Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0449 and Psest_0450 are separated by 50 nucleotides Psest_0450 and Psest_0451 are separated by 78 nucleotides
Psest_0449: Psest_0449 - Glutamine synthetase adenylyltransferase, at 493,176 to 496,121
_0449
Psest_0450: Psest_0450 - Branched-chain amino acid aminotransferase (EC 2.6.1.42) (from data) , at 496,172 to 497,095
_0450
Psest_0451: Psest_0451 - lipopolysaccharide heptosyltransferase II, at 497,174 to 498,208
_0451
Position (kb)
496
497
498 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 495.177 kb on + strand, within Psest_0449 at 495.492 kb on + strand, within Psest_0449 at 495.492 kb on + strand, within Psest_0449 at 495.492 kb on + strand, within Psest_0449 at 495.492 kb on + strand, within Psest_0449 at 495.492 kb on + strand, within Psest_0449 at 495.493 kb on - strand, within Psest_0449 at 495.493 kb on - strand, within Psest_0449 at 495.854 kb on + strand at 495.854 kb on + strand at 495.864 kb on + strand at 495.909 kb on + strand at 495.910 kb on - strand at 495.911 kb on + strand at 495.911 kb on + strand at 495.993 kb on + strand at 496.094 kb on + strand at 496.094 kb on + strand at 496.094 kb on + strand at 496.094 kb on + strand at 496.268 kb on + strand, within Psest_0450 at 496.460 kb on + strand, within Psest_0450 at 496.460 kb on + strand, within Psest_0450 at 496.481 kb on + strand, within Psest_0450 at 496.481 kb on + strand, within Psest_0450 at 496.559 kb on + strand, within Psest_0450 at 496.559 kb on + strand, within Psest_0450 at 496.664 kb on + strand, within Psest_0450 at 496.664 kb on + strand, within Psest_0450 at 496.664 kb on + strand, within Psest_0450 at 496.664 kb on + strand, within Psest_0450 at 496.664 kb on + strand, within Psest_0450 at 496.664 kb on + strand, within Psest_0450 at 496.742 kb on + strand, within Psest_0450 at 496.796 kb on + strand, within Psest_0450 at 496.796 kb on + strand, within Psest_0450 at 496.796 kb on + strand, within Psest_0450 at 496.796 kb on + strand, within Psest_0450 at 496.796 kb on + strand, within Psest_0450 at 496.796 kb on + strand, within Psest_0450 at 496.878 kb on + strand, within Psest_0450 at 496.878 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 497.033 kb on + strand at 497.033 kb on + strand at 497.033 kb on + strand at 497.093 kb on + strand at 497.093 kb on + strand at 497.093 kb on + strand at 497.093 kb on + strand at 497.093 kb on + strand at 497.159 kb on + strand at 497.166 kb on + strand at 497.166 kb on + strand at 497.166 kb on + strand at 497.173 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 495,177 + Psest_0449 0.68 +0.6 495,492 + Psest_0449 0.79 -1.5 495,492 + Psest_0449 0.79 -1.0 495,492 + Psest_0449 0.79 -3.9 495,492 + Psest_0449 0.79 -1.6 495,492 + Psest_0449 0.79 -2.6 495,493 - Psest_0449 0.79 -0.1 495,493 - Psest_0449 0.79 +0.0 495,854 + +0.3 495,854 + -0.3 495,864 + +1.8 495,909 + -1.4 495,910 - +1.6 495,911 + +2.6 495,911 + -0.1 495,993 + -0.8 496,094 + -1.2 496,094 + -1.2 496,094 + -1.9 496,094 + +0.2 496,268 + Psest_0450 0.10 +4.4 496,460 + Psest_0450 0.31 +4.5 496,460 + Psest_0450 0.31 +1.2 496,481 + Psest_0450 0.33 +1.2 496,481 + Psest_0450 0.33 +1.9 496,559 + Psest_0450 0.42 +0.8 496,559 + Psest_0450 0.42 +3.0 496,664 + Psest_0450 0.53 +0.6 496,664 + Psest_0450 0.53 +7.0 496,664 + Psest_0450 0.53 +2.5 496,664 + Psest_0450 0.53 +2.6 496,664 + Psest_0450 0.53 +1.7 496,664 + Psest_0450 0.53 +4.6 496,742 + Psest_0450 0.62 +1.7 496,796 + Psest_0450 0.68 +1.4 496,796 + Psest_0450 0.68 +1.0 496,796 + Psest_0450 0.68 +1.2 496,796 + Psest_0450 0.68 +2.4 496,796 + Psest_0450 0.68 +1.5 496,796 + Psest_0450 0.68 +2.6 496,878 + Psest_0450 0.76 +2.0 496,878 + Psest_0450 0.76 +1.2 496,910 + Psest_0450 0.80 +2.2 496,910 + Psest_0450 0.80 +0.0 496,910 + Psest_0450 0.80 +0.7 496,910 + Psest_0450 0.80 +2.8 496,910 + Psest_0450 0.80 +1.3 496,910 + Psest_0450 0.80 +0.0 496,910 + Psest_0450 0.80 +0.2 496,910 + Psest_0450 0.80 -1.0 496,910 + Psest_0450 0.80 +0.3 497,033 + -0.9 497,033 + +1.5 497,033 + -0.4 497,093 + -0.0 497,093 + -1.6 497,093 + -0.7 497,093 + +0.0 497,093 + +0.5 497,159 + -0.9 497,166 + +0.6 497,166 + -1.1 497,166 + -0.8 497,173 + -2.2
Or see this region's nucleotide sequence