Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0483 and Psest_0484 overlap by 4 nucleotides Psest_0484 and Psest_0485 overlap by 4 nucleotides Psest_0485 and Psest_0486 overlap by 4 nucleotides
Psest_0483: Psest_0483 - DNA topoisomerase IV, B subunit, proteobacterial, at 532,502 to 534,481
_0483
Psest_0484: Psest_0484 - hypothetical protein, at 534,478 to 535,461
_0484
Psest_0485: Psest_0485 - clan AA aspartic protease, TIGR02281 family, at 535,458 to 535,985
_0485
Psest_0486: Psest_0486 - DNA topoisomerase IV, A subunit, proteobacterial, at 535,982 to 538,234
_0486
Position (kb) 534
535
536 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 534.675 kb on + strand, within Psest_0484 at 534.759 kb on + strand, within Psest_0484 at 534.759 kb on + strand, within Psest_0484 at 534.841 kb on + strand, within Psest_0484 at 535.094 kb on + strand, within Psest_0484 at 535.095 kb on - strand, within Psest_0484 at 535.315 kb on + strand, within Psest_0484 at 535.371 kb on + strand at 535.371 kb on + strand at 535.371 kb on + strand at 535.371 kb on + strand at 535.371 kb on + strand at 535.612 kb on + strand, within Psest_0485 at 535.618 kb on + strand, within Psest_0485 at 535.618 kb on + strand, within Psest_0485 at 535.619 kb on - strand, within Psest_0485 at 535.619 kb on - strand, within Psest_0485 at 535.619 kb on - strand, within Psest_0485 at 535.619 kb on - strand, within Psest_0485 at 535.664 kb on + strand, within Psest_0485 at 535.665 kb on - strand, within Psest_0485
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 534,675 + Psest_0484 0.20 +1.8 534,759 + Psest_0484 0.29 +2.2 534,759 + Psest_0484 0.29 +1.8 534,841 + Psest_0484 0.37 +0.7 535,094 + Psest_0484 0.63 -0.1 535,095 - Psest_0484 0.63 +1.5 535,315 + Psest_0484 0.85 +1.7 535,371 + +3.7 535,371 + +5.3 535,371 + +3.3 535,371 + +5.3 535,371 + +0.2 535,612 + Psest_0485 0.29 +1.5 535,618 + Psest_0485 0.30 -2.5 535,618 + Psest_0485 0.30 +0.4 535,619 - Psest_0485 0.30 -0.5 535,619 - Psest_0485 0.30 -2.3 535,619 - Psest_0485 0.30 -1.5 535,619 - Psest_0485 0.30 -2.8 535,664 + Psest_0485 0.39 +1.5 535,665 - Psest_0485 0.39 -1.1
Or see this region's nucleotide sequence