Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2436

Experiment: Bacitracin 0.25 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2435 and Psest_2436 are separated by 156 nucleotidesPsest_2436 and Psest_2437 are separated by 47 nucleotidesPsest_2437 and Psest_2438 are separated by 36 nucleotides Psest_2435: Psest_2435 - Transcriptional regulator, at 2,602,886 to 2,603,776 _2435 Psest_2436: Psest_2436 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (from data), at 2,603,933 to 2,605,444 _2436 Psest_2437: Psest_2437 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (from data), at 2,605,492 to 2,606,265 _2437 Psest_2438: Psest_2438 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) (from data), at 2,606,302 to 2,607,192 _2438 Position (kb) 2603 2604 2605 2606Strain fitness (log2 ratio) -1 0 1 2 3at 2602.936 kb on - strandat 2603.205 kb on + strand, within Psest_2435at 2603.205 kb on + strand, within Psest_2435at 2603.206 kb on - strand, within Psest_2435at 2603.206 kb on - strand, within Psest_2435at 2603.206 kb on - strand, within Psest_2435at 2603.268 kb on + strand, within Psest_2435at 2603.268 kb on + strand, within Psest_2435at 2603.269 kb on - strand, within Psest_2435at 2603.283 kb on + strand, within Psest_2435at 2603.283 kb on + strand, within Psest_2435at 2603.284 kb on - strand, within Psest_2435at 2603.284 kb on - strand, within Psest_2435at 2603.284 kb on - strand, within Psest_2435at 2603.284 kb on - strand, within Psest_2435at 2603.614 kb on - strand, within Psest_2435at 2603.754 kb on + strandat 2603.754 kb on + strandat 2603.755 kb on - strandat 2603.839 kb on + strandat 2603.861 kb on - strandat 2603.933 kb on - strandat 2603.933 kb on - strandat 2603.933 kb on - strandat 2603.956 kb on + strandat 2604.164 kb on + strand, within Psest_2436at 2604.164 kb on + strand, within Psest_2436at 2604.165 kb on - strand, within Psest_2436at 2604.165 kb on - strand, within Psest_2436at 2604.165 kb on - strand, within Psest_2436at 2604.165 kb on - strand, within Psest_2436at 2604.350 kb on + strand, within Psest_2436at 2604.351 kb on - strand, within Psest_2436at 2604.351 kb on - strand, within Psest_2436at 2604.351 kb on - strand, within Psest_2436at 2604.351 kb on - strand, within Psest_2436at 2604.351 kb on - strand, within Psest_2436at 2604.351 kb on - strand, within Psest_2436at 2604.351 kb on - strand, within Psest_2436at 2604.651 kb on - strand, within Psest_2436at 2604.651 kb on - strand, within Psest_2436at 2605.083 kb on - strand, within Psest_2436at 2605.260 kb on + strand, within Psest_2436at 2605.358 kb on + strandat 2605.358 kb on + strandat 2605.359 kb on - strandat 2605.359 kb on - strandat 2605.359 kb on - strandat 2605.359 kb on - strandat 2605.542 kb on + strandat 2605.714 kb on + strand, within Psest_2437at 2605.726 kb on + strand, within Psest_2437at 2605.727 kb on - strand, within Psest_2437at 2605.727 kb on - strand, within Psest_2437at 2605.898 kb on + strand, within Psest_2437at 2606.280 kb on + strandat 2606.316 kb on + strandat 2606.317 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml
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2,602,936 - +0.2
2,603,205 + Psest_2435 0.36 +1.4
2,603,205 + Psest_2435 0.36 -1.5
2,603,206 - Psest_2435 0.36 -0.2
2,603,206 - Psest_2435 0.36 +2.9
2,603,206 - Psest_2435 0.36 -1.6
2,603,268 + Psest_2435 0.43 +1.8
2,603,268 + Psest_2435 0.43 -0.9
2,603,269 - Psest_2435 0.43 +1.8
2,603,283 + Psest_2435 0.45 -1.1
2,603,283 + Psest_2435 0.45 -0.2
2,603,284 - Psest_2435 0.45 -1.5
2,603,284 - Psest_2435 0.45 -0.9
2,603,284 - Psest_2435 0.45 +0.6
2,603,284 - Psest_2435 0.45 +1.4
2,603,614 - Psest_2435 0.82 +0.2
2,603,754 + -0.4
2,603,754 + -1.2
2,603,755 - -0.2
2,603,839 + +0.8
2,603,861 - +0.6
2,603,933 - +1.2
2,603,933 - +0.1
2,603,933 - +1.3
2,603,956 + -0.6
2,604,164 + Psest_2436 0.15 +0.5
2,604,164 + Psest_2436 0.15 +2.7
2,604,165 - Psest_2436 0.15 +1.1
2,604,165 - Psest_2436 0.15 +0.2
2,604,165 - Psest_2436 0.15 +2.1
2,604,165 - Psest_2436 0.15 +0.4
2,604,350 + Psest_2436 0.28 -0.4
2,604,351 - Psest_2436 0.28 +0.6
2,604,351 - Psest_2436 0.28 +1.2
2,604,351 - Psest_2436 0.28 -0.5
2,604,351 - Psest_2436 0.28 -0.3
2,604,351 - Psest_2436 0.28 +0.7
2,604,351 - Psest_2436 0.28 +1.0
2,604,351 - Psest_2436 0.28 +0.7
2,604,651 - Psest_2436 0.47 -0.5
2,604,651 - Psest_2436 0.47 +2.2
2,605,083 - Psest_2436 0.76 -1.3
2,605,260 + Psest_2436 0.88 +0.3
2,605,358 + +0.3
2,605,358 + -0.5
2,605,359 - +1.5
2,605,359 - -0.2
2,605,359 - +0.6
2,605,359 - +0.7
2,605,542 + +1.9
2,605,714 + Psest_2437 0.29 +0.6
2,605,726 + Psest_2437 0.30 +0.8
2,605,727 - Psest_2437 0.30 +0.6
2,605,727 - Psest_2437 0.30 +0.8
2,605,898 + Psest_2437 0.52 -0.6
2,606,280 + +0.5
2,606,316 + -1.5
2,606,317 - -0.4

Or see this region's nucleotide sequence