Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2537 and Psest_2538 are separated by 106 nucleotides Psest_2538 and Psest_2539 are separated by 24 nucleotides Psest_2539 and Psest_2540 are separated by 54 nucleotides Psest_2540 and Psest_2541 are separated by 27 nucleotides
Psest_2537: Psest_2537 - oxygen-independent coproporphyrinogen III oxidase, at 2,717,609 to 2,718,991
_2537
Psest_2538: Psest_2538 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 2,719,098 to 2,719,832
_2538
Psest_2539: Psest_2539 - adenine phosphoribosyltransferase, at 2,719,857 to 2,720,405
_2539
Psest_2540: Psest_2540 - Uncharacterized protein SCO1/SenC/PrrC, involved in biogenesis of respiratory and photosynthetic systems, at 2,720,460 to 2,721,068
_2540
Psest_2541: Psest_2541 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 2,721,096 to 2,721,797
_2541
Position (kb)
2719
2720
2721 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2718.947 kb on + strand at 2718.948 kb on - strand at 2718.962 kb on - strand at 2718.989 kb on + strand at 2719.028 kb on - strand at 2719.058 kb on + strand at 2719.072 kb on - strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.256 kb on - strand, within Psest_2538 at 2719.313 kb on + strand, within Psest_2538 at 2719.774 kb on + strand at 2719.775 kb on - strand at 2719.892 kb on - strand at 2719.940 kb on - strand, within Psest_2539 at 2720.145 kb on + strand, within Psest_2539 at 2720.145 kb on + strand, within Psest_2539 at 2720.293 kb on + strand, within Psest_2539 at 2720.294 kb on - strand, within Psest_2539 at 2720.432 kb on - strand at 2720.454 kb on + strand at 2720.455 kb on - strand at 2720.455 kb on - strand at 2720.567 kb on - strand, within Psest_2540 at 2720.567 kb on - strand, within Psest_2540 at 2720.663 kb on - strand, within Psest_2540 at 2720.702 kb on + strand, within Psest_2540 at 2720.728 kb on + strand, within Psest_2540 at 2720.770 kb on + strand, within Psest_2540 at 2720.820 kb on + strand, within Psest_2540 at 2720.820 kb on + strand, within Psest_2540 at 2720.820 kb on + strand, within Psest_2540 at 2720.821 kb on - strand, within Psest_2540 at 2720.821 kb on - strand, within Psest_2540 at 2720.821 kb on - strand, within Psest_2540 at 2720.821 kb on - strand, within Psest_2540 at 2720.821 kb on - strand, within Psest_2540 at 2720.821 kb on - strand, within Psest_2540 at 2720.829 kb on + strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.830 kb on - strand, within Psest_2540 at 2720.833 kb on - strand, within Psest_2540 at 2720.938 kb on - strand, within Psest_2540 at 2720.938 kb on - strand, within Psest_2540 at 2720.938 kb on - strand, within Psest_2540 at 2721.113 kb on + strand at 2721.123 kb on + strand at 2721.124 kb on - strand at 2721.124 kb on - strand at 2721.127 kb on + strand at 2721.127 kb on + strand at 2721.127 kb on + strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.128 kb on - strand at 2721.209 kb on + strand, within Psest_2541 at 2721.210 kb on - strand, within Psest_2541 at 2721.210 kb on - strand, within Psest_2541 at 2721.252 kb on + strand, within Psest_2541 at 2721.252 kb on + strand, within Psest_2541 at 2721.273 kb on - strand, within Psest_2541 at 2721.352 kb on - strand, within Psest_2541
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,718,947 + +1.0 2,718,948 - -1.3 2,718,962 - -0.1 2,718,989 + -0.7 2,719,028 - -0.0 2,719,058 + -0.2 2,719,072 - +0.1 2,719,097 + +1.8 2,719,097 + -0.6 2,719,097 + -0.1 2,719,097 + +0.5 2,719,097 + -1.1 2,719,097 + -1.6 2,719,097 + +0.3 2,719,098 - -0.9 2,719,098 - +2.2 2,719,098 - +1.4 2,719,098 - -0.4 2,719,098 - +1.9 2,719,098 - -1.1 2,719,098 - +0.5 2,719,256 - Psest_2538 0.21 -0.8 2,719,313 + Psest_2538 0.29 +1.1 2,719,774 + +0.6 2,719,775 - -3.1 2,719,892 - -0.2 2,719,940 - Psest_2539 0.15 +0.4 2,720,145 + Psest_2539 0.52 -0.4 2,720,145 + Psest_2539 0.52 +0.7 2,720,293 + Psest_2539 0.79 +3.1 2,720,294 - Psest_2539 0.80 -0.9 2,720,432 - -0.5 2,720,454 + +0.7 2,720,455 - -2.9 2,720,455 - -1.1 2,720,567 - Psest_2540 0.18 +1.8 2,720,567 - Psest_2540 0.18 +2.4 2,720,663 - Psest_2540 0.33 +1.5 2,720,702 + Psest_2540 0.40 +1.0 2,720,728 + Psest_2540 0.44 +0.5 2,720,770 + Psest_2540 0.51 +1.5 2,720,820 + Psest_2540 0.59 -1.1 2,720,820 + Psest_2540 0.59 +0.0 2,720,820 + Psest_2540 0.59 -1.1 2,720,821 - Psest_2540 0.59 -1.8 2,720,821 - Psest_2540 0.59 -0.9 2,720,821 - Psest_2540 0.59 +0.3 2,720,821 - Psest_2540 0.59 +1.9 2,720,821 - Psest_2540 0.59 -1.2 2,720,821 - Psest_2540 0.59 +1.1 2,720,829 + Psest_2540 0.61 +1.3 2,720,830 - Psest_2540 0.61 +2.8 2,720,830 - Psest_2540 0.61 -1.7 2,720,830 - Psest_2540 0.61 -2.3 2,720,830 - Psest_2540 0.61 +0.8 2,720,830 - Psest_2540 0.61 -2.2 2,720,830 - Psest_2540 0.61 -0.3 2,720,830 - Psest_2540 0.61 +1.3 2,720,833 - Psest_2540 0.61 -1.1 2,720,938 - Psest_2540 0.78 -0.3 2,720,938 - Psest_2540 0.78 -2.9 2,720,938 - Psest_2540 0.78 +1.8 2,721,113 + -0.5 2,721,123 + +1.1 2,721,124 - -0.4 2,721,124 - -1.3 2,721,127 + +2.3 2,721,127 + +0.1 2,721,127 + +1.3 2,721,128 - +0.9 2,721,128 - +0.1 2,721,128 - +0.2 2,721,128 - +0.3 2,721,128 - -0.8 2,721,128 - -0.2 2,721,128 - -0.2 2,721,128 - +1.3 2,721,128 - -0.1 2,721,209 + Psest_2541 0.16 -0.4 2,721,210 - Psest_2541 0.16 +1.2 2,721,210 - Psest_2541 0.16 -3.1 2,721,252 + Psest_2541 0.22 -0.0 2,721,252 + Psest_2541 0.22 -1.5 2,721,273 - Psest_2541 0.25 -0.7 2,721,352 - Psest_2541 0.36 -1.6
Or see this region's nucleotide sequence