Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0664 and Psest_0665 are separated by 84 nucleotides Psest_0665 and Psest_0666 are separated by 105 nucleotides Psest_0666 and Psest_0667 are separated by 147 nucleotides
Psest_0664: Psest_0664 - diguanylate cyclase (GGDEF) domain, at 723,162 to 724,652
_0664
Psest_0665: Psest_0665 - Inorganic pyrophosphatase, at 724,737 to 725,264
_0665
Psest_0666: Psest_0666 - Uncharacterized protein conserved in bacteria, at 725,370 to 726,182
_0666
Psest_0667: Psest_0667 - Phosphodiesterase/alkaline phosphatase D, at 726,330 to 727,883
_0667
Position (kb) 725
726
727 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 724.490 kb on - strand, within Psest_0664 at 724.548 kb on + strand at 724.548 kb on + strand at 724.549 kb on - strand at 724.576 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.611 kb on + strand at 724.611 kb on + strand at 724.738 kb on + strand at 724.738 kb on + strand at 724.738 kb on + strand at 724.739 kb on - strand at 724.739 kb on - strand at 725.326 kb on - strand at 725.371 kb on + strand at 725.372 kb on - strand at 725.461 kb on + strand, within Psest_0666 at 725.462 kb on - strand, within Psest_0666 at 725.475 kb on + strand, within Psest_0666 at 725.475 kb on + strand, within Psest_0666 at 725.476 kb on - strand, within Psest_0666 at 725.476 kb on - strand, within Psest_0666 at 725.509 kb on + strand, within Psest_0666 at 725.509 kb on + strand, within Psest_0666 at 725.510 kb on - strand, within Psest_0666 at 725.614 kb on + strand, within Psest_0666 at 725.737 kb on + strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.034 kb on - strand, within Psest_0666 at 726.034 kb on - strand, within Psest_0666 at 726.216 kb on + strand at 726.216 kb on + strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.250 kb on + strand at 726.251 kb on - strand at 726.293 kb on - strand at 726.822 kb on + strand, within Psest_0667 at 726.822 kb on + strand, within Psest_0667 at 726.822 kb on + strand, within Psest_0667 at 726.823 kb on - strand, within Psest_0667 at 726.823 kb on - strand, within Psest_0667 at 726.823 kb on - strand, within Psest_0667 at 726.823 kb on - strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.880 kb on - strand, within Psest_0667 at 726.880 kb on - strand, within Psest_0667 at 726.880 kb on - strand, within Psest_0667 at 726.996 kb on + strand, within Psest_0667 at 726.996 kb on + strand, within Psest_0667 at 726.997 kb on - strand, within Psest_0667 at 727.002 kb on + strand, within Psest_0667
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 724,490 - Psest_0664 0.89 -1.3 724,548 + +0.6 724,548 + -2.5 724,549 - +0.7 724,576 + +0.5 724,578 + -0.2 724,578 + -0.7 724,578 + -0.8 724,578 + -1.1 724,578 + -0.5 724,579 - +0.5 724,579 - -1.1 724,579 - +0.8 724,579 - -1.1 724,579 - +0.2 724,611 + -0.9 724,611 + -2.3 724,738 + -0.3 724,738 + -1.2 724,738 + +0.1 724,739 - -0.2 724,739 - +0.4 725,326 - +0.4 725,371 + -0.3 725,372 - -2.7 725,461 + Psest_0666 0.11 -0.2 725,462 - Psest_0666 0.11 -1.0 725,475 + Psest_0666 0.13 +1.1 725,475 + Psest_0666 0.13 -0.3 725,476 - Psest_0666 0.13 +0.4 725,476 - Psest_0666 0.13 -2.1 725,509 + Psest_0666 0.17 -1.6 725,509 + Psest_0666 0.17 -0.7 725,510 - Psest_0666 0.17 -0.6 725,614 + Psest_0666 0.30 -1.4 725,737 + Psest_0666 0.45 -0.5 725,738 - Psest_0666 0.45 -0.5 725,738 - Psest_0666 0.45 -0.3 725,738 - Psest_0666 0.45 -0.1 725,738 - Psest_0666 0.45 -1.5 725,738 - Psest_0666 0.45 +0.9 725,738 - Psest_0666 0.45 -0.4 725,738 - Psest_0666 0.45 -1.6 726,033 + Psest_0666 0.82 -0.9 726,033 + Psest_0666 0.82 -0.5 726,033 + Psest_0666 0.82 -0.0 726,033 + Psest_0666 0.82 -0.6 726,033 + Psest_0666 0.82 +1.0 726,034 - Psest_0666 0.82 -1.5 726,034 - Psest_0666 0.82 -0.6 726,216 + +2.0 726,216 + +0.4 726,217 - -1.1 726,217 - +2.2 726,217 - -1.5 726,217 - -1.2 726,217 - -0.1 726,250 + -0.9 726,251 - -0.6 726,293 - -1.0 726,822 + Psest_0667 0.32 +0.8 726,822 + Psest_0667 0.32 +1.6 726,822 + Psest_0667 0.32 +1.2 726,823 - Psest_0667 0.32 -1.4 726,823 - Psest_0667 0.32 +0.3 726,823 - Psest_0667 0.32 -0.1 726,823 - Psest_0667 0.32 +0.2 726,879 + Psest_0667 0.35 -1.6 726,879 + Psest_0667 0.35 -0.6 726,879 + Psest_0667 0.35 -1.8 726,879 + Psest_0667 0.35 +0.1 726,879 + Psest_0667 0.35 -0.5 726,879 + Psest_0667 0.35 -0.1 726,879 + Psest_0667 0.35 +2.9 726,879 + Psest_0667 0.35 -1.0 726,880 - Psest_0667 0.35 -0.2 726,880 - Psest_0667 0.35 -1.3 726,880 - Psest_0667 0.35 -1.6 726,996 + Psest_0667 0.43 -1.0 726,996 + Psest_0667 0.43 +0.6 726,997 - Psest_0667 0.43 +1.0 727,002 + Psest_0667 0.43 +0.7
Or see this region's nucleotide sequence