_1383
Position (kb)
1492
1493 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1491.245 kb on + strand at 1491.245 kb on + strand at 1491.245 kb on + strand at 1491.245 kb on + strand at 1491.245 kb on + strand at 1491.245 kb on + strand at 1491.245 kb on + strand at 1491.245 kb on + strand at 1491.245 kb on + strand at 1491.245 kb on + strand at 1491.246 kb on - strand at 1491.246 kb on - strand at 1491.246 kb on - strand at 1491.246 kb on - strand at 1491.246 kb on - strand at 1491.385 kb on - strand, within Psest_1381 at 1491.486 kb on + strand, within Psest_1381 at 1491.487 kb on - strand, within Psest_1381 at 1491.533 kb on + strand, within Psest_1381 at 1491.609 kb on + strand, within Psest_1381 at 1491.609 kb on + strand, within Psest_1381 at 1491.610 kb on - strand, within Psest_1381 at 1491.610 kb on - strand, within Psest_1381 at 1491.616 kb on - strand, within Psest_1381 at 1491.616 kb on - strand, within Psest_1381 at 1491.628 kb on - strand, within Psest_1381 at 1491.628 kb on - strand, within Psest_1381 at 1491.654 kb on + strand, within Psest_1381 at 1491.654 kb on + strand, within Psest_1381 at 1491.703 kb on - strand, within Psest_1381 at 1492.146 kb on + strand at 1492.147 kb on - strand at 1492.147 kb on - strand at 1492.148 kb on + strand at 1492.149 kb on - strand at 1492.149 kb on - strand at 1492.149 kb on - strand at 1492.149 kb on - strand at 1492.149 kb on - strand at 1492.286 kb on + strand, within Psest_1382 at 1492.286 kb on + strand, within Psest_1382 at 1492.286 kb on + strand, within Psest_1382 at 1492.287 kb on - strand, within Psest_1382 at 1492.287 kb on - strand, within Psest_1382 at 1492.287 kb on - strand, within Psest_1382 at 1492.613 kb on + strand at 1492.613 kb on + strand at 1492.613 kb on + strand at 1492.613 kb on + strand at 1492.614 kb on - strand at 1492.634 kb on + strand at 1492.650 kb on - strand at 1493.271 kb on - strand, within Psest_1383 at 1493.444 kb on + strand, within Psest_1383 at 1493.444 kb on + strand, within Psest_1383 at 1493.495 kb on + strand, within Psest_1383 at 1493.495 kb on + strand, within Psest_1383
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.016 mg/ml remove 1,491,245 + +1.3 1,491,245 + -0.7 1,491,245 + -1.6 1,491,245 + +1.9 1,491,245 + -1.0 1,491,245 + -0.0 1,491,245 + -0.0 1,491,245 + -0.1 1,491,245 + +1.0 1,491,245 + +0.2 1,491,246 - +1.0 1,491,246 - -4.1 1,491,246 - +0.2 1,491,246 - +0.3 1,491,246 - -0.2 1,491,385 - Psest_1381 0.19 -0.6 1,491,486 + Psest_1381 0.29 -1.8 1,491,487 - Psest_1381 0.29 +0.3 1,491,533 + Psest_1381 0.34 -1.0 1,491,609 + Psest_1381 0.42 -0.3 1,491,609 + Psest_1381 0.42 +2.1 1,491,610 - Psest_1381 0.42 -0.9 1,491,610 - Psest_1381 0.42 +2.2 1,491,616 - Psest_1381 0.43 +0.6 1,491,616 - Psest_1381 0.43 -0.5 1,491,628 - Psest_1381 0.44 +0.0 1,491,628 - Psest_1381 0.44 +0.6 1,491,654 + Psest_1381 0.47 -0.6 1,491,654 + Psest_1381 0.47 +1.8 1,491,703 - Psest_1381 0.52 -0.4 1,492,146 + +0.1 1,492,147 - -1.0 1,492,147 - -1.0 1,492,148 + -0.8 1,492,149 - -2.0 1,492,149 - +1.4 1,492,149 - +1.0 1,492,149 - -0.8 1,492,149 - +0.2 1,492,286 + Psest_1382 0.26 -0.4 1,492,286 + Psest_1382 0.26 +1.0 1,492,286 + Psest_1382 0.26 +0.8 1,492,287 - Psest_1382 0.27 -1.8 1,492,287 - Psest_1382 0.27 +0.0 1,492,287 - Psest_1382 0.27 +2.5 1,492,613 + -0.6 1,492,613 + -0.1 1,492,613 + -0.5 1,492,613 + +0.4 1,492,614 - +0.5 1,492,634 + +1.1 1,492,650 - -1.2 1,493,271 - Psest_1383 0.27 -0.1 1,493,444 + Psest_1383 0.36 +0.4 1,493,444 + Psest_1383 0.36 +0.4 1,493,495 + Psest_1383 0.39 -1.2 1,493,495 + Psest_1383 0.39 +1.0
Or see this region's nucleotide sequence