Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3302 and Psest_3303 are separated by 13 nucleotides Psest_3303 and Psest_3304 overlap by 8 nucleotides Psest_3304 and Psest_3305 are separated by 11 nucleotides Psest_3305 and Psest_3306 overlap by 1 nucleotides Psest_3306 and Psest_3307 overlap by 8 nucleotides
Psest_3302: Psest_3302 - Predicted membrane protein, at 3,469,392 to 3,469,883
_3302
Psest_3303: Psest_3303 - Anti-anti-sigma regulatory factor (antagonist of anti-sigma factor), at 3,469,897 to 3,470,211
_3303
Psest_3304: Psest_3304 - ABC-type transport system involved in resistance to organic solvents, auxiliary component, at 3,470,204 to 3,470,851
_3304
Psest_3305: Psest_3305 - ABC-type transport system involved in resistance to organic solvents, periplasmic component, at 3,470,863 to 3,471,321
_3305
Psest_3306: Psest_3306 - conserved hypothetical integral membrane protein, at 3,471,321 to 3,472,118
_3306
Psest_3307: Psest_3307 - ABC-type transport system involved in resistance to organic solvents, ATPase component, at 3,472,111 to 3,472,926
_3307
Position (kb)
3470
3471
3472 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3469.926 kb on + strand at 3470.082 kb on + strand, within Psest_3303 at 3470.082 kb on + strand, within Psest_3303 at 3470.082 kb on + strand, within Psest_3303 at 3470.143 kb on - strand, within Psest_3303 at 3470.148 kb on - strand, within Psest_3303 at 3470.170 kb on + strand, within Psest_3303 at 3470.170 kb on + strand, within Psest_3303 at 3470.170 kb on + strand, within Psest_3303 at 3470.170 kb on + strand, within Psest_3303 at 3470.170 kb on + strand, within Psest_3303 at 3470.170 kb on + strand, within Psest_3303 at 3470.171 kb on - strand, within Psest_3303 at 3470.171 kb on - strand, within Psest_3303 at 3470.171 kb on - strand, within Psest_3303 at 3470.171 kb on - strand, within Psest_3303 at 3470.171 kb on - strand, within Psest_3303 at 3470.171 kb on - strand, within Psest_3303 at 3470.406 kb on + strand, within Psest_3304 at 3470.407 kb on - strand, within Psest_3304 at 3470.407 kb on - strand, within Psest_3304 at 3470.407 kb on - strand, within Psest_3304 at 3470.418 kb on + strand, within Psest_3304 at 3470.496 kb on - strand, within Psest_3304 at 3470.518 kb on - strand, within Psest_3304 at 3470.518 kb on - strand, within Psest_3304 at 3470.538 kb on + strand, within Psest_3304 at 3470.538 kb on + strand, within Psest_3304 at 3470.611 kb on - strand, within Psest_3304 at 3470.611 kb on - strand, within Psest_3304 at 3470.685 kb on + strand, within Psest_3304 at 3470.686 kb on - strand, within Psest_3304 at 3470.686 kb on - strand, within Psest_3304 at 3470.709 kb on + strand, within Psest_3304 at 3470.710 kb on - strand, within Psest_3304 at 3470.710 kb on - strand, within Psest_3304 at 3470.796 kb on + strand at 3470.796 kb on + strand at 3470.796 kb on + strand at 3470.796 kb on + strand at 3470.796 kb on + strand at 3470.796 kb on + strand at 3470.796 kb on + strand at 3470.797 kb on - strand at 3470.797 kb on - strand at 3470.797 kb on - strand at 3470.797 kb on - strand at 3470.797 kb on - strand at 3470.797 kb on - strand at 3470.797 kb on - strand at 3470.858 kb on - strand at 3470.871 kb on + strand at 3470.871 kb on + strand at 3470.872 kb on - strand at 3470.872 kb on - strand at 3470.876 kb on - strand at 3470.911 kb on + strand, within Psest_3305 at 3470.911 kb on + strand, within Psest_3305 at 3470.912 kb on - strand, within Psest_3305 at 3470.912 kb on - strand, within Psest_3305 at 3470.914 kb on + strand, within Psest_3305 at 3470.914 kb on + strand, within Psest_3305 at 3470.993 kb on + strand, within Psest_3305 at 3470.993 kb on + strand, within Psest_3305 at 3470.993 kb on + strand, within Psest_3305 at 3470.993 kb on + strand, within Psest_3305 at 3470.993 kb on + strand, within Psest_3305 at 3470.994 kb on - strand, within Psest_3305 at 3470.994 kb on - strand, within Psest_3305 at 3470.994 kb on - strand, within Psest_3305 at 3470.994 kb on - strand, within Psest_3305 at 3470.994 kb on - strand, within Psest_3305 at 3471.092 kb on + strand, within Psest_3305 at 3471.092 kb on + strand, within Psest_3305 at 3471.092 kb on + strand, within Psest_3305 at 3471.092 kb on + strand, within Psest_3305 at 3471.092 kb on + strand, within Psest_3305 at 3471.092 kb on + strand, within Psest_3305 at 3471.092 kb on + strand, within Psest_3305 at 3471.093 kb on - strand, within Psest_3305 at 3471.093 kb on - strand, within Psest_3305 at 3471.093 kb on - strand, within Psest_3305 at 3471.096 kb on + strand, within Psest_3305 at 3471.096 kb on + strand, within Psest_3305 at 3471.192 kb on - strand, within Psest_3305 at 3471.197 kb on + strand, within Psest_3305 at 3471.197 kb on + strand, within Psest_3305 at 3471.197 kb on + strand, within Psest_3305 at 3471.198 kb on - strand, within Psest_3305 at 3471.206 kb on + strand, within Psest_3305 at 3471.207 kb on - strand, within Psest_3305 at 3471.207 kb on - strand, within Psest_3305 at 3471.207 kb on - strand, within Psest_3305 at 3471.385 kb on + strand at 3471.385 kb on + strand at 3471.385 kb on + strand at 3471.385 kb on + strand at 3471.385 kb on + strand at 3471.385 kb on + strand at 3471.416 kb on + strand, within Psest_3306 at 3471.416 kb on + strand, within Psest_3306 at 3471.565 kb on + strand, within Psest_3306 at 3471.615 kb on + strand, within Psest_3306 at 3471.615 kb on + strand, within Psest_3306 at 3471.682 kb on + strand, within Psest_3306 at 3471.682 kb on + strand, within Psest_3306 at 3471.682 kb on + strand, within Psest_3306 at 3471.682 kb on + strand, within Psest_3306 at 3471.682 kb on + strand, within Psest_3306 at 3471.682 kb on + strand, within Psest_3306 at 3471.682 kb on + strand, within Psest_3306 at 3471.883 kb on + strand, within Psest_3306 at 3472.112 kb on + strand at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.280 kb on + strand, within Psest_3307 at 3472.286 kb on + strand, within Psest_3307 at 3472.286 kb on + strand, within Psest_3307 at 3472.286 kb on + strand, within Psest_3307 at 3472.286 kb on + strand, within Psest_3307 at 3472.286 kb on + strand, within Psest_3307
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 3,469,926 + -0.1 3,470,082 + Psest_3303 0.59 -1.3 3,470,082 + Psest_3303 0.59 -1.4 3,470,082 + Psest_3303 0.59 -1.2 3,470,143 - Psest_3303 0.78 -1.8 3,470,148 - Psest_3303 0.80 -2.8 3,470,170 + Psest_3303 0.87 -1.7 3,470,170 + Psest_3303 0.87 -1.1 3,470,170 + Psest_3303 0.87 -4.1 3,470,170 + Psest_3303 0.87 -1.6 3,470,170 + Psest_3303 0.87 -1.4 3,470,170 + Psest_3303 0.87 -2.1 3,470,171 - Psest_3303 0.87 -1.4 3,470,171 - Psest_3303 0.87 -1.8 3,470,171 - Psest_3303 0.87 -0.4 3,470,171 - Psest_3303 0.87 -0.1 3,470,171 - Psest_3303 0.87 -1.2 3,470,171 - Psest_3303 0.87 -2.7 3,470,406 + Psest_3304 0.31 -0.2 3,470,407 - Psest_3304 0.31 -0.4 3,470,407 - Psest_3304 0.31 -1.6 3,470,407 - Psest_3304 0.31 -3.8 3,470,418 + Psest_3304 0.33 -1.6 3,470,496 - Psest_3304 0.45 -2.1 3,470,518 - Psest_3304 0.48 +1.6 3,470,518 - Psest_3304 0.48 -1.6 3,470,538 + Psest_3304 0.52 -3.9 3,470,538 + Psest_3304 0.52 -1.0 3,470,611 - Psest_3304 0.63 -0.7 3,470,611 - Psest_3304 0.63 -1.4 3,470,685 + Psest_3304 0.74 -1.0 3,470,686 - Psest_3304 0.74 -2.0 3,470,686 - Psest_3304 0.74 +1.6 3,470,709 + Psest_3304 0.78 -0.7 3,470,710 - Psest_3304 0.78 -3.3 3,470,710 - Psest_3304 0.78 -0.7 3,470,796 + -1.6 3,470,796 + -2.1 3,470,796 + -2.9 3,470,796 + -2.1 3,470,796 + -2.4 3,470,796 + -1.8 3,470,796 + -3.1 3,470,797 - -1.2 3,470,797 - -0.7 3,470,797 - +0.0 3,470,797 - -0.6 3,470,797 - -0.4 3,470,797 - -2.4 3,470,797 - -2.3 3,470,858 - -1.9 3,470,871 + -2.8 3,470,871 + -2.4 3,470,872 - -1.8 3,470,872 - -3.5 3,470,876 - -3.1 3,470,911 + Psest_3305 0.10 -2.6 3,470,911 + Psest_3305 0.10 -2.4 3,470,912 - Psest_3305 0.11 -3.0 3,470,912 - Psest_3305 0.11 -2.0 3,470,914 + Psest_3305 0.11 -0.4 3,470,914 + Psest_3305 0.11 -0.7 3,470,993 + Psest_3305 0.28 -3.2 3,470,993 + Psest_3305 0.28 -4.0 3,470,993 + Psest_3305 0.28 -0.4 3,470,993 + Psest_3305 0.28 -2.2 3,470,993 + Psest_3305 0.28 -2.2 3,470,994 - Psest_3305 0.29 -1.7 3,470,994 - Psest_3305 0.29 -1.8 3,470,994 - Psest_3305 0.29 -2.2 3,470,994 - Psest_3305 0.29 -0.7 3,470,994 - Psest_3305 0.29 -1.8 3,471,092 + Psest_3305 0.50 -2.6 3,471,092 + Psest_3305 0.50 -1.6 3,471,092 + Psest_3305 0.50 -0.7 3,471,092 + Psest_3305 0.50 -1.8 3,471,092 + Psest_3305 0.50 -2.6 3,471,092 + Psest_3305 0.50 -1.2 3,471,092 + Psest_3305 0.50 +0.3 3,471,093 - Psest_3305 0.50 -1.0 3,471,093 - Psest_3305 0.50 -2.0 3,471,093 - Psest_3305 0.50 -2.0 3,471,096 + Psest_3305 0.51 -3.4 3,471,096 + Psest_3305 0.51 -1.4 3,471,192 - Psest_3305 0.72 -1.8 3,471,197 + Psest_3305 0.73 -1.6 3,471,197 + Psest_3305 0.73 -1.7 3,471,197 + Psest_3305 0.73 -1.0 3,471,198 - Psest_3305 0.73 -2.3 3,471,206 + Psest_3305 0.75 -1.8 3,471,207 - Psest_3305 0.75 -1.2 3,471,207 - Psest_3305 0.75 -1.2 3,471,207 - Psest_3305 0.75 -1.4 3,471,385 + -1.0 3,471,385 + -0.7 3,471,385 + -0.7 3,471,385 + -1.2 3,471,385 + -0.7 3,471,385 + -0.4 3,471,416 + Psest_3306 0.12 -3.1 3,471,416 + Psest_3306 0.12 -1.6 3,471,565 + Psest_3306 0.31 -1.1 3,471,615 + Psest_3306 0.37 -3.1 3,471,615 + Psest_3306 0.37 -2.7 3,471,682 + Psest_3306 0.45 -2.5 3,471,682 + Psest_3306 0.45 -2.1 3,471,682 + Psest_3306 0.45 -1.2 3,471,682 + Psest_3306 0.45 -1.6 3,471,682 + Psest_3306 0.45 -1.7 3,471,682 + Psest_3306 0.45 -0.8 3,471,682 + Psest_3306 0.45 -1.2 3,471,883 + Psest_3306 0.70 -3.6 3,472,112 + -2.0 3,472,280 + Psest_3307 0.21 -3.4 3,472,280 + Psest_3307 0.21 -1.2 3,472,280 + Psest_3307 0.21 +1.6 3,472,280 + Psest_3307 0.21 -2.5 3,472,280 + Psest_3307 0.21 -1.0 3,472,280 + Psest_3307 0.21 -2.8 3,472,280 + Psest_3307 0.21 -1.8 3,472,280 + Psest_3307 0.21 -3.5 3,472,280 + Psest_3307 0.21 +0.2 3,472,280 + Psest_3307 0.21 -1.4 3,472,280 + Psest_3307 0.21 -2.1 3,472,280 + Psest_3307 0.21 -2.3 3,472,280 + Psest_3307 0.21 -3.0 3,472,280 + Psest_3307 0.21 -2.1 3,472,280 + Psest_3307 0.21 -3.1 3,472,280 + Psest_3307 0.21 -2.0 3,472,280 + Psest_3307 0.21 -0.7 3,472,286 + Psest_3307 0.21 -1.2 3,472,286 + Psest_3307 0.21 -1.6 3,472,286 + Psest_3307 0.21 -1.8 3,472,286 + Psest_3307 0.21 -3.2 3,472,286 + Psest_3307 0.21 -3.9
Or see this region's nucleotide sequence