Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2129 and Psest_2130 are separated by 94 nucleotides Psest_2130 and Psest_2131 are separated by 202 nucleotides Psest_2131 and Psest_2132 are separated by 63 nucleotides
Psest_2129: Psest_2129 - ABC-type phosphate transport system, periplasmic component, at 2,261,066 to 2,262,424
_2129
Psest_2130: Psest_2130 - exodeoxyribonuclease III, at 2,262,519 to 2,263,331
_2130
Psest_2131: Psest_2131 - Acetyltransferases, at 2,263,534 to 2,264,169
_2131
Psest_2132: Psest_2132 - Outer membrane protein, at 2,264,233 to 2,266,047
_2132
Position (kb)
2262
2263
2264 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2261.535 kb on + strand, within Psest_2129 at 2261.535 kb on + strand, within Psest_2129 at 2261.535 kb on + strand, within Psest_2129 at 2261.535 kb on + strand, within Psest_2129 at 2261.536 kb on - strand, within Psest_2129 at 2261.536 kb on - strand, within Psest_2129 at 2261.536 kb on - strand, within Psest_2129 at 2261.536 kb on - strand, within Psest_2129 at 2261.536 kb on - strand, within Psest_2129 at 2261.574 kb on + strand, within Psest_2129 at 2261.574 kb on + strand, within Psest_2129 at 2261.574 kb on + strand, within Psest_2129 at 2261.574 kb on + strand, within Psest_2129 at 2261.826 kb on + strand, within Psest_2129 at 2261.826 kb on + strand, within Psest_2129 at 2261.826 kb on + strand, within Psest_2129 at 2261.826 kb on + strand, within Psest_2129 at 2261.826 kb on + strand, within Psest_2129 at 2261.827 kb on - strand, within Psest_2129 at 2261.827 kb on - strand, within Psest_2129 at 2261.827 kb on - strand, within Psest_2129 at 2261.827 kb on - strand, within Psest_2129 at 2261.827 kb on - strand, within Psest_2129 at 2261.827 kb on - strand, within Psest_2129 at 2261.827 kb on - strand, within Psest_2129 at 2261.827 kb on - strand, within Psest_2129 at 2261.827 kb on - strand, within Psest_2129 at 2261.827 kb on - strand, within Psest_2129 at 2262.150 kb on + strand, within Psest_2129 at 2262.151 kb on - strand, within Psest_2129 at 2262.440 kb on + strand at 2262.440 kb on + strand at 2262.440 kb on + strand at 2262.441 kb on - strand at 2262.441 kb on - strand at 2262.441 kb on - strand at 2262.441 kb on - strand at 2262.457 kb on - strand at 2262.501 kb on + strand at 2262.502 kb on - strand at 2262.634 kb on + strand, within Psest_2130 at 2262.635 kb on - strand, within Psest_2130 at 2262.635 kb on - strand, within Psest_2130 at 2262.943 kb on + strand, within Psest_2130 at 2262.944 kb on - strand, within Psest_2130 at 2263.145 kb on - strand, within Psest_2130 at 2263.165 kb on + strand, within Psest_2130 at 2263.350 kb on + strand at 2263.445 kb on - strand at 2263.445 kb on - strand at 2263.445 kb on - strand at 2263.455 kb on - strand at 2263.492 kb on + strand at 2263.493 kb on - strand at 2263.601 kb on - strand, within Psest_2131 at 2263.601 kb on - strand, within Psest_2131 at 2263.633 kb on + strand, within Psest_2131 at 2263.713 kb on - strand, within Psest_2131 at 2263.937 kb on + strand, within Psest_2131 at 2263.937 kb on + strand, within Psest_2131 at 2263.937 kb on + strand, within Psest_2131 at 2263.937 kb on + strand, within Psest_2131 at 2263.938 kb on - strand, within Psest_2131 at 2263.938 kb on - strand, within Psest_2131 at 2264.065 kb on + strand, within Psest_2131 at 2264.168 kb on - strand at 2264.260 kb on - strand at 2264.270 kb on + strand at 2264.306 kb on + strand at 2264.307 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,261,535 + Psest_2129 0.35 +0.1 2,261,535 + Psest_2129 0.35 -1.6 2,261,535 + Psest_2129 0.35 -2.0 2,261,535 + Psest_2129 0.35 +0.2 2,261,536 - Psest_2129 0.35 +0.6 2,261,536 - Psest_2129 0.35 +1.0 2,261,536 - Psest_2129 0.35 -0.7 2,261,536 - Psest_2129 0.35 -3.0 2,261,536 - Psest_2129 0.35 -0.2 2,261,574 + Psest_2129 0.37 -1.5 2,261,574 + Psest_2129 0.37 -0.7 2,261,574 + Psest_2129 0.37 -1.1 2,261,574 + Psest_2129 0.37 +0.8 2,261,826 + Psest_2129 0.56 +0.6 2,261,826 + Psest_2129 0.56 -2.2 2,261,826 + Psest_2129 0.56 +2.5 2,261,826 + Psest_2129 0.56 -0.8 2,261,826 + Psest_2129 0.56 -0.1 2,261,827 - Psest_2129 0.56 +0.8 2,261,827 - Psest_2129 0.56 +0.3 2,261,827 - Psest_2129 0.56 -1.1 2,261,827 - Psest_2129 0.56 +1.5 2,261,827 - Psest_2129 0.56 -2.7 2,261,827 - Psest_2129 0.56 +1.5 2,261,827 - Psest_2129 0.56 +0.5 2,261,827 - Psest_2129 0.56 -0.2 2,261,827 - Psest_2129 0.56 +1.0 2,261,827 - Psest_2129 0.56 -0.1 2,262,150 + Psest_2129 0.80 -0.5 2,262,151 - Psest_2129 0.80 +0.7 2,262,440 + +2.0 2,262,440 + +0.3 2,262,440 + -1.0 2,262,441 - +1.1 2,262,441 - -1.8 2,262,441 - +0.3 2,262,441 - +0.8 2,262,457 - -2.6 2,262,501 + +0.2 2,262,502 - +1.2 2,262,634 + Psest_2130 0.14 -0.3 2,262,635 - Psest_2130 0.14 -0.5 2,262,635 - Psest_2130 0.14 -2.5 2,262,943 + Psest_2130 0.52 -1.9 2,262,944 - Psest_2130 0.52 +2.1 2,263,145 - Psest_2130 0.77 +2.4 2,263,165 + Psest_2130 0.79 +1.3 2,263,350 + +1.1 2,263,445 - +1.3 2,263,445 - +0.2 2,263,445 - -0.3 2,263,455 - -1.5 2,263,492 + -0.9 2,263,493 - +0.8 2,263,601 - Psest_2131 0.11 -0.1 2,263,601 - Psest_2131 0.11 -1.8 2,263,633 + Psest_2131 0.16 +0.5 2,263,713 - Psest_2131 0.28 -0.3 2,263,937 + Psest_2131 0.63 +0.6 2,263,937 + Psest_2131 0.63 -0.7 2,263,937 + Psest_2131 0.63 +0.9 2,263,937 + Psest_2131 0.63 -0.7 2,263,938 - Psest_2131 0.64 +2.3 2,263,938 - Psest_2131 0.64 +1.2 2,264,065 + Psest_2131 0.83 -1.0 2,264,168 - -1.6 2,264,260 - -1.3 2,264,270 + +0.5 2,264,306 + +0.7 2,264,307 - -1.0
Or see this region's nucleotide sequence