Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0422 and Psest_0423 overlap by 8 nucleotides Psest_0423 and Psest_0424 overlap by 8 nucleotides
Psest_0422: Psest_0422 - Site-specific recombinase XerD, at 457,937 to 459,058
_0422
Psest_0423: Psest_0423 - hypothetical protein, at 459,051 to 461,111
_0423
Psest_0424: Psest_0424 - Phage integrase family., at 461,104 to 462,975
_0424
Position (kb) 459
460
461
462 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 458.116 kb on + strand, within Psest_0422 at 458.116 kb on + strand, within Psest_0422 at 458.164 kb on - strand, within Psest_0422 at 458.192 kb on + strand, within Psest_0422 at 458.196 kb on + strand, within Psest_0422 at 458.253 kb on + strand, within Psest_0422 at 458.254 kb on - strand, within Psest_0422 at 458.310 kb on + strand, within Psest_0422 at 458.490 kb on + strand, within Psest_0422 at 458.503 kb on + strand, within Psest_0422 at 458.798 kb on - strand, within Psest_0422 at 458.798 kb on - strand, within Psest_0422 at 458.872 kb on + strand, within Psest_0422 at 458.872 kb on + strand, within Psest_0422 at 458.876 kb on + strand, within Psest_0422 at 458.891 kb on + strand, within Psest_0422 at 459.005 kb on + strand at 459.006 kb on - strand at 459.007 kb on + strand at 459.007 kb on + strand at 459.008 kb on - strand at 459.157 kb on - strand at 459.232 kb on - strand at 459.232 kb on - strand at 459.245 kb on + strand at 459.462 kb on + strand, within Psest_0423 at 459.630 kb on - strand, within Psest_0423 at 459.643 kb on + strand, within Psest_0423 at 459.644 kb on - strand, within Psest_0423 at 459.716 kb on + strand, within Psest_0423 at 459.749 kb on + strand, within Psest_0423 at 459.749 kb on + strand, within Psest_0423 at 459.750 kb on - strand, within Psest_0423 at 459.897 kb on + strand, within Psest_0423 at 459.918 kb on - strand, within Psest_0423 at 459.979 kb on + strand, within Psest_0423 at 459.979 kb on + strand, within Psest_0423 at 459.980 kb on - strand, within Psest_0423 at 460.093 kb on + strand, within Psest_0423 at 460.093 kb on + strand, within Psest_0423 at 460.094 kb on - strand, within Psest_0423 at 460.128 kb on + strand, within Psest_0423 at 460.131 kb on + strand, within Psest_0423 at 460.132 kb on - strand, within Psest_0423 at 460.149 kb on - strand, within Psest_0423 at 460.189 kb on + strand, within Psest_0423 at 460.320 kb on - strand, within Psest_0423 at 460.349 kb on + strand, within Psest_0423 at 460.419 kb on - strand, within Psest_0423 at 460.539 kb on + strand, within Psest_0423 at 460.540 kb on - strand, within Psest_0423 at 460.543 kb on + strand, within Psest_0423 at 460.543 kb on + strand, within Psest_0423 at 460.543 kb on + strand, within Psest_0423 at 460.563 kb on + strand, within Psest_0423 at 460.563 kb on + strand, within Psest_0423 at 460.564 kb on - strand, within Psest_0423 at 460.740 kb on - strand, within Psest_0423 at 460.822 kb on + strand, within Psest_0423 at 460.822 kb on + strand, within Psest_0423 at 460.822 kb on + strand, within Psest_0423 at 460.822 kb on + strand, within Psest_0423 at 460.823 kb on - strand, within Psest_0423 at 460.823 kb on - strand, within Psest_0423 at 460.823 kb on - strand, within Psest_0423 at 460.823 kb on - strand, within Psest_0423 at 460.832 kb on + strand, within Psest_0423 at 460.871 kb on - strand, within Psest_0423 at 460.941 kb on - strand at 460.998 kb on + strand at 461.448 kb on + strand, within Psest_0424 at 461.598 kb on + strand, within Psest_0424 at 461.613 kb on + strand, within Psest_0424 at 461.664 kb on + strand, within Psest_0424 at 461.679 kb on + strand, within Psest_0424 at 461.744 kb on + strand, within Psest_0424 at 461.823 kb on - strand, within Psest_0424 at 461.869 kb on + strand, within Psest_0424 at 461.870 kb on - strand, within Psest_0424 at 461.919 kb on + strand, within Psest_0424 at 461.963 kb on - strand, within Psest_0424 at 461.987 kb on + strand, within Psest_0424 at 462.075 kb on + strand, within Psest_0424 at 462.075 kb on + strand, within Psest_0424
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 458,116 + Psest_0422 0.16 +0.2 458,116 + Psest_0422 0.16 -1.6 458,164 - Psest_0422 0.20 +2.0 458,192 + Psest_0422 0.23 -0.1 458,196 + Psest_0422 0.23 -0.1 458,253 + Psest_0422 0.28 -0.8 458,254 - Psest_0422 0.28 -0.1 458,310 + Psest_0422 0.33 -0.1 458,490 + Psest_0422 0.49 +0.6 458,503 + Psest_0422 0.50 +1.5 458,798 - Psest_0422 0.77 +3.1 458,798 - Psest_0422 0.77 +0.8 458,872 + Psest_0422 0.83 +0.2 458,872 + Psest_0422 0.83 -2.2 458,876 + Psest_0422 0.84 -0.8 458,891 + Psest_0422 0.85 +0.7 459,005 + +0.1 459,006 - -1.5 459,007 + -1.3 459,007 + -0.5 459,008 - -0.3 459,157 - -2.0 459,232 - -0.7 459,232 - -2.7 459,245 + -2.1 459,462 + Psest_0423 0.20 +1.6 459,630 - Psest_0423 0.28 +0.7 459,643 + Psest_0423 0.29 +2.5 459,644 - Psest_0423 0.29 +1.8 459,716 + Psest_0423 0.32 +2.2 459,749 + Psest_0423 0.34 +1.0 459,749 + Psest_0423 0.34 +1.0 459,750 - Psest_0423 0.34 -0.3 459,897 + Psest_0423 0.41 -0.1 459,918 - Psest_0423 0.42 -1.5 459,979 + Psest_0423 0.45 -2.7 459,979 + Psest_0423 0.45 +0.9 459,980 - Psest_0423 0.45 -0.0 460,093 + Psest_0423 0.51 +4.0 460,093 + Psest_0423 0.51 +0.2 460,094 - Psest_0423 0.51 +0.4 460,128 + Psest_0423 0.52 -0.3 460,131 + Psest_0423 0.52 -0.8 460,132 - Psest_0423 0.52 -1.8 460,149 - Psest_0423 0.53 -1.0 460,189 + Psest_0423 0.55 +0.5 460,320 - Psest_0423 0.62 +0.4 460,349 + Psest_0423 0.63 -1.0 460,419 - Psest_0423 0.66 +1.0 460,539 + Psest_0423 0.72 -1.3 460,540 - Psest_0423 0.72 +0.6 460,543 + Psest_0423 0.72 -0.9 460,543 + Psest_0423 0.72 +0.5 460,543 + Psest_0423 0.72 -1.3 460,563 + Psest_0423 0.73 -2.7 460,563 + Psest_0423 0.73 -1.8 460,564 - Psest_0423 0.73 +1.6 460,740 - Psest_0423 0.82 +0.7 460,822 + Psest_0423 0.86 +0.8 460,822 + Psest_0423 0.86 -1.6 460,822 + Psest_0423 0.86 +0.9 460,822 + Psest_0423 0.86 -1.1 460,823 - Psest_0423 0.86 -0.3 460,823 - Psest_0423 0.86 +2.2 460,823 - Psest_0423 0.86 +1.2 460,823 - Psest_0423 0.86 +1.2 460,832 + Psest_0423 0.86 +1.4 460,871 - Psest_0423 0.88 +0.3 460,941 - +0.4 460,998 + -0.3 461,448 + Psest_0424 0.18 +0.7 461,598 + Psest_0424 0.26 -0.5 461,613 + Psest_0424 0.27 -0.1 461,664 + Psest_0424 0.30 -0.3 461,679 + Psest_0424 0.31 -0.2 461,744 + Psest_0424 0.34 -2.6 461,823 - Psest_0424 0.38 -2.5 461,869 + Psest_0424 0.41 -0.3 461,870 - Psest_0424 0.41 +0.6 461,919 + Psest_0424 0.44 +0.9 461,963 - Psest_0424 0.46 -0.7 461,987 + Psest_0424 0.47 -0.8 462,075 + Psest_0424 0.52 +1.1 462,075 + Psest_0424 0.52 -0.2
Or see this region's nucleotide sequence