Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0529 and Psest_0530 are separated by 9 nucleotides Psest_0530 and Psest_0531 overlap by 8 nucleotides Psest_0531 and Psest_0532 overlap by 41 nucleotides
Psest_0529: Psest_0529 - Putative Mg2+ and Co2+ transporter CorC, at 582,835 to 583,677
_0529
Psest_0530: Psest_0530 - metalloprotein, YbeY/UPF0054 family, at 583,687 to 584,169
_0530
Psest_0531: Psest_0531 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 584,162 to 585,328
_0531
Psest_0532: Psest_0532 - tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase enzyme MiaB, at 585,288 to 586,601
_0532
Position (kb) 584
585
586 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 583.489 kb on + strand, within Psest_0529 at 583.489 kb on + strand, within Psest_0529 at 583.490 kb on - strand, within Psest_0529 at 583.490 kb on - strand, within Psest_0529 at 583.586 kb on - strand, within Psest_0529 at 583.586 kb on - strand, within Psest_0529 at 583.730 kb on + strand at 583.730 kb on + strand at 583.730 kb on + strand at 583.730 kb on + strand at 583.730 kb on + strand at 583.730 kb on + strand at 584.230 kb on - strand at 584.267 kb on + strand at 584.267 kb on + strand at 584.267 kb on + strand at 584.268 kb on - strand at 584.268 kb on - strand at 584.318 kb on + strand, within Psest_0531 at 584.319 kb on - strand, within Psest_0531 at 584.506 kb on - strand, within Psest_0531 at 584.506 kb on - strand, within Psest_0531 at 584.506 kb on - strand, within Psest_0531 at 584.506 kb on - strand, within Psest_0531 at 584.557 kb on - strand, within Psest_0531 at 584.557 kb on - strand, within Psest_0531 at 584.557 kb on - strand, within Psest_0531 at 584.590 kb on - strand, within Psest_0531 at 584.590 kb on - strand, within Psest_0531 at 584.590 kb on - strand, within Psest_0531 at 584.676 kb on - strand, within Psest_0531 at 584.676 kb on - strand, within Psest_0531 at 584.693 kb on + strand, within Psest_0531 at 584.694 kb on - strand, within Psest_0531 at 584.717 kb on + strand, within Psest_0531 at 584.717 kb on + strand, within Psest_0531 at 584.718 kb on - strand, within Psest_0531 at 584.740 kb on - strand, within Psest_0531 at 584.740 kb on - strand, within Psest_0531 at 584.806 kb on - strand, within Psest_0531 at 584.806 kb on - strand, within Psest_0531 at 584.806 kb on - strand, within Psest_0531 at 584.917 kb on - strand, within Psest_0531 at 584.917 kb on - strand, within Psest_0531 at 585.156 kb on - strand, within Psest_0531 at 585.156 kb on - strand, within Psest_0531 at 585.156 kb on - strand, within Psest_0531 at 585.177 kb on - strand, within Psest_0531 at 585.214 kb on + strand at 585.760 kb on + strand, within Psest_0532 at 585.760 kb on + strand, within Psest_0532 at 585.760 kb on + strand, within Psest_0532 at 585.760 kb on + strand, within Psest_0532 at 585.760 kb on + strand, within Psest_0532 at 585.761 kb on - strand, within Psest_0532 at 585.761 kb on - strand, within Psest_0532 at 585.761 kb on - strand, within Psest_0532 at 585.819 kb on + strand, within Psest_0532 at 585.820 kb on - strand, within Psest_0532 at 585.835 kb on + strand, within Psest_0532 at 585.835 kb on + strand, within Psest_0532 at 585.835 kb on + strand, within Psest_0532 at 585.835 kb on + strand, within Psest_0532 at 585.835 kb on + strand, within Psest_0532 at 585.836 kb on - strand, within Psest_0532 at 585.836 kb on - strand, within Psest_0532 at 586.100 kb on - strand, within Psest_0532 at 586.100 kb on - strand, within Psest_0532 at 586.123 kb on + strand, within Psest_0532 at 586.123 kb on + strand, within Psest_0532 at 586.124 kb on - strand, within Psest_0532 at 586.124 kb on - strand, within Psest_0532 at 586.124 kb on - strand, within Psest_0532 at 586.124 kb on - strand, within Psest_0532
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 583,489 + Psest_0529 0.78 +0.7 583,489 + Psest_0529 0.78 -2.4 583,490 - Psest_0529 0.78 -1.6 583,490 - Psest_0529 0.78 -0.4 583,586 - Psest_0529 0.89 +0.7 583,586 - Psest_0529 0.89 +0.1 583,730 + -1.6 583,730 + +0.1 583,730 + +1.0 583,730 + -0.6 583,730 + +0.3 583,730 + +0.5 584,230 - -0.4 584,267 + +0.6 584,267 + -1.0 584,267 + +0.6 584,268 - -2.5 584,268 - +1.0 584,318 + Psest_0531 0.13 -1.6 584,319 - Psest_0531 0.13 +0.1 584,506 - Psest_0531 0.29 -1.6 584,506 - Psest_0531 0.29 -2.9 584,506 - Psest_0531 0.29 +0.7 584,506 - Psest_0531 0.29 -0.8 584,557 - Psest_0531 0.34 -0.5 584,557 - Psest_0531 0.34 -1.3 584,557 - Psest_0531 0.34 +1.4 584,590 - Psest_0531 0.37 -0.1 584,590 - Psest_0531 0.37 -3.3 584,590 - Psest_0531 0.37 -0.5 584,676 - Psest_0531 0.44 -1.1 584,676 - Psest_0531 0.44 -1.4 584,693 + Psest_0531 0.46 -0.0 584,694 - Psest_0531 0.46 -1.9 584,717 + Psest_0531 0.48 -1.4 584,717 + Psest_0531 0.48 -0.7 584,718 - Psest_0531 0.48 -2.5 584,740 - Psest_0531 0.50 +0.4 584,740 - Psest_0531 0.50 -2.5 584,806 - Psest_0531 0.55 -2.0 584,806 - Psest_0531 0.55 -0.2 584,806 - Psest_0531 0.55 +0.4 584,917 - Psest_0531 0.65 -0.6 584,917 - Psest_0531 0.65 -1.0 585,156 - Psest_0531 0.85 -2.7 585,156 - Psest_0531 0.85 -1.6 585,156 - Psest_0531 0.85 +1.6 585,177 - Psest_0531 0.87 -1.5 585,214 + -1.2 585,760 + Psest_0532 0.36 +0.9 585,760 + Psest_0532 0.36 +1.4 585,760 + Psest_0532 0.36 -0.7 585,760 + Psest_0532 0.36 +0.3 585,760 + Psest_0532 0.36 -2.3 585,761 - Psest_0532 0.36 +0.2 585,761 - Psest_0532 0.36 -0.7 585,761 - Psest_0532 0.36 -0.7 585,819 + Psest_0532 0.40 +0.3 585,820 - Psest_0532 0.40 -1.3 585,835 + Psest_0532 0.42 -1.4 585,835 + Psest_0532 0.42 +1.6 585,835 + Psest_0532 0.42 -0.2 585,835 + Psest_0532 0.42 -0.4 585,835 + Psest_0532 0.42 -1.8 585,836 - Psest_0532 0.42 -0.8 585,836 - Psest_0532 0.42 +2.8 586,100 - Psest_0532 0.62 +0.2 586,100 - Psest_0532 0.62 -0.8 586,123 + Psest_0532 0.64 +0.3 586,123 + Psest_0532 0.64 -2.5 586,124 - Psest_0532 0.64 -0.2 586,124 - Psest_0532 0.64 +2.0 586,124 - Psest_0532 0.64 +0.6 586,124 - Psest_0532 0.64 +0.4
Or see this region's nucleotide sequence