Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1621 and Psest_1622 are separated by 9 nucleotides Psest_1622 and Psest_1623 are separated by 94 nucleotides Psest_1623 and Psest_1624 are separated by 28 nucleotides
Psest_1621: Psest_1621 - Uncharacterized protein conserved in bacteria, at 1,725,084 to 1,726,214
_1621
Psest_1622: Psest_1622 - hypothetical protein, at 1,726,224 to 1,726,646
_1622
Psest_1623: Psest_1623 - Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family, at 1,726,741 to 1,727,295
_1623
Psest_1624: Psest_1624 - Transcriptional regulator, at 1,727,324 to 1,728,232
_1624
Position (kb)
1726
1727 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1725.306 kb on + strand, within Psest_1621 at 1725.307 kb on - strand, within Psest_1621 at 1725.315 kb on + strand, within Psest_1621 at 1725.315 kb on + strand, within Psest_1621 at 1725.315 kb on + strand, within Psest_1621 at 1725.315 kb on + strand, within Psest_1621 at 1725.316 kb on - strand, within Psest_1621 at 1725.316 kb on - strand, within Psest_1621 at 1725.316 kb on - strand, within Psest_1621 at 1725.316 kb on - strand, within Psest_1621 at 1725.369 kb on + strand, within Psest_1621 at 1725.656 kb on - strand, within Psest_1621 at 1725.747 kb on + strand, within Psest_1621 at 1725.769 kb on - strand, within Psest_1621 at 1725.769 kb on - strand, within Psest_1621 at 1725.769 kb on - strand, within Psest_1621 at 1725.769 kb on - strand, within Psest_1621 at 1725.918 kb on + strand, within Psest_1621 at 1726.092 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.097 kb on - strand, within Psest_1621 at 1726.097 kb on - strand, within Psest_1621 at 1726.097 kb on - strand, within Psest_1621 at 1726.212 kb on + strand at 1726.212 kb on + strand at 1726.213 kb on - strand at 1726.316 kb on - strand, within Psest_1622 at 1726.389 kb on - strand, within Psest_1622 at 1726.406 kb on - strand, within Psest_1622 at 1726.406 kb on - strand, within Psest_1622 at 1726.406 kb on - strand, within Psest_1622 at 1726.584 kb on + strand, within Psest_1622 at 1726.584 kb on + strand, within Psest_1622 at 1726.585 kb on - strand, within Psest_1622 at 1726.694 kb on - strand at 1726.705 kb on + strand at 1726.784 kb on - strand at 1726.852 kb on + strand, within Psest_1623 at 1726.853 kb on - strand, within Psest_1623 at 1727.117 kb on + strand, within Psest_1623 at 1727.131 kb on + strand, within Psest_1623 at 1727.131 kb on + strand, within Psest_1623 at 1727.131 kb on + strand, within Psest_1623 at 1727.132 kb on - strand, within Psest_1623 at 1727.136 kb on - strand, within Psest_1623 at 1727.338 kb on + strand at 1727.338 kb on + strand at 1727.339 kb on - strand at 1727.358 kb on + strand at 1727.358 kb on + strand at 1727.359 kb on - strand at 1727.416 kb on + strand, within Psest_1624 at 1727.421 kb on + strand, within Psest_1624 at 1727.421 kb on + strand, within Psest_1624 at 1727.422 kb on - strand, within Psest_1624 at 1727.422 kb on - strand, within Psest_1624 at 1727.422 kb on - strand, within Psest_1624 at 1727.427 kb on + strand, within Psest_1624 at 1727.427 kb on + strand, within Psest_1624 at 1727.427 kb on + strand, within Psest_1624 at 1727.427 kb on + strand, within Psest_1624 at 1727.427 kb on + strand, within Psest_1624 at 1727.427 kb on + strand, within Psest_1624 at 1727.428 kb on - strand, within Psest_1624 at 1727.428 kb on - strand, within Psest_1624 at 1727.428 kb on - strand, within Psest_1624
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,725,306 + Psest_1621 0.20 -1.6 1,725,307 - Psest_1621 0.20 +0.7 1,725,315 + Psest_1621 0.20 +1.5 1,725,315 + Psest_1621 0.20 +0.0 1,725,315 + Psest_1621 0.20 +2.7 1,725,315 + Psest_1621 0.20 +0.5 1,725,316 - Psest_1621 0.21 -0.7 1,725,316 - Psest_1621 0.21 -1.1 1,725,316 - Psest_1621 0.21 -0.8 1,725,316 - Psest_1621 0.21 +1.4 1,725,369 + Psest_1621 0.25 -1.6 1,725,656 - Psest_1621 0.51 +0.7 1,725,747 + Psest_1621 0.59 -1.9 1,725,769 - Psest_1621 0.61 +2.4 1,725,769 - Psest_1621 0.61 -1.0 1,725,769 - Psest_1621 0.61 +0.5 1,725,769 - Psest_1621 0.61 -0.8 1,725,918 + Psest_1621 0.74 -1.9 1,726,092 + Psest_1621 0.89 +0.0 1,726,096 + Psest_1621 0.89 -0.3 1,726,096 + Psest_1621 0.89 -0.9 1,726,096 + Psest_1621 0.89 -3.1 1,726,096 + Psest_1621 0.89 +0.2 1,726,096 + Psest_1621 0.89 -1.6 1,726,096 + Psest_1621 0.89 -0.3 1,726,096 + Psest_1621 0.89 +0.6 1,726,096 + Psest_1621 0.89 +1.8 1,726,097 - Psest_1621 0.90 +1.9 1,726,097 - Psest_1621 0.90 -0.8 1,726,097 - Psest_1621 0.90 +0.0 1,726,212 + +0.9 1,726,212 + -0.4 1,726,213 - +0.1 1,726,316 - Psest_1622 0.22 -0.5 1,726,389 - Psest_1622 0.39 -0.9 1,726,406 - Psest_1622 0.43 -1.0 1,726,406 - Psest_1622 0.43 -0.6 1,726,406 - Psest_1622 0.43 -1.9 1,726,584 + Psest_1622 0.85 -0.9 1,726,584 + Psest_1622 0.85 -1.7 1,726,585 - Psest_1622 0.85 -3.8 1,726,694 - +1.0 1,726,705 + +0.1 1,726,784 - -0.2 1,726,852 + Psest_1623 0.20 -2.9 1,726,853 - Psest_1623 0.20 +1.2 1,727,117 + Psest_1623 0.68 +0.1 1,727,131 + Psest_1623 0.70 +0.6 1,727,131 + Psest_1623 0.70 -0.4 1,727,131 + Psest_1623 0.70 +2.1 1,727,132 - Psest_1623 0.70 -0.3 1,727,136 - Psest_1623 0.71 +1.1 1,727,338 + +0.1 1,727,338 + +0.4 1,727,339 - +1.9 1,727,358 + -0.7 1,727,358 + +0.6 1,727,359 - +2.0 1,727,416 + Psest_1624 0.10 +1.5 1,727,421 + Psest_1624 0.11 +0.9 1,727,421 + Psest_1624 0.11 -1.2 1,727,422 - Psest_1624 0.11 +0.8 1,727,422 - Psest_1624 0.11 -0.7 1,727,422 - Psest_1624 0.11 +2.0 1,727,427 + Psest_1624 0.11 +1.0 1,727,427 + Psest_1624 0.11 +0.2 1,727,427 + Psest_1624 0.11 -0.6 1,727,427 + Psest_1624 0.11 -2.7 1,727,427 + Psest_1624 0.11 -0.3 1,727,427 + Psest_1624 0.11 -1.2 1,727,428 - Psest_1624 0.11 -1.8 1,727,428 - Psest_1624 0.11 -1.2 1,727,428 - Psest_1624 0.11 -0.8
Or see this region's nucleotide sequence