Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3998 and Psest_3999 are separated by 209 nucleotides Psest_3999 and Psest_4000 are separated by 34 nucleotides
Psest_3998: Psest_3998 - glutamate--cysteine ligase, at 4,168,047 to 4,169,624
_3998
Psest_3999: Psest_3999 - Acetyl esterase (deacetylase), at 4,169,834 to 4,171,570
_3999
Psest_4000: Psest_4000 - amino-acid N-acetyltransferase, at 4,171,605 to 4,172,903
_4000
Position (kb)
4169
4170
4171
4172 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4168.860 kb on + strand, within Psest_3998 at 4168.947 kb on + strand, within Psest_3998 at 4168.947 kb on + strand, within Psest_3998 at 4168.947 kb on + strand, within Psest_3998 at 4168.947 kb on + strand, within Psest_3998 at 4168.950 kb on + strand, within Psest_3998 at 4168.951 kb on - strand, within Psest_3998 at 4169.028 kb on + strand, within Psest_3998 at 4169.028 kb on + strand, within Psest_3998 at 4169.029 kb on - strand, within Psest_3998 at 4169.029 kb on - strand, within Psest_3998 at 4169.029 kb on - strand, within Psest_3998 at 4169.029 kb on - strand, within Psest_3998 at 4169.038 kb on + strand, within Psest_3998 at 4169.038 kb on + strand, within Psest_3998 at 4169.039 kb on - strand, within Psest_3998 at 4169.039 kb on - strand, within Psest_3998 at 4169.039 kb on - strand, within Psest_3998 at 4169.039 kb on - strand, within Psest_3998 at 4169.649 kb on + strand at 4169.649 kb on + strand at 4169.649 kb on + strand at 4169.649 kb on + strand at 4169.649 kb on + strand at 4169.649 kb on + strand at 4169.649 kb on + strand at 4169.649 kb on + strand at 4169.650 kb on - strand at 4169.650 kb on - strand at 4169.650 kb on - strand at 4169.650 kb on - strand at 4169.650 kb on - strand at 4169.650 kb on - strand at 4169.650 kb on - strand at 4169.650 kb on - strand at 4169.650 kb on - strand at 4169.650 kb on - strand at 4169.652 kb on - strand at 4169.661 kb on + strand at 4169.662 kb on - strand at 4169.662 kb on - strand at 4169.670 kb on + strand at 4169.671 kb on - strand at 4169.671 kb on - strand at 4169.677 kb on + strand at 4169.678 kb on - strand at 4169.678 kb on - strand at 4169.678 kb on - strand at 4169.678 kb on - strand at 4170.089 kb on + strand, within Psest_3999 at 4170.089 kb on + strand, within Psest_3999 at 4170.089 kb on + strand, within Psest_3999 at 4170.089 kb on + strand, within Psest_3999 at 4170.090 kb on - strand, within Psest_3999 at 4170.090 kb on - strand, within Psest_3999 at 4170.090 kb on - strand, within Psest_3999 at 4170.090 kb on - strand, within Psest_3999 at 4170.090 kb on - strand, within Psest_3999 at 4170.090 kb on - strand, within Psest_3999 at 4170.090 kb on - strand, within Psest_3999 at 4170.090 kb on - strand, within Psest_3999 at 4170.090 kb on - strand, within Psest_3999 at 4170.090 kb on - strand, within Psest_3999 at 4170.090 kb on - strand, within Psest_3999 at 4170.179 kb on + strand, within Psest_3999 at 4170.179 kb on + strand, within Psest_3999 at 4170.180 kb on - strand, within Psest_3999 at 4170.318 kb on - strand, within Psest_3999 at 4170.446 kb on + strand, within Psest_3999 at 4170.447 kb on - strand, within Psest_3999 at 4170.542 kb on + strand, within Psest_3999 at 4170.704 kb on + strand, within Psest_3999 at 4170.704 kb on + strand, within Psest_3999 at 4170.704 kb on + strand, within Psest_3999 at 4170.704 kb on + strand, within Psest_3999 at 4170.704 kb on + strand, within Psest_3999 at 4170.704 kb on + strand, within Psest_3999 at 4170.704 kb on + strand, within Psest_3999 at 4170.705 kb on - strand, within Psest_3999 at 4170.705 kb on - strand, within Psest_3999 at 4170.705 kb on - strand, within Psest_3999 at 4170.705 kb on - strand, within Psest_3999 at 4170.705 kb on - strand, within Psest_3999 at 4170.705 kb on - strand, within Psest_3999 at 4170.705 kb on - strand, within Psest_3999 at 4170.705 kb on - strand, within Psest_3999 at 4170.737 kb on + strand, within Psest_3999 at 4170.737 kb on + strand, within Psest_3999 at 4170.738 kb on - strand, within Psest_3999 at 4170.738 kb on - strand, within Psest_3999 at 4170.738 kb on - strand, within Psest_3999 at 4170.813 kb on + strand, within Psest_3999 at 4170.833 kb on + strand, within Psest_3999 at 4170.834 kb on - strand, within Psest_3999 at 4170.854 kb on + strand, within Psest_3999 at 4170.945 kb on + strand, within Psest_3999 at 4170.945 kb on + strand, within Psest_3999 at 4170.946 kb on - strand, within Psest_3999 at 4170.946 kb on - strand, within Psest_3999 at 4170.946 kb on - strand, within Psest_3999 at 4171.051 kb on - strand, within Psest_3999 at 4171.051 kb on - strand, within Psest_3999 at 4171.234 kb on - strand, within Psest_3999 at 4171.241 kb on + strand, within Psest_3999 at 4171.241 kb on + strand, within Psest_3999 at 4171.242 kb on - strand, within Psest_3999 at 4171.242 kb on - strand, within Psest_3999 at 4171.482 kb on - strand at 4171.499 kb on + strand at 4171.500 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 4,168,860 + Psest_3998 0.52 +0.8 4,168,947 + Psest_3998 0.57 +2.6 4,168,947 + Psest_3998 0.57 +0.9 4,168,947 + Psest_3998 0.57 +0.5 4,168,947 + Psest_3998 0.57 +3.6 4,168,950 + Psest_3998 0.57 -0.8 4,168,951 - Psest_3998 0.57 +2.7 4,169,028 + Psest_3998 0.62 -1.8 4,169,028 + Psest_3998 0.62 +2.0 4,169,029 - Psest_3998 0.62 +0.7 4,169,029 - Psest_3998 0.62 +1.9 4,169,029 - Psest_3998 0.62 -2.3 4,169,029 - Psest_3998 0.62 +2.3 4,169,038 + Psest_3998 0.63 +0.3 4,169,038 + Psest_3998 0.63 -1.1 4,169,039 - Psest_3998 0.63 +0.1 4,169,039 - Psest_3998 0.63 -1.0 4,169,039 - Psest_3998 0.63 +2.4 4,169,039 - Psest_3998 0.63 +3.8 4,169,649 + -0.7 4,169,649 + -0.7 4,169,649 + +0.9 4,169,649 + -3.2 4,169,649 + -1.8 4,169,649 + -0.7 4,169,649 + +0.9 4,169,649 + +2.6 4,169,650 - +1.0 4,169,650 - -0.8 4,169,650 - -0.9 4,169,650 - +0.7 4,169,650 - +1.5 4,169,650 - +1.0 4,169,650 - +2.2 4,169,650 - -0.7 4,169,650 - +1.1 4,169,650 - +0.2 4,169,652 - -0.6 4,169,661 + -0.5 4,169,662 - -0.1 4,169,662 - -1.8 4,169,670 + +0.1 4,169,671 - +0.4 4,169,671 - -1.1 4,169,677 + -0.1 4,169,678 - -0.1 4,169,678 - -0.5 4,169,678 - -0.1 4,169,678 - -1.4 4,170,089 + Psest_3999 0.15 +0.8 4,170,089 + Psest_3999 0.15 -0.9 4,170,089 + Psest_3999 0.15 +0.9 4,170,089 + Psest_3999 0.15 -0.5 4,170,090 - Psest_3999 0.15 +1.4 4,170,090 - Psest_3999 0.15 -2.1 4,170,090 - Psest_3999 0.15 -1.3 4,170,090 - Psest_3999 0.15 -0.9 4,170,090 - Psest_3999 0.15 +1.4 4,170,090 - Psest_3999 0.15 +1.1 4,170,090 - Psest_3999 0.15 +0.5 4,170,090 - Psest_3999 0.15 -1.7 4,170,090 - Psest_3999 0.15 -0.2 4,170,090 - Psest_3999 0.15 +0.1 4,170,090 - Psest_3999 0.15 +0.8 4,170,179 + Psest_3999 0.20 +1.3 4,170,179 + Psest_3999 0.20 +0.7 4,170,180 - Psest_3999 0.20 -2.7 4,170,318 - Psest_3999 0.28 -0.1 4,170,446 + Psest_3999 0.35 -0.5 4,170,447 - Psest_3999 0.35 -1.3 4,170,542 + Psest_3999 0.41 -0.8 4,170,704 + Psest_3999 0.50 +0.4 4,170,704 + Psest_3999 0.50 -1.9 4,170,704 + Psest_3999 0.50 +1.1 4,170,704 + Psest_3999 0.50 -0.9 4,170,704 + Psest_3999 0.50 -0.5 4,170,704 + Psest_3999 0.50 -0.5 4,170,704 + Psest_3999 0.50 +0.9 4,170,705 - Psest_3999 0.50 -0.8 4,170,705 - Psest_3999 0.50 +0.2 4,170,705 - Psest_3999 0.50 +0.6 4,170,705 - Psest_3999 0.50 -0.5 4,170,705 - Psest_3999 0.50 -0.1 4,170,705 - Psest_3999 0.50 -0.1 4,170,705 - Psest_3999 0.50 -2.3 4,170,705 - Psest_3999 0.50 -1.7 4,170,737 + Psest_3999 0.52 -0.5 4,170,737 + Psest_3999 0.52 -0.7 4,170,738 - Psest_3999 0.52 -2.1 4,170,738 - Psest_3999 0.52 -0.8 4,170,738 - Psest_3999 0.52 -1.8 4,170,813 + Psest_3999 0.56 +1.3 4,170,833 + Psest_3999 0.58 -2.5 4,170,834 - Psest_3999 0.58 -1.2 4,170,854 + Psest_3999 0.59 -1.1 4,170,945 + Psest_3999 0.64 +0.8 4,170,945 + Psest_3999 0.64 -1.9 4,170,946 - Psest_3999 0.64 -1.9 4,170,946 - Psest_3999 0.64 -0.5 4,170,946 - Psest_3999 0.64 -3.1 4,171,051 - Psest_3999 0.70 -1.7 4,171,051 - Psest_3999 0.70 -0.2 4,171,234 - Psest_3999 0.81 -0.7 4,171,241 + Psest_3999 0.81 +0.0 4,171,241 + Psest_3999 0.81 -0.3 4,171,242 - Psest_3999 0.81 -0.5 4,171,242 - Psest_3999 0.81 -2.2 4,171,482 - -0.3 4,171,499 + -0.7 4,171,500 - +0.5
Or see this region's nucleotide sequence