Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3217 and Psest_3218 are separated by 148 nucleotides Psest_3218 and Psest_3219 are separated by 130 nucleotides Psest_3219 and Psest_3221 are separated by 364 nucleotides
Psest_3217: Psest_3217 - PAS domain S-box, at 3,387,688 to 3,391,407
_3217
Psest_3218: Psest_3218 - Outer membrane protein and related peptidoglycan-associated (lipo)proteins, at 3,391,556 to 3,392,209
_3218
Psest_3219: Psest_3219 - Zn-dependent hydrolases, including glyoxylases, at 3,392,340 to 3,392,990
_3219
Psest_3221: Psest_3221 - Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II, at 3,393,355 to 3,395,040
_3221
Position (kb)
3392
3393 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3391.360 kb on + strand at 3391.360 kb on + strand at 3391.459 kb on - strand at 3391.542 kb on + strand at 3391.547 kb on + strand at 3391.547 kb on + strand at 3391.548 kb on - strand at 3391.550 kb on + strand at 3391.550 kb on + strand at 3391.557 kb on + strand at 3391.558 kb on - strand at 3391.650 kb on + strand, within Psest_3218 at 3391.692 kb on + strand, within Psest_3218 at 3391.753 kb on + strand, within Psest_3218 at 3391.763 kb on - strand, within Psest_3218 at 3391.873 kb on + strand, within Psest_3218 at 3391.873 kb on + strand, within Psest_3218 at 3391.874 kb on - strand, within Psest_3218 at 3391.874 kb on - strand, within Psest_3218 at 3391.874 kb on - strand, within Psest_3218 at 3391.874 kb on - strand, within Psest_3218 at 3391.977 kb on + strand, within Psest_3218 at 3391.977 kb on + strand, within Psest_3218 at 3391.977 kb on + strand, within Psest_3218 at 3391.977 kb on + strand, within Psest_3218 at 3391.977 kb on + strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.980 kb on + strand, within Psest_3218 at 3392.104 kb on - strand, within Psest_3218 at 3392.104 kb on - strand, within Psest_3218 at 3392.104 kb on - strand, within Psest_3218 at 3392.105 kb on + strand, within Psest_3218 at 3392.105 kb on + strand, within Psest_3218 at 3392.105 kb on + strand, within Psest_3218 at 3392.105 kb on + strand, within Psest_3218 at 3392.105 kb on + strand, within Psest_3218 at 3392.105 kb on + strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.139 kb on + strand, within Psest_3218 at 3392.140 kb on - strand, within Psest_3218 at 3392.140 kb on - strand, within Psest_3218 at 3392.153 kb on - strand at 3392.209 kb on + strand at 3392.210 kb on - strand at 3392.213 kb on + strand at 3392.213 kb on + strand at 3392.213 kb on + strand at 3392.213 kb on + strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.233 kb on + strand at 3392.254 kb on - strand at 3392.279 kb on - strand at 3392.279 kb on - strand at 3392.279 kb on - strand at 3392.283 kb on + strand at 3392.283 kb on + strand at 3392.283 kb on + strand at 3392.283 kb on + strand at 3392.284 kb on - strand at 3392.305 kb on + strand at 3392.305 kb on + strand at 3392.306 kb on - strand at 3392.306 kb on - strand at 3392.438 kb on + strand, within Psest_3219 at 3392.438 kb on + strand, within Psest_3219 at 3392.438 kb on + strand, within Psest_3219 at 3392.439 kb on - strand, within Psest_3219 at 3392.439 kb on - strand, within Psest_3219 at 3392.440 kb on + strand, within Psest_3219 at 3392.440 kb on + strand, within Psest_3219 at 3392.441 kb on - strand, within Psest_3219 at 3392.441 kb on - strand, within Psest_3219 at 3392.441 kb on - strand, within Psest_3219 at 3392.582 kb on + strand, within Psest_3219 at 3392.582 kb on + strand, within Psest_3219 at 3392.583 kb on - strand, within Psest_3219 at 3392.583 kb on - strand, within Psest_3219 at 3392.583 kb on - strand, within Psest_3219 at 3392.583 kb on - strand, within Psest_3219 at 3392.671 kb on + strand, within Psest_3219 at 3392.672 kb on - strand, within Psest_3219 at 3392.672 kb on - strand, within Psest_3219 at 3392.672 kb on - strand, within Psest_3219 at 3393.012 kb on + strand at 3393.319 kb on + strand at 3393.319 kb on + strand at 3393.320 kb on - strand at 3393.328 kb on + strand at 3393.501 kb on + strand at 3393.501 kb on + strand at 3393.501 kb on + strand at 3393.502 kb on - strand at 3393.502 kb on - strand at 3393.631 kb on + strand, within Psest_3221 at 3393.631 kb on + strand, within Psest_3221 at 3393.824 kb on + strand, within Psest_3221 at 3393.824 kb on + strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.827 kb on + strand, within Psest_3221 at 3393.827 kb on + strand, within Psest_3221 at 3393.828 kb on - strand, within Psest_3221 at 3393.828 kb on - strand, within Psest_3221 at 3393.838 kb on - strand, within Psest_3221 at 3393.857 kb on - strand, within Psest_3221
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 3,391,360 + +0.5 3,391,360 + +0.6 3,391,459 - -1.3 3,391,542 + -1.4 3,391,547 + -0.6 3,391,547 + -2.3 3,391,548 - -2.2 3,391,550 + -0.3 3,391,550 + -2.0 3,391,557 + +0.1 3,391,558 - +0.9 3,391,650 + Psest_3218 0.14 -0.4 3,391,692 + Psest_3218 0.21 +1.3 3,391,753 + Psest_3218 0.30 +0.2 3,391,763 - Psest_3218 0.32 -1.5 3,391,873 + Psest_3218 0.48 -1.6 3,391,873 + Psest_3218 0.48 -0.3 3,391,874 - Psest_3218 0.49 -1.5 3,391,874 - Psest_3218 0.49 -1.2 3,391,874 - Psest_3218 0.49 -1.2 3,391,874 - Psest_3218 0.49 -0.9 3,391,977 + Psest_3218 0.64 -2.4 3,391,977 + Psest_3218 0.64 -2.3 3,391,977 + Psest_3218 0.64 -1.6 3,391,977 + Psest_3218 0.64 +0.6 3,391,977 + Psest_3218 0.64 -1.3 3,391,978 - Psest_3218 0.65 -0.4 3,391,978 - Psest_3218 0.65 +0.3 3,391,978 - Psest_3218 0.65 -2.0 3,391,978 - Psest_3218 0.65 -1.0 3,391,978 - Psest_3218 0.65 +1.2 3,391,978 - Psest_3218 0.65 -0.9 3,391,978 - Psest_3218 0.65 -2.0 3,391,980 + Psest_3218 0.65 -1.4 3,392,104 - Psest_3218 0.84 -3.3 3,392,104 - Psest_3218 0.84 +0.2 3,392,104 - Psest_3218 0.84 -0.8 3,392,105 + Psest_3218 0.84 -1.1 3,392,105 + Psest_3218 0.84 -1.6 3,392,105 + Psest_3218 0.84 -1.4 3,392,105 + Psest_3218 0.84 -1.2 3,392,105 + Psest_3218 0.84 -1.1 3,392,105 + Psest_3218 0.84 +0.6 3,392,106 - Psest_3218 0.84 -3.3 3,392,106 - Psest_3218 0.84 -0.3 3,392,106 - Psest_3218 0.84 -3.1 3,392,106 - Psest_3218 0.84 -1.5 3,392,106 - Psest_3218 0.84 -2.9 3,392,106 - Psest_3218 0.84 -0.4 3,392,106 - Psest_3218 0.84 -0.9 3,392,106 - Psest_3218 0.84 -2.2 3,392,106 - Psest_3218 0.84 -1.8 3,392,106 - Psest_3218 0.84 -0.5 3,392,106 - Psest_3218 0.84 -1.4 3,392,106 - Psest_3218 0.84 -1.6 3,392,106 - Psest_3218 0.84 -0.4 3,392,106 - Psest_3218 0.84 +1.6 3,392,106 - Psest_3218 0.84 -1.9 3,392,106 - Psest_3218 0.84 -0.3 3,392,106 - Psest_3218 0.84 -1.0 3,392,106 - Psest_3218 0.84 -2.1 3,392,139 + Psest_3218 0.89 -0.2 3,392,140 - Psest_3218 0.89 -1.4 3,392,140 - Psest_3218 0.89 -2.6 3,392,153 - -1.7 3,392,209 + -1.8 3,392,210 - -3.5 3,392,213 + +0.5 3,392,213 + -2.3 3,392,213 + -0.3 3,392,213 + +0.2 3,392,214 - +0.5 3,392,214 - -1.4 3,392,214 - +0.1 3,392,214 - +0.8 3,392,214 - +0.5 3,392,214 - -1.6 3,392,214 - -1.5 3,392,214 - -3.2 3,392,214 - -1.7 3,392,214 - +0.6 3,392,214 - -2.3 3,392,214 - -1.4 3,392,233 + -2.9 3,392,254 - +0.0 3,392,279 - -1.2 3,392,279 - +1.3 3,392,279 - +0.0 3,392,283 + -2.4 3,392,283 + -0.1 3,392,283 + -1.0 3,392,283 + -0.6 3,392,284 - +0.5 3,392,305 + -0.1 3,392,305 + +1.3 3,392,306 - -0.2 3,392,306 - -0.6 3,392,438 + Psest_3219 0.15 -3.4 3,392,438 + Psest_3219 0.15 -0.8 3,392,438 + Psest_3219 0.15 -1.1 3,392,439 - Psest_3219 0.15 +0.4 3,392,439 - Psest_3219 0.15 +0.7 3,392,440 + Psest_3219 0.15 -0.1 3,392,440 + Psest_3219 0.15 -1.9 3,392,441 - Psest_3219 0.16 +0.2 3,392,441 - Psest_3219 0.16 -0.2 3,392,441 - Psest_3219 0.16 -1.0 3,392,582 + Psest_3219 0.37 +0.3 3,392,582 + Psest_3219 0.37 -1.0 3,392,583 - Psest_3219 0.37 +0.6 3,392,583 - Psest_3219 0.37 +0.3 3,392,583 - Psest_3219 0.37 -0.3 3,392,583 - Psest_3219 0.37 +1.2 3,392,671 + Psest_3219 0.51 -0.1 3,392,672 - Psest_3219 0.51 +0.1 3,392,672 - Psest_3219 0.51 +0.3 3,392,672 - Psest_3219 0.51 +1.9 3,393,012 + -1.8 3,393,319 + +1.7 3,393,319 + -1.2 3,393,320 - +0.4 3,393,328 + -0.4 3,393,501 + -0.4 3,393,501 + -1.1 3,393,501 + -2.1 3,393,502 - +0.7 3,393,502 - -0.2 3,393,631 + Psest_3221 0.16 -1.2 3,393,631 + Psest_3221 0.16 -0.3 3,393,824 + Psest_3221 0.28 -1.5 3,393,824 + Psest_3221 0.28 -0.4 3,393,825 - Psest_3221 0.28 -1.0 3,393,825 - Psest_3221 0.28 -0.3 3,393,825 - Psest_3221 0.28 -2.4 3,393,825 - Psest_3221 0.28 +0.8 3,393,825 - Psest_3221 0.28 +0.6 3,393,825 - Psest_3221 0.28 +1.6 3,393,825 - Psest_3221 0.28 +0.5 3,393,827 + Psest_3221 0.28 -0.4 3,393,827 + Psest_3221 0.28 -0.1 3,393,828 - Psest_3221 0.28 -1.6 3,393,828 - Psest_3221 0.28 +0.9 3,393,838 - Psest_3221 0.29 +0.4 3,393,857 - Psest_3221 0.30 -0.6
Or see this region's nucleotide sequence