Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3333 and Psest_3334 are separated by 40 nucleotides Psest_3334 and Psest_3336 are separated by 353 nucleotides Psest_3336 and Psest_3337 are separated by 184 nucleotides
Psest_3333: Psest_3333 - K+-dependent Na+/Ca+ exchanger related-protein, at 3,498,647 to 3,499,735
_3333
Psest_3334: Psest_3334 - Integral membrane protein CcmA involved in cell shape determination, at 3,499,776 to 3,500,198
_3334
Psest_3336: Psest_3336 - Membrane proteins related to metalloendopeptidases, at 3,500,552 to 3,501,535
_3336
Psest_3337: Psest_3337 - Predicted permeases, at 3,501,720 to 3,502,661
_3337
Position (kb)
3500
3501
3502 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3499.580 kb on + strand, within Psest_3333 at 3499.580 kb on + strand, within Psest_3333 at 3499.580 kb on + strand, within Psest_3333 at 3499.583 kb on + strand, within Psest_3333 at 3499.583 kb on + strand, within Psest_3333 at 3499.583 kb on + strand, within Psest_3333 at 3499.584 kb on - strand, within Psest_3333 at 3499.584 kb on - strand, within Psest_3333 at 3499.584 kb on - strand, within Psest_3333 at 3499.592 kb on + strand, within Psest_3333 at 3499.592 kb on + strand, within Psest_3333 at 3499.593 kb on - strand, within Psest_3333 at 3499.593 kb on - strand, within Psest_3333 at 3499.601 kb on + strand, within Psest_3333 at 3499.601 kb on + strand, within Psest_3333 at 3499.664 kb on + strand at 3499.774 kb on - strand at 3499.990 kb on + strand, within Psest_3334 at 3499.990 kb on + strand, within Psest_3334 at 3499.991 kb on - strand, within Psest_3334 at 3499.991 kb on - strand, within Psest_3334 at 3499.991 kb on - strand, within Psest_3334 at 3499.991 kb on - strand, within Psest_3334 at 3499.995 kb on - strand, within Psest_3334 at 3499.995 kb on - strand, within Psest_3334 at 3500.028 kb on + strand, within Psest_3334 at 3500.029 kb on - strand, within Psest_3334 at 3500.203 kb on + strand at 3500.203 kb on + strand at 3500.204 kb on - strand at 3500.204 kb on - strand at 3500.204 kb on - strand at 3500.204 kb on - strand at 3500.435 kb on + strand at 3500.435 kb on + strand at 3500.436 kb on - strand at 3500.436 kb on - strand at 3500.554 kb on - strand at 3500.554 kb on - strand at 3500.554 kb on - strand at 3500.684 kb on + strand, within Psest_3336 at 3500.729 kb on + strand, within Psest_3336 at 3500.729 kb on + strand, within Psest_3336 at 3500.729 kb on + strand, within Psest_3336 at 3500.729 kb on + strand, within Psest_3336 at 3500.729 kb on + strand, within Psest_3336 at 3501.035 kb on + strand, within Psest_3336 at 3501.036 kb on - strand, within Psest_3336 at 3501.303 kb on - strand, within Psest_3336 at 3501.431 kb on + strand, within Psest_3336 at 3501.431 kb on + strand, within Psest_3336 at 3501.431 kb on + strand, within Psest_3336 at 3501.431 kb on + strand, within Psest_3336 at 3501.432 kb on - strand, within Psest_3336 at 3501.432 kb on - strand, within Psest_3336 at 3501.432 kb on - strand, within Psest_3336 at 3501.432 kb on - strand, within Psest_3336 at 3501.432 kb on - strand, within Psest_3336 at 3501.517 kb on + strand at 3501.517 kb on + strand at 3501.517 kb on + strand at 3501.517 kb on + strand at 3501.518 kb on - strand at 3501.518 kb on - strand at 3501.518 kb on - strand at 3501.547 kb on + strand at 3501.554 kb on + strand at 3501.665 kb on + strand at 3501.721 kb on + strand at 3501.721 kb on + strand at 3501.721 kb on + strand at 3501.722 kb on - strand at 3501.722 kb on - strand at 3501.722 kb on - strand at 3501.722 kb on - strand at 3501.722 kb on - strand at 3501.877 kb on + strand, within Psest_3337 at 3501.878 kb on - strand, within Psest_3337 at 3501.878 kb on - strand, within Psest_3337 at 3502.038 kb on + strand, within Psest_3337 at 3502.069 kb on + strand, within Psest_3337 at 3502.070 kb on - strand, within Psest_3337 at 3502.114 kb on + strand, within Psest_3337 at 3502.115 kb on - strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.210 kb on + strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.211 kb on - strand, within Psest_3337 at 3502.243 kb on + strand, within Psest_3337 at 3502.243 kb on + strand, within Psest_3337 at 3502.243 kb on + strand, within Psest_3337 at 3502.243 kb on + strand, within Psest_3337 at 3502.243 kb on + strand, within Psest_3337 at 3502.244 kb on - strand, within Psest_3337 at 3502.244 kb on - strand, within Psest_3337 at 3502.244 kb on - strand, within Psest_3337 at 3502.301 kb on - strand, within Psest_3337 at 3502.301 kb on - strand, within Psest_3337 at 3502.363 kb on + strand, within Psest_3337 at 3502.363 kb on + strand, within Psest_3337 at 3502.363 kb on + strand, within Psest_3337 at 3502.363 kb on + strand, within Psest_3337 at 3502.363 kb on + strand, within Psest_3337 at 3502.364 kb on - strand, within Psest_3337 at 3502.447 kb on + strand, within Psest_3337 at 3502.447 kb on + strand, within Psest_3337 at 3502.447 kb on + strand, within Psest_3337 at 3502.448 kb on - strand, within Psest_3337 at 3502.448 kb on - strand, within Psest_3337 at 3502.502 kb on + strand, within Psest_3337 at 3502.503 kb on - strand, within Psest_3337 at 3502.503 kb on - strand, within Psest_3337
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 3,499,580 + Psest_3333 0.86 +0.8 3,499,580 + Psest_3333 0.86 -0.2 3,499,580 + Psest_3333 0.86 -2.2 3,499,583 + Psest_3333 0.86 +1.6 3,499,583 + Psest_3333 0.86 +3.6 3,499,583 + Psest_3333 0.86 -2.0 3,499,584 - Psest_3333 0.86 -0.5 3,499,584 - Psest_3333 0.86 -1.0 3,499,584 - Psest_3333 0.86 -1.2 3,499,592 + Psest_3333 0.87 -0.6 3,499,592 + Psest_3333 0.87 +0.1 3,499,593 - Psest_3333 0.87 +2.2 3,499,593 - Psest_3333 0.87 -0.3 3,499,601 + Psest_3333 0.88 +0.4 3,499,601 + Psest_3333 0.88 +0.3 3,499,664 + +1.1 3,499,774 - -0.4 3,499,990 + Psest_3334 0.51 +0.4 3,499,990 + Psest_3334 0.51 +0.9 3,499,991 - Psest_3334 0.51 +2.2 3,499,991 - Psest_3334 0.51 -0.3 3,499,991 - Psest_3334 0.51 -2.4 3,499,991 - Psest_3334 0.51 +0.5 3,499,995 - Psest_3334 0.52 -2.4 3,499,995 - Psest_3334 0.52 -0.8 3,500,028 + Psest_3334 0.60 -0.2 3,500,029 - Psest_3334 0.60 -1.0 3,500,203 + +0.7 3,500,203 + +1.4 3,500,204 - +1.1 3,500,204 - -0.6 3,500,204 - +0.8 3,500,204 - +0.5 3,500,435 + -2.5 3,500,435 + -1.5 3,500,436 - -0.2 3,500,436 - -0.4 3,500,554 - -1.5 3,500,554 - -0.6 3,500,554 - +3.0 3,500,684 + Psest_3336 0.13 +1.1 3,500,729 + Psest_3336 0.18 -0.5 3,500,729 + Psest_3336 0.18 +0.3 3,500,729 + Psest_3336 0.18 -0.5 3,500,729 + Psest_3336 0.18 +1.0 3,500,729 + Psest_3336 0.18 +0.2 3,501,035 + Psest_3336 0.49 -0.6 3,501,036 - Psest_3336 0.49 +0.4 3,501,303 - Psest_3336 0.76 +3.1 3,501,431 + Psest_3336 0.89 +0.5 3,501,431 + Psest_3336 0.89 -1.3 3,501,431 + Psest_3336 0.89 +1.2 3,501,431 + Psest_3336 0.89 +0.4 3,501,432 - Psest_3336 0.89 +0.0 3,501,432 - Psest_3336 0.89 -2.0 3,501,432 - Psest_3336 0.89 -2.7 3,501,432 - Psest_3336 0.89 -1.0 3,501,432 - Psest_3336 0.89 -0.4 3,501,517 + -2.4 3,501,517 + +1.0 3,501,517 + +0.9 3,501,517 + +0.4 3,501,518 - -1.4 3,501,518 - +0.8 3,501,518 - -2.3 3,501,547 + +1.1 3,501,554 + -1.3 3,501,665 + -0.6 3,501,721 + +0.5 3,501,721 + +0.4 3,501,721 + +1.0 3,501,722 - +1.4 3,501,722 - +0.2 3,501,722 - -0.7 3,501,722 - +0.8 3,501,722 - +0.7 3,501,877 + Psest_3337 0.17 -0.4 3,501,878 - Psest_3337 0.17 +2.1 3,501,878 - Psest_3337 0.17 -0.1 3,502,038 + Psest_3337 0.34 +1.3 3,502,069 + Psest_3337 0.37 +0.7 3,502,070 - Psest_3337 0.37 +0.6 3,502,114 + Psest_3337 0.42 +1.0 3,502,115 - Psest_3337 0.42 +1.3 3,502,210 + Psest_3337 0.52 -1.9 3,502,210 + Psest_3337 0.52 +1.2 3,502,210 + Psest_3337 0.52 +0.3 3,502,210 + Psest_3337 0.52 -0.6 3,502,210 + Psest_3337 0.52 -2.6 3,502,210 + Psest_3337 0.52 +1.9 3,502,210 + Psest_3337 0.52 +0.4 3,502,210 + Psest_3337 0.52 +0.9 3,502,210 + Psest_3337 0.52 +0.1 3,502,210 + Psest_3337 0.52 +2.1 3,502,210 + Psest_3337 0.52 -0.9 3,502,210 + Psest_3337 0.52 -0.6 3,502,210 + Psest_3337 0.52 +0.2 3,502,210 + Psest_3337 0.52 -1.2 3,502,210 + Psest_3337 0.52 +0.6 3,502,210 + Psest_3337 0.52 +0.9 3,502,210 + Psest_3337 0.52 +0.2 3,502,210 + Psest_3337 0.52 -0.0 3,502,210 + Psest_3337 0.52 +1.4 3,502,210 + Psest_3337 0.52 +0.3 3,502,211 - Psest_3337 0.52 +1.2 3,502,211 - Psest_3337 0.52 +1.5 3,502,211 - Psest_3337 0.52 +0.3 3,502,211 - Psest_3337 0.52 -2.0 3,502,211 - Psest_3337 0.52 +1.0 3,502,211 - Psest_3337 0.52 +0.6 3,502,211 - Psest_3337 0.52 -0.4 3,502,211 - Psest_3337 0.52 +2.9 3,502,211 - Psest_3337 0.52 -0.3 3,502,211 - Psest_3337 0.52 +0.1 3,502,211 - Psest_3337 0.52 +0.7 3,502,211 - Psest_3337 0.52 +1.1 3,502,211 - Psest_3337 0.52 -2.5 3,502,211 - Psest_3337 0.52 +2.3 3,502,211 - Psest_3337 0.52 -1.0 3,502,211 - Psest_3337 0.52 -0.3 3,502,243 + Psest_3337 0.56 +1.7 3,502,243 + Psest_3337 0.56 -2.8 3,502,243 + Psest_3337 0.56 +0.9 3,502,243 + Psest_3337 0.56 -1.2 3,502,243 + Psest_3337 0.56 +0.8 3,502,244 - Psest_3337 0.56 -0.4 3,502,244 - Psest_3337 0.56 +0.6 3,502,244 - Psest_3337 0.56 +1.4 3,502,301 - Psest_3337 0.62 +0.3 3,502,301 - Psest_3337 0.62 +0.1 3,502,363 + Psest_3337 0.68 -1.0 3,502,363 + Psest_3337 0.68 -2.0 3,502,363 + Psest_3337 0.68 +0.1 3,502,363 + Psest_3337 0.68 +1.4 3,502,363 + Psest_3337 0.68 -0.7 3,502,364 - Psest_3337 0.68 +0.2 3,502,447 + Psest_3337 0.77 +0.2 3,502,447 + Psest_3337 0.77 +1.0 3,502,447 + Psest_3337 0.77 -2.0 3,502,448 - Psest_3337 0.77 -1.2 3,502,448 - Psest_3337 0.77 +1.4 3,502,502 + Psest_3337 0.83 +1.2 3,502,503 - Psest_3337 0.83 +1.0 3,502,503 - Psest_3337 0.83 -0.8
Or see this region's nucleotide sequence