Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1727 and Psest_1728 are separated by 242 nucleotides Psest_1728 and Psest_1729 are separated by 12 nucleotides Psest_1729 and Psest_1730 are separated by 40 nucleotides Psest_1730 and Psest_1731 are separated by 1 nucleotides Psest_1731 and Psest_1732 overlap by 1 nucleotides
Psest_1727: Psest_1727 - Flagellar hook-length control protein, at 1,833,221 to 1,834,462
_1727
Psest_1728: Psest_1728 - Flagellar basal body-associated protein, at 1,834,705 to 1,835,238
_1728
Psest_1729: Psest_1729 - flagellar motor switch protein FliM, at 1,835,251 to 1,836,222
_1729
Psest_1730: Psest_1730 - flagellar motor switch protein FliN, at 1,836,263 to 1,836,736
_1730
Psest_1731: Psest_1731 - flagellar biosynthetic protein FliO, at 1,836,738 to 1,837,157
_1731
Psest_1732: Psest_1732 - flagellar biosynthetic protein FliP, at 1,837,157 to 1,837,933
_1732
Position (kb)
1835
1836
1837 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1834.659 kb on - strand at 1834.665 kb on + strand at 1834.666 kb on - strand at 1834.677 kb on + strand at 1834.677 kb on + strand at 1834.678 kb on - strand at 1834.678 kb on - strand at 1834.811 kb on + strand, within Psest_1728 at 1834.926 kb on + strand, within Psest_1728 at 1834.927 kb on - strand, within Psest_1728 at 1834.927 kb on - strand, within Psest_1728 at 1834.930 kb on + strand, within Psest_1728 at 1834.931 kb on - strand, within Psest_1728 at 1834.931 kb on - strand, within Psest_1728 at 1834.931 kb on - strand, within Psest_1728 at 1834.988 kb on - strand, within Psest_1728 at 1834.988 kb on - strand, within Psest_1728 at 1834.988 kb on - strand, within Psest_1728 at 1834.988 kb on - strand, within Psest_1728 at 1835.052 kb on + strand, within Psest_1728 at 1835.052 kb on + strand, within Psest_1728 at 1835.053 kb on - strand, within Psest_1728 at 1835.053 kb on - strand, within Psest_1728 at 1835.236 kb on + strand at 1835.236 kb on + strand at 1835.237 kb on - strand at 1835.237 kb on - strand at 1835.524 kb on + strand, within Psest_1729 at 1835.525 kb on - strand, within Psest_1729 at 1835.539 kb on + strand, within Psest_1729 at 1835.539 kb on + strand, within Psest_1729 at 1835.539 kb on + strand, within Psest_1729 at 1835.540 kb on - strand, within Psest_1729 at 1835.540 kb on - strand, within Psest_1729 at 1835.540 kb on - strand, within Psest_1729 at 1835.782 kb on + strand, within Psest_1729 at 1836.233 kb on + strand at 1836.234 kb on - strand at 1836.815 kb on + strand, within Psest_1731 at 1837.174 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,834,659 - +2.7 1,834,665 + -0.6 1,834,666 - +4.0 1,834,677 + -0.2 1,834,677 + -0.5 1,834,678 - +4.6 1,834,678 - +4.0 1,834,811 + Psest_1728 0.20 -0.3 1,834,926 + Psest_1728 0.41 +0.0 1,834,927 - Psest_1728 0.42 +4.9 1,834,927 - Psest_1728 0.42 +3.8 1,834,930 + Psest_1728 0.42 -0.6 1,834,931 - Psest_1728 0.42 +3.3 1,834,931 - Psest_1728 0.42 +1.4 1,834,931 - Psest_1728 0.42 +4.8 1,834,988 - Psest_1728 0.53 +2.5 1,834,988 - Psest_1728 0.53 -2.5 1,834,988 - Psest_1728 0.53 +1.8 1,834,988 - Psest_1728 0.53 +3.2 1,835,052 + Psest_1728 0.65 -0.2 1,835,052 + Psest_1728 0.65 -0.3 1,835,053 - Psest_1728 0.65 +1.6 1,835,053 - Psest_1728 0.65 +2.8 1,835,236 + +1.6 1,835,236 + +0.8 1,835,237 - +3.8 1,835,237 - +3.7 1,835,524 + Psest_1729 0.28 +0.7 1,835,525 - Psest_1729 0.28 +3.5 1,835,539 + Psest_1729 0.30 +1.4 1,835,539 + Psest_1729 0.30 +2.2 1,835,539 + Psest_1729 0.30 +2.3 1,835,540 - Psest_1729 0.30 +3.3 1,835,540 - Psest_1729 0.30 +3.8 1,835,540 - Psest_1729 0.30 +2.5 1,835,782 + Psest_1729 0.55 +2.6 1,836,233 + -1.7 1,836,234 - +2.1 1,836,815 + Psest_1731 0.18 +2.8 1,837,174 - +4.9
Or see this region's nucleotide sequence