Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1242 and Psest_1243 are separated by 42 nucleotides Psest_1243 and Psest_1244 are separated by 64 nucleotides Psest_1244 and Psest_1245 are separated by 12 nucleotides
Psest_1242: Psest_1242 - tRNA-guanine transglycosylase, queuosine-34-forming, at 1,350,083 to 1,351,216
_1242
Psest_1243: Psest_1243 - preprotein translocase, YajC subunit, at 1,351,259 to 1,351,588
_1243
Psest_1244: Psest_1244 - protein-export membrane protein, SecD/SecF family, at 1,351,653 to 1,353,521
_1244
Psest_1245: Psest_1245 - protein-export membrane protein, SecD/SecF family, at 1,353,534 to 1,354,445
_1245
Position (kb)
1351
1352
1353
1354 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1350.661 kb on + strand, within Psest_1242 at 1351.089 kb on + strand, within Psest_1242 at 1351.089 kb on + strand, within Psest_1242 at 1351.089 kb on + strand, within Psest_1242 at 1351.093 kb on + strand, within Psest_1242 at 1351.150 kb on + strand at 1351.150 kb on + strand at 1351.178 kb on + strand at 1351.638 kb on + strand at 1353.519 kb on + strand at 1354.417 kb on + strand at 1354.417 kb on + strand at 1354.417 kb on + strand at 1354.417 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 1,350,661 + Psest_1242 0.51 -0.5 1,351,089 + Psest_1242 0.89 +0.5 1,351,089 + Psest_1242 0.89 +0.5 1,351,089 + Psest_1242 0.89 +0.3 1,351,093 + Psest_1242 0.89 -0.6 1,351,150 + +1.3 1,351,150 + -0.8 1,351,178 + -0.3 1,351,638 + -1.0 1,353,519 + +1.0 1,354,417 + -0.8 1,354,417 + +4.3 1,354,417 + +1.1 1,354,417 + +0.1
Or see this region's nucleotide sequence