Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2638 and Psest_2639 are separated by 80 nucleotides Psest_2639 and Psest_2640 are separated by 7 nucleotides Psest_2640 and Psest_2641 are separated by 7 nucleotides Psest_2641 and Psest_2642 are separated by 146 nucleotides Psest_2642 and Psest_2643 are separated by 24 nucleotides
Psest_2638: Psest_2638 - Protein related to penicillin acylase, at 2,821,888 to 2,824,425
_2638
Psest_2639: Psest_2639 - Protein of unknown function (DUF2489)., at 2,824,506 to 2,824,967
_2639
Psest_2640: Psest_2640 - Preprotein translocase subunit SecA (ATPase, RNA helicase), at 2,824,975 to 2,825,163
_2640
Psest_2641: Psest_2641 - Conserved secreted protein, at 2,825,171 to 2,825,668
_2641
Psest_2642: Psest_2642 - 5'-deoxy-5'-methylthioadenosine phosphorylase, at 2,825,815 to 2,826,552
_2642
Psest_2643: Psest_2643 - Beta-glucosidase-related glycosidases, at 2,826,577 to 2,827,575
_2643
Position (kb)
2825
2826 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2824.187 kb on + strand at 2824.188 kb on - strand at 2824.188 kb on - strand at 2824.325 kb on + strand at 2824.341 kb on - strand at 2824.474 kb on + strand at 2824.475 kb on - strand at 2824.475 kb on - strand at 2824.577 kb on + strand, within Psest_2639 at 2824.577 kb on + strand, within Psest_2639 at 2824.577 kb on + strand, within Psest_2639 at 2824.577 kb on + strand, within Psest_2639 at 2824.577 kb on + strand, within Psest_2639 at 2824.578 kb on - strand, within Psest_2639 at 2824.578 kb on - strand, within Psest_2639 at 2824.578 kb on - strand, within Psest_2639 at 2824.578 kb on - strand, within Psest_2639 at 2824.732 kb on + strand, within Psest_2639 at 2824.732 kb on + strand, within Psest_2639 at 2824.732 kb on + strand, within Psest_2639 at 2824.732 kb on + strand, within Psest_2639 at 2824.732 kb on + strand, within Psest_2639 at 2824.733 kb on - strand, within Psest_2639 at 2824.733 kb on - strand, within Psest_2639 at 2824.733 kb on - strand, within Psest_2639 at 2824.736 kb on + strand, within Psest_2639 at 2824.736 kb on + strand, within Psest_2639 at 2824.736 kb on + strand, within Psest_2639 at 2824.736 kb on + strand, within Psest_2639 at 2824.736 kb on + strand, within Psest_2639 at 2824.736 kb on + strand, within Psest_2639 at 2824.737 kb on - strand, within Psest_2639 at 2824.737 kb on - strand, within Psest_2639 at 2824.737 kb on - strand, within Psest_2639 at 2824.740 kb on + strand, within Psest_2639 at 2824.897 kb on + strand, within Psest_2639 at 2824.897 kb on + strand, within Psest_2639 at 2824.897 kb on + strand, within Psest_2639 at 2824.898 kb on - strand, within Psest_2639 at 2825.172 kb on + strand at 2825.172 kb on + strand at 2825.172 kb on + strand at 2825.173 kb on - strand at 2825.173 kb on - strand at 2825.198 kb on + strand at 2825.199 kb on - strand at 2825.199 kb on - strand at 2825.199 kb on - strand at 2825.313 kb on + strand, within Psest_2641 at 2825.313 kb on + strand, within Psest_2641 at 2825.313 kb on + strand, within Psest_2641 at 2825.313 kb on + strand, within Psest_2641 at 2825.313 kb on + strand, within Psest_2641 at 2825.313 kb on + strand, within Psest_2641 at 2825.313 kb on + strand, within Psest_2641 at 2825.313 kb on + strand, within Psest_2641 at 2825.314 kb on - strand, within Psest_2641 at 2825.314 kb on - strand, within Psest_2641 at 2825.314 kb on - strand, within Psest_2641 at 2825.314 kb on - strand, within Psest_2641 at 2825.314 kb on - strand, within Psest_2641 at 2825.314 kb on - strand, within Psest_2641 at 2825.314 kb on - strand, within Psest_2641 at 2825.314 kb on - strand, within Psest_2641 at 2825.314 kb on - strand, within Psest_2641 at 2825.314 kb on - strand, within Psest_2641 at 2825.314 kb on - strand, within Psest_2641 at 2825.314 kb on - strand, within Psest_2641 at 2825.355 kb on + strand, within Psest_2641 at 2825.426 kb on + strand, within Psest_2641 at 2825.427 kb on - strand, within Psest_2641 at 2825.438 kb on - strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.439 kb on + strand, within Psest_2641 at 2825.440 kb on - strand, within Psest_2641 at 2825.440 kb on - strand, within Psest_2641 at 2825.440 kb on - strand, within Psest_2641 at 2825.440 kb on - strand, within Psest_2641 at 2825.440 kb on - strand, within Psest_2641 at 2825.440 kb on - strand, within Psest_2641 at 2825.440 kb on - strand, within Psest_2641 at 2825.522 kb on - strand, within Psest_2641 at 2825.522 kb on - strand, within Psest_2641 at 2825.630 kb on + strand at 2825.635 kb on + strand at 2825.635 kb on + strand at 2825.645 kb on + strand at 2825.645 kb on + strand at 2825.645 kb on + strand at 2825.645 kb on + strand at 2825.645 kb on + strand at 2825.645 kb on + strand at 2825.646 kb on - strand at 2825.646 kb on - strand at 2825.646 kb on - strand at 2825.646 kb on - strand at 2825.696 kb on + strand at 2825.696 kb on + strand at 2825.697 kb on - strand at 2825.697 kb on - strand at 2825.739 kb on + strand at 2825.739 kb on + strand at 2825.758 kb on + strand at 2825.759 kb on - strand at 2825.951 kb on + strand, within Psest_2642 at 2825.952 kb on - strand, within Psest_2642 at 2825.991 kb on + strand, within Psest_2642 at 2825.991 kb on + strand, within Psest_2642 at 2826.000 kb on + strand, within Psest_2642 at 2826.001 kb on - strand, within Psest_2642 at 2826.081 kb on - strand, within Psest_2642 at 2826.081 kb on - strand, within Psest_2642 at 2826.081 kb on - strand, within Psest_2642 at 2826.156 kb on - strand, within Psest_2642 at 2826.174 kb on + strand, within Psest_2642 at 2826.224 kb on + strand, within Psest_2642 at 2826.224 kb on + strand, within Psest_2642 at 2826.224 kb on + strand, within Psest_2642 at 2826.224 kb on + strand, within Psest_2642 at 2826.224 kb on + strand, within Psest_2642 at 2826.224 kb on + strand, within Psest_2642 at 2826.224 kb on + strand, within Psest_2642 at 2826.225 kb on - strand, within Psest_2642 at 2826.225 kb on - strand, within Psest_2642 at 2826.225 kb on - strand, within Psest_2642 at 2826.225 kb on - strand, within Psest_2642 at 2826.225 kb on - strand, within Psest_2642 at 2826.225 kb on - strand, within Psest_2642 at 2826.225 kb on - strand, within Psest_2642 at 2826.225 kb on - strand, within Psest_2642 at 2826.225 kb on - strand, within Psest_2642 at 2826.225 kb on - strand, within Psest_2642 at 2826.225 kb on - strand, within Psest_2642 at 2826.543 kb on - strand at 2826.543 kb on - strand at 2826.556 kb on + strand at 2826.642 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,824,187 + +2.4 2,824,188 - +0.6 2,824,188 - +0.3 2,824,325 + +1.4 2,824,341 - -1.8 2,824,474 + -1.0 2,824,475 - -1.3 2,824,475 - -2.4 2,824,577 + Psest_2639 0.15 -0.4 2,824,577 + Psest_2639 0.15 +1.6 2,824,577 + Psest_2639 0.15 +0.7 2,824,577 + Psest_2639 0.15 -1.9 2,824,577 + Psest_2639 0.15 +0.3 2,824,578 - Psest_2639 0.16 -0.2 2,824,578 - Psest_2639 0.16 +2.1 2,824,578 - Psest_2639 0.16 -3.1 2,824,578 - Psest_2639 0.16 -0.3 2,824,732 + Psest_2639 0.49 +0.6 2,824,732 + Psest_2639 0.49 -0.0 2,824,732 + Psest_2639 0.49 -0.8 2,824,732 + Psest_2639 0.49 +1.8 2,824,732 + Psest_2639 0.49 +3.1 2,824,733 - Psest_2639 0.49 +0.3 2,824,733 - Psest_2639 0.49 -1.3 2,824,733 - Psest_2639 0.49 +1.9 2,824,736 + Psest_2639 0.50 +0.0 2,824,736 + Psest_2639 0.50 +1.0 2,824,736 + Psest_2639 0.50 +0.6 2,824,736 + Psest_2639 0.50 -0.4 2,824,736 + Psest_2639 0.50 +0.4 2,824,736 + Psest_2639 0.50 -0.8 2,824,737 - Psest_2639 0.50 +1.7 2,824,737 - Psest_2639 0.50 +0.1 2,824,737 - Psest_2639 0.50 +0.3 2,824,740 + Psest_2639 0.51 -1.7 2,824,897 + Psest_2639 0.85 -1.3 2,824,897 + Psest_2639 0.85 -1.1 2,824,897 + Psest_2639 0.85 -1.5 2,824,898 - Psest_2639 0.85 +1.0 2,825,172 + +0.2 2,825,172 + +0.0 2,825,172 + +1.4 2,825,173 - +1.2 2,825,173 - +1.1 2,825,198 + +1.4 2,825,199 - +0.8 2,825,199 - +2.4 2,825,199 - +1.5 2,825,313 + Psest_2641 0.29 +1.6 2,825,313 + Psest_2641 0.29 +2.0 2,825,313 + Psest_2641 0.29 +1.3 2,825,313 + Psest_2641 0.29 +0.9 2,825,313 + Psest_2641 0.29 +1.2 2,825,313 + Psest_2641 0.29 +0.7 2,825,313 + Psest_2641 0.29 +1.7 2,825,313 + Psest_2641 0.29 +2.0 2,825,314 - Psest_2641 0.29 +2.1 2,825,314 - Psest_2641 0.29 +1.3 2,825,314 - Psest_2641 0.29 +0.6 2,825,314 - Psest_2641 0.29 +0.5 2,825,314 - Psest_2641 0.29 +1.8 2,825,314 - Psest_2641 0.29 -0.1 2,825,314 - Psest_2641 0.29 +1.8 2,825,314 - Psest_2641 0.29 +1.7 2,825,314 - Psest_2641 0.29 +0.7 2,825,314 - Psest_2641 0.29 +1.9 2,825,314 - Psest_2641 0.29 +0.7 2,825,314 - Psest_2641 0.29 +1.7 2,825,355 + Psest_2641 0.37 +0.3 2,825,426 + Psest_2641 0.51 +2.1 2,825,427 - Psest_2641 0.51 +2.6 2,825,438 - Psest_2641 0.54 +1.6 2,825,439 + Psest_2641 0.54 +1.9 2,825,439 + Psest_2641 0.54 +1.2 2,825,439 + Psest_2641 0.54 +0.1 2,825,439 + Psest_2641 0.54 +0.9 2,825,439 + Psest_2641 0.54 +2.1 2,825,439 + Psest_2641 0.54 -0.1 2,825,439 + Psest_2641 0.54 +0.6 2,825,439 + Psest_2641 0.54 +2.1 2,825,439 + Psest_2641 0.54 +0.7 2,825,439 + Psest_2641 0.54 +1.5 2,825,439 + Psest_2641 0.54 -0.2 2,825,439 + Psest_2641 0.54 +0.5 2,825,439 + Psest_2641 0.54 +1.1 2,825,439 + Psest_2641 0.54 +1.0 2,825,439 + Psest_2641 0.54 +1.8 2,825,440 - Psest_2641 0.54 +1.0 2,825,440 - Psest_2641 0.54 +2.6 2,825,440 - Psest_2641 0.54 +0.3 2,825,440 - Psest_2641 0.54 -2.4 2,825,440 - Psest_2641 0.54 +0.3 2,825,440 - Psest_2641 0.54 +0.6 2,825,440 - Psest_2641 0.54 +0.5 2,825,522 - Psest_2641 0.70 +1.6 2,825,522 - Psest_2641 0.70 -0.3 2,825,630 + +1.7 2,825,635 + +1.3 2,825,635 + +2.6 2,825,645 + +1.2 2,825,645 + +2.3 2,825,645 + +2.6 2,825,645 + +1.8 2,825,645 + +0.1 2,825,645 + +1.5 2,825,646 - +1.6 2,825,646 - +1.0 2,825,646 - +2.0 2,825,646 - +2.2 2,825,696 + +1.7 2,825,696 + +0.1 2,825,697 - +0.0 2,825,697 - -0.9 2,825,739 + +1.8 2,825,739 + +1.5 2,825,758 + +1.1 2,825,759 - -0.8 2,825,951 + Psest_2642 0.18 -0.4 2,825,952 - Psest_2642 0.19 -2.3 2,825,991 + Psest_2642 0.24 -1.7 2,825,991 + Psest_2642 0.24 +0.3 2,826,000 + Psest_2642 0.25 +0.0 2,826,001 - Psest_2642 0.25 +0.3 2,826,081 - Psest_2642 0.36 -0.2 2,826,081 - Psest_2642 0.36 +0.1 2,826,081 - Psest_2642 0.36 -1.8 2,826,156 - Psest_2642 0.46 +0.1 2,826,174 + Psest_2642 0.49 +0.2 2,826,224 + Psest_2642 0.55 -0.1 2,826,224 + Psest_2642 0.55 +0.2 2,826,224 + Psest_2642 0.55 -2.5 2,826,224 + Psest_2642 0.55 -2.1 2,826,224 + Psest_2642 0.55 +1.8 2,826,224 + Psest_2642 0.55 -0.6 2,826,224 + Psest_2642 0.55 -1.7 2,826,225 - Psest_2642 0.56 -1.0 2,826,225 - Psest_2642 0.56 -0.7 2,826,225 - Psest_2642 0.56 -0.5 2,826,225 - Psest_2642 0.56 -0.5 2,826,225 - Psest_2642 0.56 -0.3 2,826,225 - Psest_2642 0.56 +0.7 2,826,225 - Psest_2642 0.56 -2.0 2,826,225 - Psest_2642 0.56 -1.7 2,826,225 - Psest_2642 0.56 +2.0 2,826,225 - Psest_2642 0.56 -1.6 2,826,225 - Psest_2642 0.56 -1.3 2,826,543 - +0.2 2,826,543 - +1.4 2,826,556 + +0.6 2,826,642 - +1.3
Or see this region's nucleotide sequence