Experiment: Bacitracin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2435 and Psest_2436 are separated by 156 nucleotides Psest_2436 and Psest_2437 are separated by 47 nucleotides Psest_2437 and Psest_2438 are separated by 36 nucleotides
Psest_2435: Psest_2435 - Transcriptional regulator, at 2,602,886 to 2,603,776
_2435
Psest_2436: Psest_2436 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (from data) , at 2,603,933 to 2,605,444
_2436
Psest_2437: Psest_2437 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (from data) , at 2,605,492 to 2,606,265
_2437
Psest_2438: Psest_2438 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) (from data) , at 2,606,302 to 2,607,192
_2438
Position (kb)
2603
2604
2605
2606 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2602.936 kb on - strand at 2603.205 kb on + strand, within Psest_2435 at 2603.205 kb on + strand, within Psest_2435 at 2603.206 kb on - strand, within Psest_2435 at 2603.206 kb on - strand, within Psest_2435 at 2603.206 kb on - strand, within Psest_2435 at 2603.268 kb on + strand, within Psest_2435 at 2603.268 kb on + strand, within Psest_2435 at 2603.269 kb on - strand, within Psest_2435 at 2603.283 kb on + strand, within Psest_2435 at 2603.283 kb on + strand, within Psest_2435 at 2603.284 kb on - strand, within Psest_2435 at 2603.284 kb on - strand, within Psest_2435 at 2603.284 kb on - strand, within Psest_2435 at 2603.284 kb on - strand, within Psest_2435 at 2603.614 kb on - strand, within Psest_2435 at 2603.754 kb on + strand at 2603.754 kb on + strand at 2603.755 kb on - strand at 2603.839 kb on + strand at 2603.861 kb on - strand at 2603.933 kb on - strand at 2603.933 kb on - strand at 2603.933 kb on - strand at 2603.956 kb on + strand at 2604.164 kb on + strand, within Psest_2436 at 2604.164 kb on + strand, within Psest_2436 at 2604.165 kb on - strand, within Psest_2436 at 2604.165 kb on - strand, within Psest_2436 at 2604.165 kb on - strand, within Psest_2436 at 2604.165 kb on - strand, within Psest_2436 at 2604.350 kb on + strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.651 kb on - strand, within Psest_2436 at 2604.651 kb on - strand, within Psest_2436 at 2605.083 kb on - strand, within Psest_2436 at 2605.260 kb on + strand, within Psest_2436 at 2605.358 kb on + strand at 2605.358 kb on + strand at 2605.359 kb on - strand at 2605.359 kb on - strand at 2605.359 kb on - strand at 2605.359 kb on - strand at 2605.542 kb on + strand at 2605.714 kb on + strand, within Psest_2437 at 2605.726 kb on + strand, within Psest_2437 at 2605.727 kb on - strand, within Psest_2437 at 2605.727 kb on - strand, within Psest_2437 at 2605.898 kb on + strand, within Psest_2437 at 2606.280 kb on + strand at 2606.316 kb on + strand at 2606.317 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.25 mg/ml remove 2,602,936 - +0.2 2,603,205 + Psest_2435 0.36 +1.4 2,603,205 + Psest_2435 0.36 -1.5 2,603,206 - Psest_2435 0.36 -0.2 2,603,206 - Psest_2435 0.36 +2.9 2,603,206 - Psest_2435 0.36 -1.6 2,603,268 + Psest_2435 0.43 +1.8 2,603,268 + Psest_2435 0.43 -0.9 2,603,269 - Psest_2435 0.43 +1.8 2,603,283 + Psest_2435 0.45 -1.1 2,603,283 + Psest_2435 0.45 -0.2 2,603,284 - Psest_2435 0.45 -1.5 2,603,284 - Psest_2435 0.45 -0.9 2,603,284 - Psest_2435 0.45 +0.6 2,603,284 - Psest_2435 0.45 +1.4 2,603,614 - Psest_2435 0.82 +0.2 2,603,754 + -0.4 2,603,754 + -1.2 2,603,755 - -0.2 2,603,839 + +0.8 2,603,861 - +0.6 2,603,933 - +1.2 2,603,933 - +0.1 2,603,933 - +1.3 2,603,956 + -0.6 2,604,164 + Psest_2436 0.15 +0.5 2,604,164 + Psest_2436 0.15 +2.7 2,604,165 - Psest_2436 0.15 +1.1 2,604,165 - Psest_2436 0.15 +0.2 2,604,165 - Psest_2436 0.15 +2.1 2,604,165 - Psest_2436 0.15 +0.4 2,604,350 + Psest_2436 0.28 -0.4 2,604,351 - Psest_2436 0.28 +0.6 2,604,351 - Psest_2436 0.28 +1.2 2,604,351 - Psest_2436 0.28 -0.5 2,604,351 - Psest_2436 0.28 -0.3 2,604,351 - Psest_2436 0.28 +0.7 2,604,351 - Psest_2436 0.28 +1.0 2,604,351 - Psest_2436 0.28 +0.7 2,604,651 - Psest_2436 0.47 -0.5 2,604,651 - Psest_2436 0.47 +2.2 2,605,083 - Psest_2436 0.76 -1.3 2,605,260 + Psest_2436 0.88 +0.3 2,605,358 + +0.3 2,605,358 + -0.5 2,605,359 - +1.5 2,605,359 - -0.2 2,605,359 - +0.6 2,605,359 - +0.7 2,605,542 + +1.9 2,605,714 + Psest_2437 0.29 +0.6 2,605,726 + Psest_2437 0.30 +0.8 2,605,727 - Psest_2437 0.30 +0.6 2,605,727 - Psest_2437 0.30 +0.8 2,605,898 + Psest_2437 0.52 -0.6 2,606,280 + +0.5 2,606,316 + -1.5 2,606,317 - -0.4
Or see this region's nucleotide sequence